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1. The impacts of oxytetracycline on humification during manure composting can be alleviated by adjusting initial moisture contents as illustrated by NMR
FENG Yao, WANG Gui-zhen, LIU Yuan-wang, CHENG Deng-miao, FAN Shuang-hu, ZHAO Quan-sheng, Jianming XUE, ZHANG Shu-qing, LI Zhao-jun
Journal of Integrative Agriculture    2021, 20 (8): 2277-2288.   DOI: 10.1016/S2095-3119(20)63332-9
摘要124)      PDF    收藏

土霉素广泛存在于土壤和水体中,可能对农业生态环境构成潜在威胁。含土霉素的粪便作为有机肥施用是土霉素进入土壤和水体的主要途径之一。因此,实现粪便堆肥过程中土霉素的有效去除,并明确土霉素存在下堆肥有机质的转化规律十分重要。本文通过调节不同初始含水率(45%、55%、65%),外源模拟添加土霉素,将鸡粪和小麦秸秆共堆肥,以研究堆肥过程中土霉素降解及有机质变化规律。研究结果表明,堆肥初始含水率对土霉素降解有显著影响,与较低初始含水率相比,初始含水率为65%更有利于土霉素降解(降解率77.4%)和堆肥腐熟,然而,高初始含水率(65%)可能促进堆肥过程的硝化作用,从而增加了氨挥发。此外,增加堆肥初始含水率可以提高堆肥温度。核磁共振结果显示,土霉素的存在可以影响脂肪类物质的内部转化,从而抑制堆肥的腐殖化。55-65%的初始含水率可以缓解粪便堆肥过程中土霉素对腐殖化的影响。


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2. Microbial community dynamics during composting of animal manures contaminated with arsenic, copper, and oxytetracycline
Ebrahim SHEHATA, CHENG Deng-miao, MA Qian-qian, LI Yan-li, LIU Yuan-wang, FENG Yao, JI Zhen-yu, LI Zhao-jun
Journal of Integrative Agriculture    2021, 20 (6): 1649-1659.   DOI: 10.1016/S2095-3119(20)63290-7
摘要170)      PDF    收藏
Effects of the heavy metal copper (Cu), the metalloid arsenic (As), and the antibiotic oxytetracycline (OTC) on bacterial community structure and diversity during cow and pig manure composting were investigated.  Eight treatments were applied, four to each manure type, namely cow manure with: (1) no additives (control), (2) addition of heavy metal and metalloid, (3) addition of OTC and (4) addition of OTC with heavy metal and metalloid; and pig manure with: (5) no additives (control), (6) addition of heavy metal and metalloid, (7) addition of OTC and (8) addition of OTC with heavy metal and metalloid.  After 35 days of composting, according to the alpha diversity indices, the combination treatment (OTC with heavy metal and metalloid) in pig manure was less harmful to microbial diversity than the control or heavy metal and metalloid treatments.  In cow manure, the treatment with heavy metal and metalloid was the most harmful to the microbial community, followed by the combination and OTC treatments.  The OTC and combination treatments had negative effects on the relative abundance of microbes in cow manure composts.  The dominant phyla in both manure composts included Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria.  The microbial diversity relative abundance transformation was dependent on the composting time.  Redundancy analysis (RDA) revealed that environmental parameters had the most influence on the bacterial communities.  In conclusion, the composting process is the most sustainable technology for reducing heavy metal and metalloid impacts and antibiotic contamination in cow and pig manure.  The physicochemical property variations in the manures had a significant effect on the microbial community during the composting process.  This study provides an improved understanding of bacterial community composition and its changes during the composting process. 
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3.
