玉米大斑病菌MAPK超家族的全基因组鉴定及途径模型建立

doi:10.3864/j.issn.0578-1752.2014.09.006

中国农业科学 ›› 2014, Vol. 47 ›› Issue (9): 1715-1724.doi: 10.3864/j.issn.0578-1752.2014.09.006

玉米大斑病菌MAPK超家族的全基因组鉴定及途径模型建立

巩校东1, 张晓玉1, 田兰1, 王星懿1, 李坡2, 张盼1, 王玥1, 范永山3, 韩建民1, 谷守芹1, 董金皋1

1、河北农业大学真菌毒素与植物分子病理学实验室，河北保定 071001；
2、河北省农林科学院植物保护研究所，河北保定 071000；
3、唐山师范学院生命科学系，河北唐山 063000

收稿日期:2013-09-29 出版日期:2014-05-01 发布日期:2014-01-03
通讯作者: 谷守芹，E-mail：gushouqin@126.com；通信作者韩建民，E-mail：hanjmnd@163.com；通信作者董金皋，E-mail：dongjingao@126.com
作者简介:巩校东，E-mail：gxdjy123@gmail.com；张晓玉，E-mail：aishu123@126.com。巩校东与张晓玉为同等贡献作者
基金资助:
国家自然科学基金项目（31171805，31271997，31371897）、河北省自然科学基金项目（C2012204033，C2013204061）

Genome-Wide Identification MAPK Superfamily and Establishment of the Model of MAPK Cascade Pathway in Setosphaeria turcica

GONG Xiao-Dong-1, ZHANG Xiao-Yu-1, TIAN Lan-1, WANG Xing-Yi-1, LI Po-2, ZHANG Pan-1, WANG Yue-1, FAN Yong-Shan-3, HAN Jian-Min-1, GU Shou-Qin-1, DONG Jin-Gao-1

1、Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071001, Hebei;
2、Plant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences, Baoding 071000, Hebei;
3、Faculty of Life Science, Tangshan Normal University, Tangshan 063000, Hebei

Received:2013-09-29 Online:2014-05-01 Published:2014-01-03

摘要/Abstract

摘要： 【目的】从全基因组水平鉴定玉米大斑病菌（Setosphaeria turcica）的MAPK超家族基因，并对其进行系统进化、基因结构、多重序列比对及保守位点分析，构建玉米大斑病菌中的MAPK级联途径模型，为深入研究该植物病原菌中MAPK级联途径的功能奠定基础。【方法】利用玉米大斑病菌基因组数据库，通过基于隐马尔科夫模型的HMMER 3.0软件搜索基因组，鉴定并获得MAPK超家族成员序列、基因组定位信息；采用MEGA 5.0软件进行系统进化分析；通过GSDS工具进行基因结构分析；利用ClustalX、MEME工具分析MAPK蛋白激酶区的保守性及保守位点。【结果】在玉米大斑病菌基因组中发现了4个MAPK基因、3个MAPKK基因和3个MAPKKK基因。系统进化分析将MAPK分为Kss1/Fus3、Slt2、Hog1及Ime2 4类；MAPKK分为Pbs2、Ste7及Mkk1 3类；MAPKKK分为Ste11、Bck1及Ssk2 3类。基因组定位及基因结构分析表明，MAPK超家族散布在基因组中，且MAPK基因的结构最为复杂多样，MAPKK次之，MAPKKK结构最简单。多重序列比对与保守位点分析表明，玉米大斑病菌MAPK超家族的激酶结构域均高度保守，其中MAPK具有保守的“-TxY-”磷酸化位点，MAPKK含有保守的“-SD[V/I]WS-”磷酸化位点，MAPKKK含有“-G[S/T][V/P][F/M][W/Y]M[A/S]PEV-”特异性保守位点。【结论】全基因组分析表明，玉米大斑病菌中包括4个MAPK基因、3个MAPKK基因和3个MAPKKK基因。通过系统进化、基因结构及多重序列比对和保守位点分析，在玉米大斑病菌中构建了Fus3/Kss1-homolog、Slt2-homolog、Hog1-homolog和Ime2-homolog 4条MAPK级联途径，其中Ime2 homolog为一条新发现的MAPK级联途径。该信息为深入解析植物病原真菌MAPK超家族的功能奠定了基础。

