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1. 基于生理和转录组解析褪黑素提高杜鹃花热耐受的机制
XU Yan-xia, ZHANG Jing, WAN Zi-yun, HUANG Shan-xia, DI Hao-chen, HE Ying, JIN Song-heng
Journal of Integrative Agriculture    2023, 22 (8): 2397-2411.   DOI: 10.1016/j.jia.2023.07.005
摘要319)      PDF    收藏

杜鹃花是杜鹃花属的总称,是具有很高的观赏和经济价值的著名木本植物。热胁迫是影响杜鹃花生长的主要环境因子。褪黑素近年来被报道可以缓解非生物胁迫对植物的影响。然而,褪黑素在杜鹃花中的作用尚不清楚。本研究探讨了褪黑素对热胁迫下杜鹃花的影响及其潜在机制。叶绿素荧光结果表明,喷施200 µmol L-1褪黑素对杜鹃花抵抗热胁迫效果最佳。为了阐明褪黑素如何限制高温的不利影响,本研究分别在25、35和40°C处理下分析了杜鹃花叶片中褪黑素含量、光合参数、Rubisco酶活性和ATP含量。结果表明,与对照相比,外源喷施褪黑素提高了杜鹃花叶片中褪黑素含量、热应激下的电子传递速率、光系统II和I活性、Rubisco酶活性和ATP含量。转录组分析结果表明,许多热胁迫下的差异表达基因富集在光合作用途径,这些基因中的大部分均在热处理后下调表达,且在无褪黑素处理的植株中下调幅度大于外源喷施褪黑素处理的植株。本研究鉴定出RhPGR5ARhATPBRhLHCB3RhRbsA作为关键基因。综合以上结果,我们推测褪黑素通过调控包括RhRbsA在内的特定基因的表达,促进光合电子传递,提高卡尔文循环酶活性,增加ATP的产生,由此提高了热应激下的光合效率和CO2同化能力。因此,施用外源褪黑素可提高杜鹃花对热胁迫的耐受性。

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2. Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat
FANG Zheng-wu, HE Yi-qin, LIU Yi-ke, JIANG Wen-qiang, SONG Jing-han, WANG Shu-ping, MA Dong-fang, YIN Jun-liang
Journal of Integrative Agriculture    2020, 19 (5): 1170-1185.   DOI: 10.1016/S2095-3119(19)62776-0
摘要141)      PDF    收藏
Non-specific lipid transfer proteins (nsLTPs/LTPs) that can transport various phospholipids across the membrane in vitro are widespread in the plant kingdom, and they play important roles in many biological processes that are closely related to plant growth and development.  Recently, nsLTPs have been shown to respond to different forms of abiotic stresses.  Despite the vital roles of nsLTPs in many plants, little is known about the nsLTPs in wheat.  In this study, 330 nsLTP proteins were identified in wheat and they clustered into five types (1, 2, c, d, and g) by phylogenetic analysis with the nsLTPs from maize, Arabidopsis, and rice.  The wheat nsLTPs of type d included three subtypes (d1, d2, and d3) and type g included seven subtypes (g1–g7).  Genetic structure and motif pattern analyses showed that members of each type had similar structural composition.  Moreover, GPI-anchors were found to exist in non-g type members from wheat for the first time.  Chromosome mapping revealed that all five types were unevenly and unequally distributed on 21 chromosomes.  Furthermore, gene duplication events contributed to the proliferation of the nsLTP genes.  Large-scale data mining of RNA-seq data covering multiple growth stages and numerous stress treatments showed that the transcript levels of some of the nsLTP genes could be strongly induced by abiotic stresses, including drought and salinity, indicating their potential roles in mediating the responses of the wheat plants to these abiotic stress conditions.  These findings provide comprehensive insights into the nsLTP family members in wheat, and offer candidate nsLTP genes for further studies on their roles in stress resistance and potential for improving wheat breeding programs.
 
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3. Genome-wide detection of selective signatures in a Duroc pig population
DIAO Shu-qi, LUO Yuan-yu, MA Yun-long, DENG Xi, HE Ying-ting, GAO Ning, ZHANG Hao, LI Jia-qi, CHEN Zan-mou, ZHANG Zhe
Journal of Integrative Agriculture    2018, 17 (11): 2528-2535.   DOI: 10.1016/S2095-3119(18)61984-7
摘要429)      PDF    收藏
The Duroc pig has high adaptability and feeding efficiency, making it one of the most popular pig breeds worldwide.  Over long periods of natural and artificial selection, genetic footprints, i.e., selective signatures, were left in the genome.  In this study, a Duroc pig population (n=715) was genotyped with the Porcine SNP60K Bead Chip and the GeneSeek Genomic Profiler (GGP) Porcine Chip.  The relative extended haplotype homozygosity (REHH) method was used for selective signature detection in a subset of the population (n=368), selected to represent a balanced family structure.  In total, 154 significant core regions were detected as selective signatures (P<0.01), some of which overlap with previously reported quantitative trait loci associated with several economically important traits, including average daily gain and backfat thickness.  Genome annotation for these significant core regions revealed a variety of interesting candidate genes including GATA3, TAF3, ATP5C1, and FGF1.  These genes were functionally related to anterior/posterior pattern specification, phosphatidylinositol 3-kinase signaling, embryonic skeletal system morphogenesis, and oxidation-reduction processes.  This research provides knowledge for the study of selection mechanisms and breeding practices in Duroc and other pigs.
 