The effects of calcium combined with chitosan amendment on the bioavailability of exogenous Pb in calcareous soil
CHANG Hui-qing, WANG Qi-zhen, LI Zhao-jun, WU Jie, XU Xiao-feng, SHI Zhao-yong
Journal of Integrative Agriculture    2020, 19 (5): 1375-1386.   DOI: 10.1016/S2095-3119(19)62861-3
摘要129)      PDF    收藏
Lead (Pb) in soil may accumulate in crops and enter the human body.  This study aimed to understand the speciation transformation and accumulation characteristics of exogenous Pb in calcareous soil with or without the application of soil amendments.  Field experiments with continuous maize cultivation have been carried out for two years.  The results showed that the contents of total Pb were slightly lower in 2016 than in 2015 for the same treatments; however, no significant difference between the years was observed.  Soil Pb existed mainly in the residual fraction without exogenous Pb addition, and its proportion was more than 33% of the total soil Pb in the control and Ca treatments.  When Pb was added to calcareous soil, Pb existed largely in the oxidizable and reducible fractions during the two-year experimental period, and those fractions made up over 83% of the total Pb.  The proportion of the water-soluble Pb, regardless of the addition of Pb, was the lowest and was less than 0.0019% in all treatments, but the addition of Ca and chitosan amendments reduced the water-soluble and exchangeable Pb contents.  The Pb content in different parts of maize followed the order root>leaf>stem>grain during the experimental period.  Although maize had low transfer and enrichment factors in calcareous soil, which make the Pb content in the grain show no significant difference among the five treatments in the same year, adding Ca and chitosan to calcareous soil can reduce the Pb contents of maize, especially reduce the Pb contents of root, stem and leaf.  Therefore, the addition of calcium and chitosan is an effective strategy for reducing Pb availability in calcareous soils.
 
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4. Screening and degradation characteristics of a tylosin-degrading strain
FENG Chang-qing, CHENG Deng-miao, FENG Yao, QI Wei-ning, JIA Zhen-hu, Louise WEAVER, LIU Yuan-wang, LI Zhao-jun
Journal of Integrative Agriculture    2020, 19 (4): 1127-1136.   DOI: 10.1016/S2095-3119(19)62764-4
摘要159)      PDF    收藏
Antibiotics residues have been accumulating in the environment day by day due to overuse of antibiotics.  Recalcitrant antibiotic residues, such as tylosin (TYL), can cause serious environmental problems, which makes it important to eliminate TYL from the environment.  It is important to eliminate TYL from the environment.  In this study, a strain was isolated and purified from fermentation by-product that came from a TYL production factory.  The TYL degrading strain was identified by its morphology, physiological and biochemical reactions and sequencing the PCR-amplified fragments of its 16S rDNA-coding genes.  The temperature, shaking speed, initial TYL concentration, pH and inoculum sizes were investigated under simulated conditions by using single factor tests.  The results showed that TYL2, a high efficient strain was isolated and was identified as Brevibacillus borstelensis.  The degradation rate of TYL by this strain could reach to 75% with an initial concentration of 25 mg L–1 within 7 days under conditions of 7% B. borstelensis (v/v, 2×108 CFU mL–1) at pH 7.0 and at 35°C.  It is interesting that this strain has a very strong ability to degrade the TYL in natural sewage with the degradation rate of 65% within 7 days.  This result could be helpful for the degradation of TYL and provide guidance for the degradation of other antibiotics.
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5. Degradation mechanisms of oxytetracycline in the environment
LI Zhao-jun, QI Wei-ning, FENG Yao, LIU Yuan-wang, Ebrahim Shehata, LONG Jian
Journal of Integrative Agriculture    2019, 18 (9): 1953-1960.   DOI: 10.1016/S2095-3119(18)62121-5
摘要132)      PDF    收藏
Over the past few decades, the usage of oxytetracycline (OTC), a kind of antibiotic, has increased with the development of aquaculture and livestock breeding.  However, about 30–90% of the applied antibiotics are excreted as the parent compounds into the environment, especially with the application of animal manure to agricultural fields.  This large influx of antibiotics may lead to the destruction of the natural microbial ecological community and pose great threats to human beings through the food chain.  Therefore, the fate and toxicity of OTC in the environment are issues of great concern.  Degradation of OTC, including the non-biodegradation and biodegradation, and the biological toxicity of its degradation products or metabolites, are reviewed in this paper.  The non-biodegradation pathways include hydroxylation, quinonization, demethylation, decarbonylation, dehydration and secondary alcohol oxidation.  Light (particularly UV light), pH and oxidizing substances play important roles in non-biodegradation.  Biodegradation products include 4-epi-OTC (EOTC), 2-acetyl-2-decarboxy-amido-OTC (ADOTC), α-apo-OTC and β-apo-OTC.  EOTC is an epimer and identied except for the configuration of the C4 dimethylamino group of OTC.  Temperature and pH are the main factors affecting biodegradation pathways of OTC.  In addition, this review discusses concerns over the biological toxicity of OTC degradation products.
