异噁草酮,降解菌Y1,降解率," /> 异噁草酮,降解菌Y1,降解率,"/> clomazone,degrading bacteria Y1,degradation rate
,"/> <font face="Verdana">影响降解菌Y1降解土壤中异噁草酮的三种因素的优化#br# </font>

中国农业科学 ›› 2010, Vol. 43 ›› Issue (12): 2453-2459 .doi: 10.3864/j.issn.0578-1752.2010.12.008

• 植物保护 • 上一篇    下一篇

影响降解菌Y1降解土壤中异噁草酮的三种因素的优化#br#

刘亚光,李洁,唐广顺   

  1. (东北农业大学农学院)
  • 收稿日期:2009-10-23 修回日期:2010-03-17 出版日期:2010-06-15 发布日期:2010-06-15

Optimization of Three Factors Affecting Clomazone Degradation in Contaminated Soil by Degrading Bacteria Y1 #br#

LIU Ya-guang, LI Jie, TANG Guang-shun#br#   

  1. (东北农业大学农学院)
  • Received:2009-10-23 Revised:2010-03-17 Online:2010-06-15 Published:2010-06-15

摘要:

【目的】研究田间持水量、接种量以及肥料添加量对降解菌Y1降解土壤中异噁草酮的影响,优化异噁草酮降解菌Y1的降解条件。【方法】利用生物测定的方法,通过二次回归旋转组合设计确定最优降解条件。【结果】得到降解条件的优化数学回归模型为y=65.05812+9.60065*C1+10.49792*C2+3.60229*C3-6.88851*C12-4.41187*C22。各因素对降解率的影响大小顺序依次为接种量、田间持水量、肥料添加量。【结论】当接种量(13.46—15.48 mL&#8226;kg-1),田间持水量(56.93%—62.0%),肥料添加量(1.82—2.18 g&#8226;kg-1)分别在所示范围内时,降解菌在异噁草酮有效成分浓度为500 µg&#8226;kg-1的风干土壤中,30 d后的降解率可达到60%以上。

关键词: 异噁草酮')">异噁草酮, 降解菌Y1, 降解率

Abstract:

【Objective】 For optimization of clomazone degradation in contaminated soil by degrading bacteria Y1, three factors affecting degradation rate including field moisture capacity, the amount of inoculum and fertilizer used were studied. 【Method】 Based on the bioassay method , the degradation rates of bacteria Y1 under different conditions were compared by using orthogonally rotational combination design. 【Result】 A mathematical regression model for clomazone degradation was established, that is, y= 65.05812+9.60065*C1+10.49792*C2+3.60229*C3-6.88851*C12-4.41187*C22. Results indicated that the order of significance affecting degradation rate is: the amount of inoculum>the field moisture capacity>the amount of fertilize used.【Conclusion】When the amount of inoculum was 13.46—15.48 mL&#8226;kg-1, the field moisture capacity was 56.93%—62.0% and the amount of fertilize used was 1.82—2.18 g&#8226;kg-1, the degradation rate was over 60% in air-dried soil contained clomazone 500 µg&#8226;kg-1after 30 days.

Key words: clomazone')">clomazone, degrading bacteria Y1, degradation rate