CBF冷应答途径基因在热处理诱导的香蕉果实抗冷性中的作用

doi:10.3864/j.issn.0578-1752.2016.14.010

摘要/Abstract

摘要： 【目的】探讨CBF/DREB（C-repeat binding transcription factor/dehydrate responsive element binding factor）冷应答途径在热处理诱导香蕉抗冷性中的作用，为研究香蕉果实中CBF冷应答途径的信号转导过程提供参考依据。【方法】从香蕉基因组数据库（http://banana-hub.southgreen.fr/）中挑选CBF冷应答途径的7个基因序列，分别设计特异引物，利用实时荧光定量 PCR分析这7个基因在热处理诱导的香蕉抗冷性中的表达模式。【结果】香蕉果实MaICE在7℃处理1 h时，表达迅速升高并达到最大值，DREB类基因（MaDREB1D、MaDREB1E、MaDREB1G和MaDREB3）在7℃处理1 h时，均存在一个表达高峰，MaCOR413在7℃下4 h时有一个表达高峰。这些结果表明，香蕉果实中在7℃低温胁迫下中存在CBF冷应答途径，即ICE(inducer of CBF expression）-CBF-COR(Cold-regulated genes)通路。香蕉果实CBF冷应答途径的7个基因表达均在热处理（52℃，3 min）后0.5 h迅速达到最大值，进一步研究发现，香蕉果实经热处理后于7℃低温贮藏5 d时，MaDREB1D、MaDREB1E、MaDREB2C、MaDREB3和MaCOR413的表达均强于对照。【结论】7℃低温胁迫下MaICE、DREB类基因、MaCOR413表达依次增强，说明低温下香蕉果实中存在CBF冷应答途径。CBF冷应答途径相关基因表达增强可能参与了热处理诱导采后香蕉果实的抗冷性。

关键词: CBF/DREB, 香蕉果实, 热处理, 抗冷性

Abstract: 【Objective】The objective of this study is to investigate the role of C-repeat binding transcription factor (CBF) cold-resistance pathway in heat treatment -induced chilling tolerance in banana fruits and to provide reference for the study of the signal transduction of CBF cold response pathway in banana fruits.【Method】The sequences of 7 genes related to CBF cold response pathway were selected from the banana genome database (http://banana-genome-hub.southgreen.fr/), and then specific primers were designed respectively. Analysis of these 7 genes’ expression patterns of the heat-induced chilling tolerance in banana fruits was conducted using quantitative real-time PCR.【Result】The expression of MaICE gene of banana fruit increased rapidly and reached the maximum level at 7℃ for 1 hour. The expression of DREB (MaDREB1D, MaDREB1E, MaDREB1G and MaDREB3) genes displayed a peak at 7℃ for 1 hour. The expression of MaCOR413 gene had a peak at 7℃ for 4 hours. These results indicated that the CBF cold response pathway (ICE-CBF-COR pathway) exist in banana fruit during cold storage at 7℃. The expression of 7 gene related to CBF cold response pathway reached the maximum levels at 0.5 hour after heat treatment (52℃ 3 min). Furthermore, when the heat treated banana fruits storage at 7℃ for 5 days, the expressions of MaDREB1D, MaDREB1E, MaDREB2C, MaDREB3 and MaCOR413 genes were higher than the non-heated control.【Conclusion】 MaICE, DREB, and MaCOR413 increased in turn when banana fruits stored at 7℃. The CBF cold response pathway exist in banana fruits during cold storage at 7℃. Enhancement of gene expression related to CBF cold response pathway may be involved in chilling tolerance induced by heat treatment in banana fruits.

Key words: CBF/DREB, banana fruit, heat treatment, chilling tolerance

王海波，李璐，苏新国，张昭其，庞学群. CBF冷应答途径基因在热处理诱导的香蕉果实抗冷性中的作用[J]. 中国农业科学, 2016, 49(14): 2763-2771.

WANG Hai-bo, LI Lu, SU Xin-guo, ZHANG Zhao-qi, PANG Xue-qun. The Role of CBF Cold Response Pathway Gene in Heat Treatment-Induced Chilling Tolerance in Banana Fruits[J]. Scientia Agricultura Sinica, 2016, 49(14): 2763-2771.

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