褪黑激素对PDGFA基因在绒山羊皮肤毛囊中表达模式的影响

doi:10.3864/j.issn.0578-1752.2012.10.016

摘要/Abstract

摘要： 【目的】探讨血小板衍生生长因子A（platelet-derived growth factor A，PDGFA）在内蒙古阿尔巴斯绒山羊皮肤毛囊上的表达规律和褪黑激素对其的影响。【方法】选择体重和产绒量相当的阿尔巴斯绒山羊成年母羊，分为4组，即从不埋植的对照组和3组不同时间埋植褪黑激素的试验组。试验组在埋植期间每隔一个月按照2 mg?kg-1 BW的剂量在绒山羊耳后皮下埋植褪黑激素。采集12个月（从2009年8月至2010年7月）的受试羊体侧部皮肤样品，用定量逆转录PCR （qRT-PCR）检测皮肤中PDGFA基因的表达量。【结果】各组间PDGFA基因的表达模式不同。对照组在11月时其表达量为全年最低，在12月，随着毛囊周期性生长缓慢进入退行期，其PDGFA基因的表达量也缓慢上升，并在休止期（1—4月）高度表达。而开始埋植褪黑激素后，仅第二年埋植组在翌年1月时表达量显著提高（P<0.05）；在2月与连续两年埋植组一样，随着绒毛开始脱落，PDGFA基因的表达量也有下降趋势；到5月，其表达量显著低于1月（P<0.05），此时新一轮的绒毛已长出体表。停止埋植褪黑激素后，仅第一年埋植组翌年PDGFA基因的表达模式和毛囊生长周期与对照组基本一致。【结论】绒山羊皮肤组织当中PDGFA基因在毛囊生长期低表达，退行期和休止期高表达。埋植褪黑激素可缩短毛囊生长周期。PDGFA基因是一个控制毛囊从兴盛期向退行期和休止期转化的因子。

关键词: 褪黑激素, 血小板生长因子A基因, 皮肤毛囊, 绒毛生长周期

Abstract: 【Objective】The expression regulation of PDGFA gene in skin follicles of Inner Mongolia Cashmere goat and the influence of melatonin on it were studied. 【Method】 Eight Cashmere mature femal goats with similar live weight were selected, and devided into four groups: never implanted as the control group and three groups of implanted melatonin in different times. The melatonin at a dose of 2 mg?kg-1 was implanted behind ears per 2 months during the implanting period. The body skin was collected in 12 months (from August 2009 to July 2010), and PDGFA gene expression was detected by using qRT-PCR. 【Result】The implanting MT into Cashmere goat influenced its hair growth cycle. The expression model of PDGFA gene in the skin and the hair growth cycle was different among groups. The lowest expression of PDGFA gene was noticed in November in control group. However, in December, the expression of PDGFA gene improved slowly with the hair growth went into catagen, and during telogen (from January to April), it kept higher level expression. After starting implanting MT into Cashmere goat, for the group just implanted melatonin in the second year, the expression of PDGFA gene was significantly improved (P<0.05) in next January. Similar results were found in implanted group for two years, it was lower with wool shedding in February, it was significantly lower in May than in January (P<0.05), and some new wool appeared on the skin at the same time. After stopping implanting MT for Cashmere goat, the PDGFA gene expression model of the group just implanted melatonin in the first year was similar with the control group.【Conclusion】 PDGFA gene had low expression in the Cashmere goat hair growth phase, and high expression in catagen and telogen. Melatonin implanted into Cashmere goat shortened hair growth cycle. PDGFA gene is a factor controlling the trarsformation of Cashmere goat hair from growth phase to catagen and telogen.

Key words: melatonin, platelet-derived growth factor A gene, skin hair follicle, hair growth circle

丽春, 张文广, 杨文柱, 王志新, 张永斌, 高爱琴, 张燕军, 赵艳红, 苏蕊, 常子丽, 金鑫, 王鹏宇, 冯林, 刘慧, 武瑞兵, 王瑞军, 李金泉. 褪黑激素对PDGFA基因在绒山羊皮肤毛囊中表达模式的影响[J]. 中国农业科学, 2012, 45(10): 2031-2039.

LI Chun, ZHANG Wen-Guang, YANG Wen-Zhu, WANG Zhi-Xin, ZHANG Yong-Bin, GAO Ai-Qin, ZHANG Yan-Jun, ZHAO Yan-Hong, SU Rui, CHANG Zi-Li, JIN Xin, WANG Peng-Yu, FENG Lin, LIU Hui, WU Rui-Bing, WANG Rui-Jun, LI Jin-Quan. Influence of Melatonin Implanting on PDGFA Gene Expression in Skin Hair Follicle[J]. Scientia Agricultura Sinica, 2012, 45(10): 2031-2039.

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参考文献

[1]Karlsson L, Bondjers C, Betsholtz C. Roles for PDGF-A and sonic hedgehog in development of mesenchymal components of the hair follicle. Development, 1999, 126(12): 2611-2621.

