中国农业科学 ›› 2008, Vol. 41 ›› Issue (7): 2149-2153 .doi: 10.3864/j.issn.0578-1752.2008.07.037

• 兽医 • 上一篇    下一篇

脂多糖刺激对断奶仔猪脾脏、胸腺和外周血白细胞PPARγ蛋白表达的影响

鲁 晶,刘玉兰,石君霞,侯永清,刘洪明,朱惠玲,丁斌鹰,郭广伦   

  1. 武汉工业学院饲料科学系/动物营养与饲料科学湖北省重点实验室
  • 收稿日期:2007-05-24 修回日期:2007-08-22 出版日期:2008-07-10 发布日期:2008-07-10
  • 通讯作者: 刘玉兰

The Effect of LPS Challenge on PPARγ Protein Expression in Immune Organs and Immune Cells of Weaned Pigs

Jing LU   

  1. 武汉工业学院饲料科学系/动物营养与饲料科学湖北省重点实验室
  • Received:2007-05-24 Revised:2007-08-22 Online:2008-07-10 Published:2008-07-10

摘要: 【目的】研究免疫应激对断奶仔猪脾脏、胸腺和外周血白细胞过氧化物酶体增殖物活化受体γ(PPARγ)蛋白表达水平的影响。【方法】选取12头健康断奶仔猪,分成2组,每组6个重复。试验组注射100 μg•kg-1 BW的脂多糖(LPS)建立免疫应激模型,对照组注射等量生理盐水。注射后3 h,采血分离血浆和白细胞待测,然后立即屠宰仔猪,取脾脏和胸腺,通过Western blot 测定脾脏、胸腺和白细胞中PPARγ蛋白表达水平。【结果】LPS刺激仔猪,导致血浆白细胞介素-6﹑肿瘤坏死因子-α、皮质醇和前列腺素E2含量显著升高(P<0.05),胰岛素、类胰岛素生长因子-1含量显著降低(P<0.05);LPS刺激导致血浆葡萄糖、总蛋白、白蛋白和球蛋白含量显著降低(P<0.05);LPS刺激导致脾脏(P<0.05)、胸腺(P<0.1)和白细胞(P<0.05)PPARγ蛋白表达水平升高。【结论】脂多糖剌激可提高仔猪脾脏、胸腺和外周血白细胞PPARγ蛋白的表达,显示PPARγ可能参与仔猪免疫应激的调控,可能成为缓解仔猪免疫应激的一个新靶点。

关键词: 断奶仔猪, 脂多糖, PPARγ

Abstract: 【Objective】An experiment was conducted to investigate the effect of lipopolysaccharide (LPS) challenge on peroxisome proliferator-activated receptor γ (PPARγ) protein expression in immune organs and immune cells of weaned pigs. 【Method】Pigs were allotted to two treatments (6 replicates/treatment and 1 pig/replicate). The pigs in the treatment group were injected intraperitoneally with 100μg/kg BW LPS, whereas pigs in the control group were injected with an equivalent amount of sterile saline. Blood samples were collected at 3h post-challenge for isolating plasma or leucocytes. Following blood collection, the pigs were slaughtered to collect spleens, thymus and intestinal lymph nodes. PPARγ protein expression of immune organs and the leucocytes were determined by Western Blot. 【Result】(1) LPS challenge increased significantly plasma interleukin-6, tumor necrosis factor-α, cortisol and Prostaglandin E2 (P<0.05), and decreased insulin and insulin-like growth factor-1 (P<0.05); (2) LPS challenge decreased significantly plasma glucose, total protein, albumin and globulin (P<0.05); (3) LPS challenge increased significantly PPARγ protein expression of spleens (P<0.05), thymus (P<0.1) and leucocytes (P<0.05), and decreased PPARγ protein expression of intestinal lymph nodes (P<0.05).【Conclusion】Immunological stress changes PPARγ protein expression in immune organs and immune cells of weaned pigs, which indicates PPARγ plays an important role in regulation of immunological stress, and may prove to be an important target of alleviating the immunological stress in pigs.

Key words: Weaned pigs, LPS, PPARγ