关键词: 玉米大斑病菌 , MAPK基因超家族 , 系统进化 , 保守基序

Abstract: 【Objective】 Identification of MAPK superfamily genes from wide genome, and analysis of phylogeny, gene structure, multiple sequence alignment and conservative sites in Setosphaeria turcica will be useful for further research on the functions of MAPK cascade pathway in plant pathogenic fungus.【Method】Based on S. turcica genome database and genome searching with HMMER 3.0, MAPK superfamily genes were identified and their gene sequences, and genome location were obtained. The conservative protein kinase domains and sites were analyzed with ClustalX, MEME tools.【Result】Four MAPK genes, three MAPKK genes and three MAPKKK genes in S. turcica genome were found, and the MAPK was classified into Kss1/Fus3, Slt2, Hog1 and Ime2 four classes, MAPKK was classified into Pbs2, Ste7 and Mkk1 three classes, MAPKKK was classified into Ste11, Bck1 and Ssk2 three classes. Genome location and gene structure analysis showed that MAPK superfamily genes extensively distributed in the genome, and the structure of MAPK genes was the most complicated, MAPKK taken the second place, and MAPKKK structure was the simplest. Multiple sequence alignment and conservative sites analysis showed that the MAPK superfamily of kinase domain structure was highly conserved in S. turcica, and MAPK contained phosphorylation sites (-TxY-), MAPKK contained conservative phosphorylation sites (-SD[V/I]WS-), MAPKKK contained specificity conservative sites (-G[S/T] [V/P][F/M][W/Y]M[A/S]PEV-).【Conclusion】Genome-wide analysis showed that there were four MAPK genes, three MAPKK genes and three MAPKKK genes in S. turcica. Phylogenetic, gene structure, multiple sequence alignment and conservative site analysis showed that there were Fus3/Kss1-homolog, Slt2-homolog, Hog1-homolog and Ime2-homolog four MAPK cascade pathways, and Ime2 homolog may be a newly discovered MAPK cascade pathway in S. turcica. These results are helpful for the further functional research of MAPK superfamily in plant pathogenic fungus.

Key words: Setosphaeria turcica , MAPK superfamily , phylogenetic analysis , conservative motif

巩校东1, 张晓玉1, 田兰1, 王星懿1, 李坡2, 张盼1, 王玥1, 范永山3, 韩建民1, 谷守芹1, 董金皋1. 玉米大斑病菌MAPK超家族的全基因组鉴定及途径模型建立[J]. 中国农业科学, 2014, 47(9): 1715-1724.

GONG Xiao-Dong-1, ZHANG Xiao-Yu-1, TIAN Lan-1, WANG Xing-Yi-1, LI Po-2, ZHANG Pan-1, WANG Yue-1, FAN Yong-Shan-3, HAN Jian-Min-1, GU Shou-Qin-1, DONG Jin-Gao-1. Genome-Wide Identification MAPK Superfamily and Establishment of the Model of MAPK Cascade Pathway in Setosphaeria turcica[J]. Scientia Agricultura Sinica, 2014, 47(9): 1715-1724.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2014.09.006

https://www.chinaagrisci.com/CN/Y2014/V47/I9/1715

参考文献

[1]Perkins J M, Pedersen W L. Disease development and yield losses associated with leaf northern blight on corn. Plant Disease, 1987, 71(10): 940-943.

[2]刘国胜, 董金皋, 邓福友, 郭爱国, 张凤国, 臧漫辉. 中国玉米大斑病菌生理分化及新命名法的初步研究. 植物病理学报, 1996, 26(4): 305-310.

Liu G S, Dong J G, Deng F Y, Guo A G, Zhang F G, Zang M H. Preliminary study on physiology specialization and new nomenclature for Exserohilum turcicum of corn in China. Acta Phytopathologica Sinica, 1996, 26(4): 305-310. (in Chinese)

[3]Romeis T. Protein kinases in the plant defence response. Current Opinion in Plant Biology, 2001, 4: 407-414.

