Negative Effects of Oxytetracycline on Wheat (Triticum aestivum L.) Growth, Root Activity, Photosynthesis, and Chlorophyll Contents

doi:10.1016/S1671-2927(11)60150-8

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (10): 1545-1553.DOI: 10.1016/S1671-2927(11)60150-8

Negative Effects of Oxytetracycline on Wheat (Triticum aestivum L.) Growth, Root Activity, Photosynthesis, and Chlorophyll Contents

LI Zhao-jun, XIE Xiao-yu, ZHANG Shu-qing , LIANG Yong-chao

1.Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Nutrition and Fertilization, Ministry of Agriculture

收稿日期:2010-10-18 出版日期:2011-10-01 发布日期:2011-10-18
通讯作者: Correspondence LIANG Yong-chao, Professor, Tel:+86-10-82108657, Fax: +86-10-82105161, E-mail: ycliang@caas.ac.cn
作者简介:LI Zhao-jun, Ph D, Associate Professor, Tel: +86-10-82108657, Fax: +86-10-82105161, E-mail: zjli@caas.ac.cn
基金资助:
This research was jointly supported by the National Natural Science Foundation of China (40701163), the Natural Science Foundation of Beijing City, China (6092019), the International Foundation for Science, Stockholm, Sweden, the Organization for the Prohibition of Chemical Weapons, the Hague, and the Netherlands, through a grant to Li Zhaojun (C/4076), and the National Basic Research Program of China (973 Program, 2007CB109305).

Negative Effects of Oxytetracycline on Wheat (Triticum aestivum L.) Growth, Root Activity, Photosynthesis, and Chlorophyll Contents

LI Zhao-jun, XIE Xiao-yu, ZHANG Shu-qing , LIANG Yong-chao

1.Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Nutrition and Fertilization, Ministry of Agriculture

Received:2010-10-18 Online:2011-10-01 Published:2011-10-18
Contact: Correspondence LIANG Yong-chao, Professor, Tel:+86-10-82108657, Fax: +86-10-82105161, E-mail: ycliang@caas.ac.cn
About author:LI Zhao-jun, Ph D, Associate Professor, Tel: +86-10-82108657, Fax: +86-10-82105161, E-mail: zjli@caas.ac.cn
Supported by:
This research was jointly supported by the National Natural Science Foundation of China (40701163), the Natural Science Foundation of Beijing City, China (6092019), the International Foundation for Science, Stockholm, Sweden, the Organization for the Prohibition of Chemical Weapons, the Hague, and the Netherlands, through a grant to Li Zhaojun (C/4076), and the National Basic Research Program of China (973 Program, 2007CB109305).

摘要/Abstract

摘要： A solution culture experiment was performed to investigate the effects of oxytetracycline (OTC) on wheat (Triticum aestivum L.) growth, chlorophyll contents, and photosynthesis at five levels of 0, 10, 20, 40, and 80 mmol L-1 OTC. OTC is toxic to wheat. The wheat growth, especially wheat root was significantly decreased. Further OTC also significantly decreased root activity, chlorophyll contents, and photosynthetic parameters except for intercellular CO2 concentrations. The different responses of indicators such as root number, root activity and so on to OTC were also observed. The IC50 values for the tested indicators to OTC ranged from 7.1 to 113.4 mmol L-1 OTC. The order of indicator sensitivity to OTC was root number > stomatal conductance > chlorophyll a > total chlorophyll > photosynthetic rates > total surface area > transpiration rate > chlorophyll b > fresh weight of root > dry weight of root > total length > dry weight of shoot = fresh weight of shoot > total volume. The root number was more sensitive than other indicators with the IC50 value of 7.1 mmol L-1 OTC, and could be taken as the sensitive indicator to predict the hazards of OTC to wheat.