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6. Removal of Cd and Pb with biochar made from dairy manure at low temperature
CHEN Zhi-liang, ZHANG Jian-qiang, HUANG Ling, YUAN Zhi-hui, LI Zhao-jun, LIU Min-chao
Journal of Integrative Agriculture    2019, 18 (1): 201-210.   DOI: 10.1016/S2095-3119(18)61987-2
摘要286)      PDF    收藏

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7. Uptake and translocation of organic pollutants in plants: A review
ZHANG Cheng, FENG Yao, LIU Yuan-wang, CHANG Hui-qing, LI Zhao-jun, XUE Jian-ming
Journal of Integrative Agriculture    2017, 16 (08): 1659-1668.   DOI: 10.1016/S2095-3119(16)61590-3
摘要856)      PDF    收藏
    Organic pollutants, such as polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs), antibiotics, herbicides, and bisphenol A (BPA), are commonly found in agricultural environments. They are released into the environment as a result of their use for human health purposes and farm management activities, and are often discharged as waste-water effluents. Most of these organic pollutants are taken up by plants through roots and leaves, and when they enter the tissue, they cause serious damage to the plants. Although the toxicity of organic pollutants to plants, especially to plant cells, has been intensively studied, a systematic review of these studies is lacking. Here we review researches on the toxicity of organic pollutants, their uptake, and translocation in plants. Our objective is to assemble existing knowledge concerning the interaction of organic pollutants with plants, which should be useful for the development of plant-based systems for removing pollutants from aquatic and agricultural environments.
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8. Prediction model for mercury transfer from soil to corn grain and its cross-species extrapolation
HU Hai-yan, LI Zhao-jun, FENG Yao, LIU Yuan-wang, XUE Jian-ming, Murray Davis, LIANG Yong-chao
Journal of Integrative Agriculture    2016, 15 (10): 2393-2402.   DOI: 10.1016/S2095-3119(15)61261-8
摘要1609)      PDF    收藏
    In this study the transfer characteristics of mercury (Hg) from a wide range of Chinese soils to corn grain (cultivar Zhengdan 958) were investigated. Prediction models were developed for determining the Hg bioconcentration factor (BCF) of Zhengdan 958 from soil, including the soil properties, such as pH, organic matter (OM) concentration, cation exchange capacity (CEC), total nitrogen concentration (TN), total phosphorus concentration (TP), total potassium concentration (TK), and total Hg concentration (THg), using multiple stepwise regression analysis. These prediction models were applied to other non-model corn cultivars using a cross-species extrapolation approach. The results indicated that the soil pH was the most important factor associated with the transfer of Hg from soil to corn grain. Hg bioaccumulation in corn grain increased with the decreasing pH. No significant differences were found between two prediction models derived from different rates of Hg applied to the soil as HgCl2. The prediction models established in this study can be applied to other non-model corn cultivars and are useful for predicting Hg bioconcentration in corn grain and assessing the ecological risk of Hg in different soils.
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9. Negative Effects of Oxytetracycline on Wheat (Triticum aestivum L.) Growth, Root Activity, Photosynthesis, and Chlorophyll Contents  
LI Zhao-jun, XIE Xiao-yu, ZHANG Shu-qing , LIANG Yong-chao
Journal of Integrative Agriculture    2011, 10 (10): 1545-1553.   DOI: 10.1016/S1671-2927(11)60150-8
摘要2097)      PDF    收藏
A solution culture experiment was performed to investigate the effects of oxytetracycline (OTC) on wheat (Triticum aestivum L.) growth, chlorophyll contents, and photosynthesis at five levels of 0, 10, 20, 40, and 80 mmol L-1 OTC. OTC is toxic to wheat. The wheat growth, especially wheat root was significantly decreased. Further OTC also significantly decreased root activity, chlorophyll contents, and photosynthetic parameters except for intercellular CO2 concentrations. The different responses of indicators such as root number, root activity and so on to OTC were also observed. The IC50 values for the tested indicators to OTC ranged from 7.1 to 113.4 mmol L-1 OTC. The order of indicator sensitivity to OTC was root number > stomatal conductance > chlorophyll a > total chlorophyll > photosynthetic rates > total surface area > transpiration rate > chlorophyll b > fresh weight of root > dry weight of root > total length > dry weight of shoot = fresh weight of shoot > total volume. The root number was more sensitive than other indicators with the IC50 value of 7.1 mmol L-1 OTC, and could be taken as the sensitive indicator to predict the hazards of OTC to wheat.
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