[4]Yang S H, Sharrocks A D, Whitmarsh A J. Transcriptional regulation by the MAP kinase signaling cascades. Gene, 2003, 320: 3-21.

[5]Chen R E, Thorner J. Function and regulation in MAPK signaling pathways: Lessons learned from the yeast Saccharomyces cerevisiae. Biochimica et Biophysica Acta, 2007, 1773(8): 1311-1340.

[6]范永山, 刘颖超, 谷守芹, 桂秀梅, 董金皋. 植物病原真菌的MAPK基因及其功能. 微生物学报, 2004, 44(4): 547-551.

Fan Y S, Liu Y C, Gu S Q, Gui X M, Dong J G. Mitogen activated protein kinase genes and its functions in phytopathogenic fungus. Acta Microbiologica Sinica, 2004, 44(4): 547-551. (in Chinese)

[7]Herskowitz I. MAP kinase pathways in yeast: For mating and more. Cell, 1995, 80(2): 187-197.

[8]Hamel L P, Nicole M C, Duplessis S, Ellis B E. Mitogen-activated protein kinase signaling in plant-interacting fungi: distinct messages from conserved messengers. The Plant Cell, 2012, 24: 1327-1351.

[9]Xu J R. MAP kinases in fungal pathogens. Fungal Genetics and Biology, 2000, 31: 137-152.

[10]Banuett F, Herskowitz I. Identification of fuz7, a Ustilago maydis MEK/MAPKK homolog required for a-locus-dependent and -independent steps in the fungal life cycle. Genes & Development, 1994, 8: 1367-1378.

[11]Carbó N, Pérez-Martín J. Activation of the cell wall integrity pathway promotes escape from G2 in the fungus Ustilago maydis. PLoS Genetics, 2010, 6(7): e1001009.

[12]Belén Sanz A, García R, Rodríguez-Peña J M, Díez-Muñiz S, Nombela C, Peterson C L, Arroyo J. Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway. Molecular Biology of the Cell, 2012, 23: 2805-2817.

[13]Segmüller N, Ellendorf U, Tudzynski B, Tudzynski P. BcSAK1, a stress-activated mitogen-activated protein kinase, is involved in vegetative differentiation and pathogenicity in Botrytis cinerea. Eukaryotic Cell, 2007, 6(2): 211-221.

[14]Park S M, Choi E S, Kim M J, Cha B J, Yang M S, Kim D H. Characterization of HOG1 homologue, CpMK1, from Cryphonectria parasitica and evidence for hypovirus-mediated perturbation of its phosphorylation in response to hypertonic stress. Molecular Microbiology, 2004, 51(5): 1267-1277.

[15]王宁, 谷守芹, 范永山, 李坡, 王文秀, 董金皋. 玉米大斑病菌STK1原核表达载体的构建及其表达. 中国农业科学, 2010, 43(18): 3876-3881.

Wang N, Gu S Q, Fan Y S, Li P, Wang W X, Dong J G. Construction and expression of prokaryotic expression vector of STK1 from Setosphaeria turcica. Scientia Agricultura Sinica, 2010, 43(18): 3876-3881. (in Chinese)

[16]巩校东, 范钰, 李坡, 杨阳, 张长志, 田兰, 张晓玉, 范永山, 韩建民, 谷守芹, 董金皋. 玉米大斑病菌STK2的基因组定位、蛋白质结构预测及其表达. 中国农业科学, 2013, 46(12): 2599-2606.

Gong X D, Fan Y, Li P, Yang Y, Zhang C Z, Tian L, Zhang X Y, Fan Y S, Han J M, Gu S Q, Dong J G. Localization of STK2 of Setosphaeria turcica in the genome, characterization of its protein structure and expression in eukaryotic cells. Scientia Agricultura Sinica, 2013, 46(12): 2599-2606. (in Chinese)

[17]Ohm R A, Feau N, Henrissat B, Schoch C L, Horwitz B A, Barry K W, Condon B J, Copeland A C, Dhillon B, Glaser F, Hesse C N, Kosti I, LaButti K, Lindquist E A, Lucas S, Salamov A A, Bradshaw R E, Ciuffetti L, Hamelin R C, Kema G H, Lawrence C, Scott J A, Spatafora J W, Turgeon B G, de Wit P J, Zhong S, Goodwin S B, Grigoriev I V. Diverse lifestyles and strategies of plant pathogenesis encoded in the genomes of eighteen Dothideomycetes fungi. PLoS Pathogens, 2012, 8(12): e1003037.