关键词: OTC, wheat, growth, root morphological characteristics, chlorophyll contents, photosynthesis

Abstract: A solution culture experiment was performed to investigate the effects of oxytetracycline (OTC) on wheat (Triticum aestivum L.) growth, chlorophyll contents, and photosynthesis at five levels of 0, 10, 20, 40, and 80 mmol L-1 OTC. OTC is toxic to wheat. The wheat growth, especially wheat root was significantly decreased. Further OTC also significantly decreased root activity, chlorophyll contents, and photosynthetic parameters except for intercellular CO2 concentrations. The different responses of indicators such as root number, root activity and so on to OTC were also observed. The IC50 values for the tested indicators to OTC ranged from 7.1 to 113.4 mmol L-1 OTC. The order of indicator sensitivity to OTC was root number > stomatal conductance > chlorophyll a > total chlorophyll > photosynthetic rates > total surface area > transpiration rate > chlorophyll b > fresh weight of root > dry weight of root > total length > dry weight of shoot = fresh weight of shoot > total volume. The root number was more sensitive than other indicators with the IC50 value of 7.1 mmol L-1 OTC, and could be taken as the sensitive indicator to predict the hazards of OTC to wheat.

Key words: OTC, wheat, growth, root morphological characteristics, chlorophyll contents, photosynthesis

LI Zhao-jun, XIE Xiao-yu, ZHANG Shu-qing , LIANG Yong-chao. Negative Effects of Oxytetracycline on Wheat (Triticum aestivum L.) Growth, Root Activity, Photosynthesis, and Chlorophyll Contents [J]. Journal of Integrative Agriculture, 2011, 10(10): 1545-1553.

参考文献

[1]Backhaus T, Grimme L H. 1999. The toxicity of antibiotics agents to the luminescent bacterium Vibrio fischeri. Chemosphere, 38, 3291-3301.

[2]Batchelder A R. 1982. Chlortetracycline and oxytetracycline effects on plant growth and development in soil systems. Journal of Environmental Quality, 11, 675-678.

[3]Chopra I, Roberts M. 2001. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiology and Molecular Biology Reviews, 65, 232-260.

[4]Comb J I, Long S I, Scurlock J. 1985. Techniques in Bioproductivity and Photosynthesis. Pergamon Press, Oxford, New York, Toronto, Sydney, Frankfurt. Drillia P, Stamatelatou K, Lyberatos G. 2005. Fate and mobility of pharmaceuticals in solid matrices. Chemosphere, 60, 1034- 1044.

[5]Eguchi K, Nagase H, Ozawa M, Endoh Y S, Goto K, Hirata K, Miyamoto K, Yoshimura H. 2004. Evaluation of antimicrobial agents for veterinary use in the ecotoxicity test using microalgae. Chemosphere, 57, 1733-1738.

[6]Halling-Sørensen B, Sengeløv G, Tjørnelund J. 2002. Toxicity of tetracyclines and tetracycline degradation products to environmentally relevant bacterial including selected tetracycline resistant bacterial. Archives of Environmental Contamination and Toxicology, 42, 263-271.

[7]Hamscher G, Sczesny S, Höper H, Nau H. 2002. Determination of persistent tetracycline residues in soil fertilized with liquid manure by high performance liquid chromatography with electrospray ionization tandem mass spectrometry. Analytical Chemistry, 74, 1509-1518.

[8]Heberer T, Schmidt-Baumler K, Stan H J. 1998. Occurrence and distribution of organic contaminants in the aquatic system in Berlin. Part 1: Drug residues and other contaminants in Berlin surface and groundwater. Acta Hydrochimica ET Hydrobiologica, 26, 272-278.

[9]Hirsch R, Ternes T, Haberer K, Kratz K L. 1999. Occurrence of antibiotics in the aquatic environments. The Science of the Total Environment, 225, 109-118.

[10]Isidori M, Lavorgna M, Nardelli A, Pascarella L, Parrella A. 2005. Toxic and genotoxic evaluation of six antibiotics on non-target organisms. The Science of the Total Environment, 345, 87-98.

[11]Jjemba P K. 2002. The effect of chloroquine, quinacrine, and metronidazole on both soybean plants and soil microbiota. Chemosphere, 46, 1019-1025.

[12]Kolpin D W, Furlong E T, Meyer M T, Thurman E M, Zaugg S D, Barber L R, Buxton H T. 2002. Pharmaceuticals, hormones, and other organic wastewater contaminants in U. S. streams, 1999-2000: a national reconnaissance. Environmental Science & Technology, 36, 1202-1211.

