Abstract:

【Objective】The objective of this study is to isolate and identify bacterial community and enzyme-producing bacteria in intestine of Antheraea pernyi larvae and to develop microecological agents for increasing leaf-silk conversation rate and disease resistance. 【Method】Bacteria were isolated from intestine of fifth instars Antheraea pernyi larvae reared on oak leaves by isolated culture. Intestinal bacterial community was identified according to physiological and biochemical characteristics and phylogenetic analysis based on 16S rDNA sequences. Cellulase, protease, lipase-producing strains were screened on selective medium. 【Result】The intestinal bacteria isolated from Antheraea pernyi larvae belong to Bacillus, Staphylococcus and Enterobacter. Among them, Bacillus is the main bacteria and the main enzyme-producing bacteria which could produce cellulase and protease, Staphylococcus could produce protease weakly, Enterobacter couldn’t produce enzyme.【Conclusion】Intestinal bacteria community of Antheraea pernyi was similar to that of Bombyx mori, which could be developed as microecological agents in sericulture for the enzyme-producing strains exhibiting high activity.