[2]Rust C C, MeyerR K. Hair color, molf and testis size in male, sherttailed weasels treated with melatonin. Science, 1969, 165(3896): 921-922.

[3]Allain D, Rougeot J. Induction of autumn moult in mink (Mustela vison Peale and Beauvois) with melatonin. Reproductive Nutrition Developement, 1980, 20(1): 197-201.

[4]Martinet L, Allain D, Weiner C. Role of prolactin in the photoperiodic control of moulting in the mink (Mustela visori). Journal of Endocrinology, 1984, 103: 9-15.

[5]孔庆松, 刘志平, 景松岩, 张伟, 叶春艳. 褪黑素促进水貂冬皮早熟的研究. 野生动物, 1995(4): 22-26.

Kong Q S, Liu Z P, Jing S Y, Zhang W, Ye C X. The study for melatonin promoting the mink winter skin matured early. Wildlife Animal, 1995(4): 22-26. (in Chinese)

[6]柳建昌. 含褪黑素微型胶囊促使银狐毛皮提早成熟的效应. 动物学杂志, 1995, 30(4): 48-51

Liu J C. The effect of melatonin in the rubber capsuliform implant with special instrument on the moulting cycle of fox (Mulpes fulva). Journal of Zoology, 1995, 30(4): 48-51. (in Chinese)

[7]谢保胜, 杨占兴, 马武龙, 索南元旦. 褪黑素对门源牦牛春季促绒生长及绒产量的影响. 中国草食动物, 1999, 1(6): 12-13.

Xie B S, Yang Z X, Ma W L, Suonan Y D. Effect of melatonin on the wool growth and production of Menyuan yak in spring. China Herbivores, 1999, 1(6): 12-13. (in Chinese)

[8]杜海燕. 外源褪黑素对羊驼春季绒毛生产性能的影响[D]. 太古: 山西农业大学, 2005.

Du H Y. Effects of exogenous melatonin on performance of alpaca fleece in spring[D]. Taigu: Shanxi Agricultural University, 2005. (in Chinese)

[9]Fischer T W, Burmeister G, Schmidt H W, Elsner P. Melatonin increases anagen hair rate in women with androgenetic alopecia or diffuse alopecia: results of a pilot randomized controlled trial. British Journal of Dermatology, 2004, 150(2): 341-345.

[10]Klören W R L, Norton B W, Waters M J. Fleece growth in Australian Cashmere goats.I. The effects of nutrition and age on fleece growth, prolactin and thyroxine concentration. Australian Journal of Agricultural Research, 1993, 44(5): 1003 -1021.

[11]Martinet L, Allain D, Weiner C. Role of prolactin in the photoperiodic control of moulting in the mink (Mustela visori). Journal of Endocrinology, 1984, 103: 9-15.

[12]贾志海. 褪黑激素促山羊绒生长机理的研究[D]. 北京: 中国农业大学, 1994.

Jia Z H. The mechanism of photoperiod and melatonin on Cashmere growth[D]. Beijing: China Agricultural University, 1994. (in Chinese)

[13]贾志海, 安民. 山羊绒生长机理研究进展. 草食家畜, 1996(1): 2-6.

Jia Z H, An M. Advances in goat hair growth mechanism research. Feeding Livestock, 1996(1): 2-6. (in Chinese)

[14]柳建昌, 尹协镇, 方天祺. 褪黑激素对中国绒山羊在非生绒期促绒生长与绒产量的影响. 动物学杂志, 1998, 33(3): 8-12.

Liu J C, Yin X Z, Fang T Q. Effect of melatonin on the cashmere growth and production of Chinese white goats. Journal of Zoology, 1998, 33(3): 8-12. (in Chinese)

[15]常子丽. 持续埋植褪黑激素对绒山羊绒毛生长特性和相关基因影响的研究[D]. 呼和浩特: 内蒙古农业大学, 2010.

Chang Z L. Study on effect of constant-release melatonin implants on the Cashmere growth traits of Cashmere goats and related gene[D]. Hohhot: Inner Mongolia Agricultural University, 2010. (in Chinese)

[16]Müller-Röver S, Handjiski B, van der Veen C, Eichmüller S, Foitzik K, McKay I A, Stenn K S, Paus R. A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. The Journal of Investigative Dermatology, 2001, 117(1): 3-15.

[17]St-Jacques B, Dassule H R, Karavanova I, Botchkarev V A, Li J, Danielian P S, McMahon J A, Lewis P M, Paus R, McMahon A P. Sonic hedgehog signaling is essential for hair development. Current Biology, 1998, 8(19): 1058-1068.

[18]常子丽. 皮肤毛囊基因调控网络的初建及其在绒山羊上的功能预测[D]. 呼和浩特: 内蒙古农业大学, 2007.

Chang Z L. Primary construction for gene regulatory network of the skin follicle and functional prediction in Cashmere goats[D]. Hohhot: Inner Mongolia Agricultural University, 2007. (in Chinese)

[19]Kamp H, Geilen C C, Sommer C, Blume-Peytavi U. Regulation of PDGF and PDGF receptor in cultured dermal papilla cells and follicular keratinocytes of the human hair follicle. Experimental Dermatology, 2003, 12(5): 662-672.

