关键词: 同安钮夜蛾核型多角体病毒')">同安钮夜蛾核型多角体病毒, odv-e66 基因, odv-ec43基因, ac108基因, p87/vp80 基因

Abstract:

【Objective】 In order to investigate the genomic organization and phylogenetic status of OpdiNPV. 【Method】OpdiNPV DNA was digested with pstⅠ and separated by electrophoresis. A pst I-G fragment was recovered, ligated into PUC18 vector, and transformed into E. coli strain DH5α. Recombinant plasmids were purified from white bacterial colonies and sequenced.【Result】 The pst I-G fragment was 5056bp in length, containing four open reading frames (ORFs) that encode odv-e66 gene (EU 623602), p87/vp80 gene (EU 732665), odv-ec43 gene (EU617337) and ac108 gene (EU 732666) respectively. A late promoter motif TAAG was found 12 nucleotides (nt) upstream of the start codon ATG of ac108 gene. Upstream of the start codon ATG of odv-ec43 has two early transcription motifs CAGT and a late promoter motif TAAG. Two transmembrane helices, 3 N-glycosylation sites, 1 N-myristoylation site, 7 Protein kinase C phosphorylation sites, 1 Tyrosine kinase phosphorylation site, 4 Casein kinase Ⅱ phosphorylation sites were found in the protein deduced by odv-ec43.A polyadenylation signal site was found 89 nucleotides (nt) downstream of the stop codon TAA of p87/vp80 gene. 【Conclusion】 Amino acid sequence alignment and identity analysis showed that Ac108, ODV-E66 and P87/VP80 had a low identity with other baculovirus. ODV-Ec43 was conserved among baculoviruses.

Key words: Ophiusa disjungens nucleopolyhedrovirus')">Ophiusa disjungens nucleopolyhedrovirus, odv-e66 gene, odv-ec43 gene, ac108 gene, p87/vp80 gene