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4. Using a process-oriented methodology to precisely evaluate temperature suitability for potato growth in China using GIS
HE Ying-bin, ZHOU Yang-fan, CAI Wei-min, WANG Zhuo-zhuo, DUAN Ding-ding, LUO Shan-jun, CHEN Jing-zhu
Journal of Integrative Agriculture    2017, 16 (07): 1520-1529.   DOI: 10.1016/S2095-3119(16)61627-1
摘要813)      PDF    收藏
    A process-oriented methodology to conduct precise evaluation temporally and spatially on temperature suitability for potato growth was applied in China. Arable lands in China were gridded with 1 km×1 km geographic units, and potential potato phenology in each unit was automatically identified in terms of the potato planting initial temperature and effective accumulated temperature. A temperature thermal response coefficient model was used to compute a temperature suitability value for each day of potato phenology in each geographic unit. In addition, five temperature suitability ranking methods were applied to define suitable areas: (1) upper fourth quantile, (2) median, (3) expected value+1/4 standard deviation, (4) expected value+1/2 standard deviation, (5) expected value+1 standard deviation. A validation indicator was innovated to test the effectiveness of the five ranking methods. The results showed that from a strict degree point of view, the five methods sequence was as follows: 1=3>4>2>5, with a and c determined as the two best ranking methods. For methods 1 and 3, the suitable potato growing area was 1 of 57.76×104 km2. In addition, the suitable areas were spatially coincident with the main potato producing counties. The study output technically supports the proposal from China’s government that there is a large potential area to grow winter-ploughed potato in South China because the potential suitable area for growing potato is approximately 2×107 ha. In southeast Heilongjiang and east Jilin, where it is hilly and mountainous, there are still some potentially suitable areas for potato growing accounting for nearly 2.32×106 ha. The authors suggest to optimize the agricultural regionalization and layout in China and to adjust the cropping pattern structure.
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5. Linking a farmer crop selection model (FCS) with an agronomic model (EPIC) to simulate cropping pattern in Northeast China
HE Ying-bin, CAI Wei-min
Journal of Integrative Agriculture    2016, 15 (10): 2417-2425.   DOI: 10.1016/S2095-3119(15)61247-3
摘要1552)      PDF    收藏
  In this paper, authors established a farmer crop selection model (FCS) for the three provinces of Liaoning, Jilin and Heilongjiang of the Northeast China. With linking to the environmental policy integrated climate model (EPIC), the simulated results of FCS model for maize, rice and soybean were spatialized with 1 km×1 km grids to obtain cropping pattern. The reference map of spatial distribution for the three staple crops acquired by remote sensing imageries was applied to validate the simulated cropping pattern. The results showed that (1) the total simulation accuracy for the study area was 78.62%, which proved simulation method was applicable and feasible; (2) simulation accuracy for Jilin Province was the highest among the three provinces with a rate of 82.45% since its simple cropping system and not complex topography; (3) simulation accuracy for maize was the best among the three staple crops with a ratio of 81.14% because the study area is very suitable for maize growth. We hope this study could provide the reference for cropping pattern forecasting and decision-making.
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6. Current status and trends of wheat genetic transformation studies in China
HE Yi, WANG Qiong, ZENG Jian, SUN Tao, YANG Guang-xiao, HE Guang-yuan
Journal of Integrative Agriculture    2015, 14 (3): 438-452.   DOI: 10.1016/S2095-3119(14)60934-5
摘要2286)      PDF    收藏
More than 20 years have passed since the first report on successful genetic transformation of wheat. With the establishment and improvement of transformation platform, great progresses have been made on wheat genetic transformation both on its fundamental and applied studies in China, especially driven by the National Major Project for Transgenic Organism Breeding, China, initiated in 2008. In this review, wheat genetic transformation platform improvement and transgenic research progresses including new techniques applied and functional studies of wheat quality, yield and stress tolerant related genes and biosafety assessment are summarized. The existing problems and the trends in wheat transformation with traditional methods combined with genomic studies and genome editing technology are also discussed.
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