[18]Hamel L P, Nicole M C, Sritubtim S, Morency M J, Ellis M, Ehlting J, Beaudoin N, Barbazuk B, Klessig D, Lee J, Martin G, Mundy J, Ohashi Y, Scheel D, Sheen J, Xing T, Zhang S, Seguin A, Ellis B E. Ancient signals: comparative genomics of plant MAPK and MAPKK gene families. Trends in Plant Science, 2006, 11(4): 192-198.

[19]郭安源, 朱其慧, 陈新, 罗静初. GSDS: 基因结构显示系统. 遗传, 2007, 29(8): 1023-1026.

Guo A Y, Zhu Q H, Chen X, Luo J C. GSDS: a gene structure display server. Hereditas, 2007, 29(8): 1023-1026. (in Chinese)

[20]Liang W, Yang B, Yu B J, Zhou Z, Li C, Jia M, Sun Y, Zhang Y, Wu F, Zhang H, Wang B, Deyholos M K, Jiang Y Q. Identification and analysis of MKK and MPK gene families in canola (Brassica napus L.). BMC Genomics, 2013, 14: 392.

[21]Chen L, Hu W, Tan S, Wang M, Ma Z, Zhou S, Deng X, Zhang Y, Huang C, Yang G, He G. Genome-wide identification and analysis of MAPK and MAPKK gene families in Brachypodium distachyon. PLoS One, 2012, 7(10): e46744.

[22]Rao K P, Richa T, Kumar K, Raghuram B, Sinha A K. In silico analysis reveals 75 members of mitogen-activated protein kinase kinase kinase genefamily in rice. DNA Research, 2010, 17(3): 139-153.

[23]Kong X, Lv W, Zhang D, Jiang S, Zhang S, Li D. Genome-wide identification and analysis of expression profiles of maize mitogen- activated protein kinase kinase kinase. PLoS One, 2013, 8(2): e57714.

[24]Zhao X H, Kim Y, Park G, Xu J R. A Mitogen-activated protein kinase cascade regulating infection-related morphogenesis in Magnaporthe grisea. The Plant Cell, 2005, 17: 1317-1329.

[25]Hou Z, Xue C, Peng Y, Katan T, Kistler H C, Xu J R. A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection. Molecular Plant-Microbe Interactions, 2002, 15(11): 1119-1127.

[26]Jeon J, Goh J, Yoo S, Chi M H, Choi J, Rho H S, Park J, Han S S, Kim B R, Park S Y, Kim S, Lee Y H. A putative MAP kinase kinase kinase, MCK1, is required for cell wall integrity and pathogenicity of the rice blast fungus, Magnaporthe oryza. Molecular Plant-Microbe Interactions, 2008, 21(5): 525-534.

[27]Guttmann-Raviv N, Martin S, Kassir Y. Ime2, a meiosis-specific kinase in yeast, is required for destabilization of its transcriptional activator, Ime1. Molecular and Cellular Biology, 2002, 22(7): 2047-2056.

[28]Irniger S. The Ime2 protein kinase family in fungi: more duties than just meiosis. Molecular Microbiology, 2011, 80(1): 1-13.

[29]Schindler K, Benjamin K R, Martin A, Boglioli A, Herskowitz I, Winter E. The Cdk-activating kinase Cak1p promotes meiotic S phase through Ime2p. Molecular and Cellular Biology, 2003, 23(23): 8718-8728.

[30]Garrido E, Voss U, Müller P, Castillo-Lluva S, Kahmann R, Pérez-Martín J. The induction of sexual development and virulence in the smut fungus Ustilago maydis depends on Crk1, a novel MAPK protein. Genes & Development, 2004, 18: 3117-3130.

