Abstract:

【Objective】The objective of the experiment is to develop a SCAR(sequence characterized amplified region) marker of Fusarium wilt resistant gene FOC-1 in cabbage.【Method】Bulked segregation analysis (BSA) DNA pools were constructed by F2 individuals derived from the cross between resistant ‘8024’× susceptible ‘6A’ cross. An AFLP marker genetically linked to the Fusarium wilt resistant gene was identified and it was converted into a more valuable and simple SCAR marker S46M48199 consequently. Genetic linkage between S46M48199 and FOC-1 was analyzed by using 142 F2 progenies. The validity and practicality of S46M48199 was verified in two different cabbage F2 populations F66 and C1.【Result】One stable SCAR marker S46M48199 linked in repulsion to the dominant resistant allele at 2.78 cM from the FOC-1 locus was developed successfully. The validity and practicality of S46M48199 in two other populations revealed significant consistency with phenotype assay (81% and 83%, respectively). 【Conclusion】The SCAR marker developed in this study could be used for MAS of Fusarium wilt resistance in cabbage.