[20]秦全红, 王德文, 彭瑞云, 谷庆阳, 高亚兵, 夏国伟, 崔雪梅, 刘杰. PDGF-A, PDGFRa在单纯创伤和照射复合创伤愈合过程中伤口成纤维细胞内的表达与意义. 中华放射医学与防护杂志, 2002, 22(6): 401-404.

Qin Q H, Wang D W, Peng R Y, Gu Q Y, Gao Y B, Xia G W, Cui X M, Liu J. Expression of PDGF-A and PDGFR-α in fibroblasts of usual and radiation-combined wound healing. Chinese Journal of Radiological Medicine and Protection, 2001, 22(6): 401-404. (in Chinese)

[21]Pontén F, Ren Z P, Nistér M, Westermark B, Pontén J. Epithelial-stromal interactions in basal cell cancer: the PDGF system. The Journal of Investigative Dermatology, 1994, 102(3): 304-309.

[22]Akiyama M, Smith L T, Holbrook K A. Growth factor and growth factor receptor localization in the hair follicle bulge and associated tissue in human fetus. The Journal of Investigative Dermatology, 1996, 106(3): 391-396.

[23]Goodman L V, Ledbetter S R. Secretion of stromelysin by cultured dermal papilla cells. Differential regulation by growth factors and functional role in mitogen-induced cell proliferation. Journal of Cellular Physiology, 1992, 151(1): 41-49.

[24]Gao J, de Rouen M C, Chen C H, Nguyen M, Nguyen N T, Ido H, Harada K, Sekiguchi K, Morgan B A, Miner J H, Oro A E, Marinkovich M P. Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis. Genes and Development, 2008, 22: 2111-2124.

[25]尹俊. 内蒙古绒山羊毛囊发育、生长周期及相关基因的研究[D]. 呼和浩特: 内蒙古农业大学, 2004.

Yin J. The study of Inner Mongolia goat hair follicle development, growth cycle and related genes[D]. Hohhot: Inner Mongolia Agricultural University, 2004. (in Chinese)

[26]牛一兵, 贾志海, 卢德勋, 郭宝林, 孔祥浩, 王润莲. 放牧条件下绒山羊绒毛季节性生长变化规律的研究. 动物营养学报, 2005, 17(2): 33-37.

Niu Y B, Jia Z H, Lu D X, Guo B L, Kong X H, Wang R L. The study of regular pattern in grazing Cashmere goat hair growth seasonal variation. Chinese Journal of Animal Nutrition, 2005, 17(2): 33-37. (in Chinese)

[27]张春兰. 内蒙古阿尔巴斯白绒山羊毛囊生长周期性变化与KAP6 cDNA的特征分析[D]. 呼和浩特: 内蒙古农业大学, 2003.

Zhang C L. Periodic variety of hair follicles about Inner Mongolia ARBAS White Cashmere goats and characteristic of KAP6 Cdna[D]. Hohhot: Inner Mongolia Agricultural University, 2003. (in Chinese)

[28]岳春旺, 张微, 孔祥浩, 刘海英, 贾志海. 褪黑激素对内蒙古白绒山羊产绒性能的影响. 中国畜牧杂志, 2007, 43(7): 32-34.

Yue C W, Zhang W, Kong X H, Liu H Y, Jia Z H. Melatonin effect the performance of the Cashmere fibre. Chinese Journal of Animal Science, 2007, 43(7): 32-34. (in Chinese)

[29]Moore R W, Bigham M L, Staples L D. Effect of regulin implants on spring fertility, lactation and down growth of Cashmere does. Proceedings of the New Zealand of Animal Production, 1989, 49: 39-42.

[30]Ibraheem M, Galbraith H, Scaife J, Ewen S. Growth of secondary hair follicles of the Cashmere goat in vitro and their response to prolactin and melatonin. Journal of Anatomy, 1994, 185: 135-142.

[31]张燕军. 内蒙古绒山羊胎儿期皮肤毛囊形态发生和发育规律及部分Hox基因家族成员研究[D]. 呼和浩特: 内蒙古农业大学, 2007.

Zhang Y J. Study on skin follicle morphogenesis, development in the fetal period and Hox genes of Inner Mongolian Cashmere goats [D]. Hohhot: Inner Mongolia Agricultural University, 2007. (in Chinese)

[32]王林枫, 卢德勋, 孙海洲, 赵秀英, 珊丹. 光照和褪黑激素对内蒙古绒山羊氮分配和产绒性能的影响. 中国农业科学, 2006, 39(5): 1004-1010.

Wang L F, Lu D X, Sun H Z, Zhao X Y, Shan D. Effects of photoperiod and implanted melatonin on nitrogen partitioning and production performance in Inner Mongolia White Cashmere goats. Scientia Agricultura Sinica, 2006, 39(5): 1004-1010. (in Chinese)