玉米大斑病菌MAPK超家族的全基因组鉴定及途径模型建立

Genome-Wide Identification MAPK Superfamily and Establishment of the Model of MAPK Cascade Pathway in Setosphaeria turcica

RichHTML

PDF

赞

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	郝玉彬,李海笑,张赛,刘宁,刘英姿,曹志艳,董金皋. 玉米大斑病菌小柱孢酮脱水酶StSCD家族鉴定及其功能分析[J]. 中国农业科学, 2022, 55(16): 3134-3143.
[2]	李天聪,朱行,魏宁,龙凤,武建颖,张燕,董金皋,申珅,郝志敏. 玉米大斑病菌SC35同源基因表达规律与互作分析[J]. 中国农业科学, 2021, 54(4): 733-743.
[3]	邵晨冰,黄志楠,白雪滢,王云鹏,段伟科. 辣椒HD-Zip基因家族鉴定、系统进化及表达分析[J]. 中国农业科学, 2020, 53(5): 1004-1017.
[4]	代玉立,甘林,滕振勇,杨静民,祁月月,石妞妞,陈福如,杨秀娟. 玉米大斑病菌和小斑病菌交配型多重PCR 检测方法的建立与应用[J]. 中国农业科学, 2020, 53(3): 527-538.
[5]	龙凤,王擎,朱行,王建霞,申珅,刘宁,郝志敏,董金皋. 玉米大斑病菌Septin基因家族的鉴定与表达模式分析[J]. 中国农业科学, 2020, 53(24): 5017-5026.
[6]	刘海璐，王暄，李红梅，李艳霞，薛博文，马居奎. 我国黄淮麦区10个短体线虫样品种类的分子鉴定[J]. 中国农业科学, 2018, 51(15): 2898-2912.
[7]	许瑞瑞, 李睿, 王小非, 郝玉金. 苹果OFP基因家族的全基因组鉴定与非生物逆境表达分析[J]. 中国农业科学, 2018, 51(10): 1948-1959.
[8]	冯胜泽，刘星晨，王海祥，赵洁，赵立卿，郑亚男，巩校东，韩建民，谷守芹，董金皋. 玉米大斑病菌分生孢子形成的影响因素及GATA 转录因子家族的表达分析[J]. 中国农业科学, 2017, 50(7): 1234-1241.
[9]	赵洁，赵立卿，巩校东，冯胜泽，刘星晨，郑亚男，李志勇，孙海月，王冬雪，韩建民，谷守芹，董金皋. 玉米大斑病菌Homeobox转录因子家族全基因组鉴定及其表达规律分析[J]. 中国农业科学, 2017, 50(4): 669-678.
[10]	申珅, 李贞杨, 赵玉兰, 李盼, 韩建民, 郝志敏, 董金皋. 玉米大斑病菌环腺苷酸磷酸二酯酶基因克隆及表达分析[J]. 中国农业科学, 2017, 50(16): 3135-3144.
[11]	张运峰，张淑红，武秋颖，范永山. STK1对玉米大斑病菌附着胞发育过程中糖原和脂肪积累的影响[J]. 中国农业科学, 2017, 50(15): 2928-2935.
[12]	王烨，韩蕾，董捷，黄家兴，吴杰. 兰州熊蜂气味受体家族鉴定及分析[J]. 中国农业科学, 2017, 50(10): 1904-1913.
[13]	巩校东，刘星晨，赵立卿，郑亚男，范永山，韩建民，谷守芹，董金皋. 高渗胁迫对玉米大斑病菌生长发育的影响及菌丝细胞中渗透调节物质的分析[J]. 中国农业科学, 2017, 50(10): 1922-1929.
[14]	马双新，刘宁，贾慧，戴冬青，许苗苗，曹志艳，董金皋. 玉米大斑病菌漆酶基因Stlac2结构分析及原核表达[J]. 中国农业科学, 2016, 49(21): 4130-4139.
[15]	吴向阳，程朝泽，吕高强，王心宇. 芝麻AQP家族的全基因组序列鉴定及其特征分析[J]. 中国农业科学, 2016, 49(10): 1844-1858.