关键词: 萨能山羊, 乳腺组织, Ubi-d4基因, 抑制性消减杂交

Abstract: Ubi-d4, belonging to d4 gene family, it involves in apoptosis response and modulates cell growth and turnover. The object of this study was to identify and verify if Ubi-d4 was involved in mammary gland involution of goat. Mammary gland samples came from Xinong Saanen goat at middle and late lactation periods. Suppression subtractive hybridization (SSH) approach, reverse transcriptase-polymerase chain reaction (RT-PCR), real-time reverse transcriptase-polymerase chain reaction (RT-PCR), and biotechnology were employed to study Ubi-d4. Results: Goat mammary gland zinc-finger protein Ubi-d4 had an open reading frame of 1176 nucleotides encoding a putative protein of 391 amino acid, the GenBank No was EF105410), the homologies of nucleotide and peptide sequence of this Ubi-d4 with bovine (XM_881516), human (NM_006268.3), mouse’s (NM_011262) and rabbit (XM_341998) were 98％, 90％, 93％, 90％ and 99％, 99％, 99％,98％ respectively; the amino acid sequence of Ubi-d4 included one C2H2 type zinc-finger, one PHD-type zinc-finger, and had no signal peptide, no transmember structure. Conclusion: we successfully identified goat zinc-finger protein Ubi-d4 gene and predicted its function which might give valuable information for further functional studying.

Key words: Xinong saanen goat, Mammary gland, Ubi-d4, Suppression subtractive hybridization