中国农业科学 ›› 2011, Vol. 44 ›› Issue (18): 3903-3908.doi: 10.3864/j.issn.0578-1752.2011.18.022

• 兽医 • 上一篇    下一篇

连翘酯苷对鸡IFN-α和JAK-STAT信号通路相关因子的影响

李华伟,张羽璐,张中文,夏楠,郭玮,吴国娟   

  1. 1.北京农学院动物科学技术学院
  • 收稿日期:2010-11-09 修回日期:2011-03-16 出版日期:2011-09-15 发布日期:2011-05-25
  • 通讯作者: 通信作者吴国娟,E-mail:wgj9288@sina.com。并列通信作者张中文,E-mail:zwzhang9288@ sina.com
  • 作者简介:李华伟,E-mail:54huawei@163.com
  • 基金资助:

    国家自然科学基金(31172362,30771625,30972215)、北京市自然科学基金(6092004)、北京市属高校人才强教计划(PHR2009-07135)

Forsythiaside A Induced IFN-α and Up-Regulated Correlation Factors of JAK-STAT Signaling Pathways in vitro

 LI  Hua-Wei, ZHANG  Yu-Lu, ZHANG  Zhong-Wen, XIA  Nan, GUO  Wei, WU  Guo-Juan   

  1. 1.北京农学院动物科学技术学院
  • Received:2010-11-09 Revised:2011-03-16 Online:2011-09-15 Published:2011-05-25

摘要: 【目的】探讨连翘酯苷作用于鸡胚肾(CEK)细胞后,IFN-α的表达变化及JAK-STAT通路相关因子mRNA的表达变化,及连翘酯苷对IFN-α和JAK-STAT信号通路的影响。【方法】将连翘酯苷设为3个浓度(100、200和400 μg•mL-1),采用荧光定量RT-PCR,检测IFN-α和JAK-STAT通路相关因子的mRNA表达变化;用western blotting检测IFN-α的蛋白表达情况。【结果】与正常组相比,连翘酯苷不仅显著提高了IFN-α的表达量(P<0.05);而且明显上调了STAT1、JAK1、IFNAR1、IFNAR2、IRF1、IRF7的表达。【结论】连翘酯苷能够在鸡胚肾细胞(chicken embryo kidney cells,CEKs) 上诱导IFN-α的表达,且能够正向调节JAK-STAT信号通路传导。

关键词: 连翘酯苷, 鸡胚肾细胞, IFN-α, JAK-STAT, real-timePCR, westernblotting

Abstract: 【Objective】 This study focused on the effect of forsythiaside A added in chick embryo kidney cells, the expression of IFN-αand the mRNA expression of correlation factors in JAK-STAT pathway. 【Methods】 In the study, three levels forsythiaside (100, 200, 400 μg•mL-1) were set, and real-time RT-PCR was used to detect the mRNA expression of IFN-α and JAK-STAT pathway related factors; western blotting was used to investigate protein expression of IFN-α. 【Result】 Compared with normal group, the expression of IFN-α increased significantly when high dose forsythiaside A was added (P<0.05). And the expression of STAT1, JAK1, IFNAR1, IFNAR2, IRF1, IRF7 was significantly increased either. 【Conclusion】Forsythiaside A induced the expression of IFN-α in chicken embryo kidney cells (CEKs), and can positively regulate the JAK-STAT signaling pathway significantly.

Key words: forsythosideA, CEKcell, IFN-α, JAK-STAT, real-timePCR, westernblotting