Muscle Biological Characteristics of Differentially Expressed Genes in Wujin and Landrace Pigs

doi:10.1016/S2095-3119(13)60605-X

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (10): 2236-2242.DOI: 10.1016/S2095-3119(13)60605-X

Muscle Biological Characteristics of Differentially Expressed Genes in Wujin and Landrace Pigs

XU Hong, HUANG Ying, LI Wei-zhen, YANG Ming-hua, GE Chang-rong, ZHANG Xi, LI　Liu-an, GAO Shi-zheng , ZHAO Su-mei

1、School of Public Finance and Economics, Yunnan University of Finance and Economics, Kunming 650221, P.R.China
2、Yunnan Key Laboratory of Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming 650201, P.R.China
3、Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, P.R.China
4、Animal Husbandry Post-Doctoral Station, Nanjing Agricultural University, Nanjing 210095, P.R.China
5、Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, P.R.China

收稿日期:2013-04-15 出版日期:2014-10-01 发布日期:2014-10-10
通讯作者: ZHAO Su-mei, E-mail: zhaosm2009@126.com
作者简介:XU Hong, E-mail: xuhong68@126.com
基金资助:
This work was sponsored by the Yunnan Natural Science Foundation, China (2009CD056), the National Natural Science foundation of China (30660132, 31060331 and 31260592), the Special Program for Key Basic Research of the Ministry of Science and Technology, China (2007CB116201) and the National Key Program of Transgenic Project of China (2009ZX08009-140B).

Muscle Biological Characteristics of Differentially Expressed Genes in Wujin and Landrace Pigs

XU Hong, HUANG Ying, LI Wei-zhen, YANG Ming-hua, GE Chang-rong, ZHANG Xi, LI　Liu-an, GAO Shi-zheng , ZHAO Su-mei

1、School of Public Finance and Economics, Yunnan University of Finance and Economics, Kunming 650221, P.R.China
2、Yunnan Key Laboratory of Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming 650201, P.R.China
3、Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, P.R.China
4、Animal Husbandry Post-Doctoral Station, Nanjing Agricultural University, Nanjing 210095, P.R.China
5、Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, P.R.China

Received:2013-04-15 Online:2014-10-01 Published:2014-10-10
Contact: ZHAO Su-mei, E-mail: zhaosm2009@126.com
About author:XU Hong, E-mail: xuhong68@126.com
Supported by:
This work was sponsored by the Yunnan Natural Science Foundation, China (2009CD056), the National Natural Science foundation of China (30660132, 31060331 and 31260592), the Special Program for Key Basic Research of the Ministry of Science and Technology, China (2007CB116201) and the National Key Program of Transgenic Project of China (2009ZX08009-140B).

摘要/Abstract

摘要： The biological chemistry would be responsible for the meat quality. This study tried to investigate the transcript expression profile and explain the characteristics of differentially expressed genes between the Wujin and Landrace pigs. The results showed that 526 differentially expressed genes were found by comparing the transcript expression profile of muscle tissue between Wujin and Landrace pigs. Among them, 335 genes showed up-regulations and 191 genes showed down-regulations in Wujin pigs compared with the Landrace pigs. Gene ontology (GO) analysis indicated that the differentially expressed genes were clustered into three groups involving in protein synthesis, energy metabolism and immune response. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis found that these differentially expressed genes participated in protein synthesis metabolism, energy metabolism and immune response pathway. The Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis of protein function and protein domains function also confirmed that differentially expressed genes belonged to protein synthesis, energy metabolism and immune response. Genes related protein synthesis metabolism pathway in Landrace was higher than in Wujin pigs. However, differentially expressed genes related energy metabolism and immune response was up-regulated in Wujin pigs compared with Landrace pigs. Quantitative real-time RT-PCR on selected genes was used to confirm the results from the microarray. These suggested that the genes related to protein synthesis, energy metabolism and immune response would contribute to the growth performance, meat quality as well as anti-disease capacity.

关键词: pigs , muscle tissue , microarray , bioinformatics analysis , differentially expressed genes

Abstract: The biological chemistry would be responsible for the meat quality. This study tried to investigate the transcript expression profile and explain the characteristics of differentially expressed genes between the Wujin and Landrace pigs. The results showed that 526 differentially expressed genes were found by comparing the transcript expression profile of muscle tissue between Wujin and Landrace pigs. Among them, 335 genes showed up-regulations and 191 genes showed down-regulations in Wujin pigs compared with the Landrace pigs. Gene ontology (GO) analysis indicated that the differentially expressed genes were clustered into three groups involving in protein synthesis, energy metabolism and immune response. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis found that these differentially expressed genes participated in protein synthesis metabolism, energy metabolism and immune response pathway. The Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis of protein function and protein domains function also confirmed that differentially expressed genes belonged to protein synthesis, energy metabolism and immune response. Genes related protein synthesis metabolism pathway in Landrace was higher than in Wujin pigs. However, differentially expressed genes related energy metabolism and immune response was up-regulated in Wujin pigs compared with Landrace pigs. Quantitative real-time RT-PCR on selected genes was used to confirm the results from the microarray. These suggested that the genes related to protein synthesis, energy metabolism and immune response would contribute to the growth performance, meat quality as well as anti-disease capacity.

Key words: pigs , muscle tissue , microarray , bioinformatics analysis , differentially expressed genes

XU Hong, HUANG Ying, LI Wei-zhen, YANG Ming-hua, GE Chang-rong, ZHANG Xi, LI　Liu-an, GAO Shi-zheng , ZHAO Su-mei. Muscle Biological Characteristics of Differentially Expressed Genes in Wujin and Landrace Pigs[J]. Journal of Integrative Agriculture, 2014, 13(10): 2236-2242.

XU Hong, HUANG Ying, LI Wei-zhen, YANG Ming-hua, GE Chang-rong, ZHANG Xi, LI　Liu-an , GAO Shi-zheng , ZHAO Su-mei. Muscle Biological Characteristics of Differentially Expressed Genes in Wujin and Landrace Pigs[J]. Journal of Integrative Agriculture, 2014, 13(10): 2236-2242.

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Muscle Biological Characteristics of Differentially Expressed Genes in Wujin and Landrace Pigs

Muscle Biological Characteristics of Differentially Expressed Genes in Wujin and Landrace Pigs

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