骨保护素对体外培养大鼠破骨细胞的影响

doi:10.3864/j.issn.0578-1752.2008.02.037

中国农业科学 ›› 2008, Vol. 41 ›› Issue (2): 581-586 .doi: 10.3864/j.issn.0578-1752.2008.02.037

骨保护素对体外培养大鼠破骨细胞的影响

刘俊栋,顾建红,翟必华,刘学忠,卞建春,刘宗平

扬州大学兽医学院

收稿日期:2006-10-10 修回日期:2007-03-26 出版日期:2008-02-10 发布日期:2008-02-10
通讯作者: 刘宗平

Effect of osteoprotegerin on rat's osteoclasts formation and activation in vitro

扬州大学兽医学院

Received:2006-10-10 Revised:2007-03-26 Online:2008-02-10 Published:2008-02-10

摘要/Abstract

摘要： 【目的】探讨不同浓度骨保护素（osteoprotegerin，OPG）对破骨细胞（osteoclasts，OC）生成和活化的影响。【方法】通过成骨细胞（osteoblasts，OB）与脾细胞共培养的方法在体外诱导脾细胞转化为OC。采用形态学观察、抗酒石酸酸性磷酸酶（TRAP）染色计数、扫描电镜观察象牙片吸收陷窝方法检测OC的生成和活化。【结果】随着OPG浓度（5、10、25、50、75 ng•ml-1）的增加，TRAP阳性OC数逐渐减少，各试验组与对照组比较差异均极显著（P＜0.01）；象牙片吸收陷窝的数目和面积也逐渐减少，OPG浓度为50 ng•ml-1、75 ng•ml-1时观测不到吸收陷窝。【结论】OPG通过抑制OC生成和活化，从而抑制OC的骨吸收，OPG浓度达50 ng•ml-1以上可完全阻断OC的活化。

关键词: 破骨细胞, 骨保护素, 抗酒石酸酸性磷酸酶, 吸收陷窝

Abstract: Abstract: 【OBJECTIVE】To study the effect of different concentration of OPG on osteoclasts. 【METHOD】Osteoclasts were obtained by a co-culture system with rat spleen cells and osteoblasts, and supplemented with 5, 10, 25, 50 or 75 ng/ml of OPG respectively. The formation and activation of osteoclasts were ditected by ways of morphologic observation, histochemical staining for tartrate-resistant acid phosphatase (TRAP) and detection of lacunar resorption through scanning electron micrograph. 【RESULTS】Compared with the control group, the number of TRAP positive osteoclasts in experiment group decreased very significantly (P＜0.01). The number and area of lacunar resorption decreased with concentration increasing of OPG, withing the decreasing of OPG. Lacunar resorption wasn’t detected in the group, witch with more than 50 ng/ml OPG. 【CONCLUSION】The resorption activity of osteoclasts is inhibited by inducing osteoclasts formation and activation under OPG.

Key words: osteoclast, osteoprotegerin, tartrate-resistant acid phosphatase, lacunar resorption