[13]Li Z J, Xu J M, Ma G R, Muhammad A. 2007. Changes in ALS, SOD, POD, and CAT activity in rice plants stressed by bound residues of metsulfuron-methyl in soil. Pedosphere, 17, 487-492.

[14]Li Z J, Xu J M, Muhammad A, Ma G R. 2005. Effect of bound residues of metsulfuron-methyl in soil on rice growth. Chemosphere, 58, 1177-1183.

[15]Lindström A, Nyström C. 1987. Seasonal variation in root hardiness in container grown Scots pine, Norway spruce, and Lodgepole pine seedlings. Canadian Journal of Forest Research, 17, 787-793.

[16]Loke M L, Tjømelund J, Halling-Sørensen B. 2002. Determination of the distribution coefficient (log Kd) of oxytetracycline, tylosin A, olaquindox and metronidazole in manure. Chemosphere, 48, 351-361.

[17]Migliore L, Civitareale C, Cozzolino S, Fiori M. 2003. Phytotoxicity to and uptake of enrofloxacin in crop plants. Chemosphere, 52, 1233-1244.

[18]Nikolaou A, Meric S, Fatta D. 2007. Occurrence patterns of pharmaceuticals in water and wastewater environments. Analytical and Bioanalytical Chemistry, 387, 1225-1234.

[19]Park S, Choi K. 2008. Hazard assessment of commonly used agricultural antibiotics on aquatic ecosystems. Ecotoxicology, 17, 526-538.

[20]Sarmah A K, Meyer M T, Boxall A B A. 2006. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (Vas) in the environment. Chemosphere, 65, 725-759.

[21]Simon N S. 2005. Loosely bound oxytetracycline in riverine sediments from two tributaries of the Chesapeake Bay. Environmental Sciences & Technology, 39, 3480-3487.

[22]Ternes T, Hirsch R, Mueller J, Haberer K. 1998. Methods for the determination of neutral drugs as well as betablockers and ??2-sympathomimetics in aqueous matrices using GC/ MS and LC/MS/MS. Fresenius’ Journal of Analytical Chemistry, 362, 329-340.

[23]Thiele-Bruhn S. 2003. Pharmaceutical antibiotic compounds in soils - a review. Jounal of Plant Nutrition and Soil Science, 166, 145-167.

[24]Thaker P D. 2005. Pharmaceutical data elude researchers. Environmental Sciences & Technology, 139, 193A-194A.

[25]Xie X Y, Zhang Y Q, Li Z J, Liang Y C, Yao J H, Zhang S Q. 2009. Cultiva differences in toxic effects of oxytetracycline on wheat (Triticum aestivum L.). Asian Journal of Ecotoxicology, 4, 577-583. (in Chinese)

[26]Xu W H, Zhang G, Li X D, Zou S C, Hu Z H, Li J. 2007. Occurrence and elimination of antibiotics at four sewage treatment plants in the Pearl River Delta (PRD), South China. Water Research, 41, 4526-4534.

[27]Zhang H M, Zhang M K, Gu G P. 2008. Residues of tetracyclines in livestock and poultry manures and agricultral soils from north Zhejiang Province. Journal of Ecology Rural Environment, 24, 69-73. (in Chinese)

[28]Zhang S Q, Zhang F D, Liu X M, Wang Y J, Zhou S W, He X S. 2005. Determination and analysis on main harmful composition in excrement of scale livestock and poultry feedlots. Plant Nutrition and Fertilizing Science, 11, 822- 829. (in Chinese)

[29]Zuccato E, Calamari D, Natangelo M, Fanelli R. 2000. Presence of therapeutic drugs in the environment. Lancet, 335, 1789- 1790.

Negative Effects of Oxytetracycline on Wheat (Triticum aestivum L.) Growth, Root Activity, Photosynthesis, and Chlorophyll Contents

Negative Effects of Oxytetracycline on Wheat (Triticum aestivum L.) Growth, Root Activity, Photosynthesis, and Chlorophyll Contents

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