Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

doi:10.1016/S2095-3119(13)60414-1

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (2): 378-386.DOI: 10.1016/S2095-3119(13)60414-1

Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

ZHAO Su-mei, LIU Yong-gang, PAN Hong-bing, ZHANG Xi, GE Chang-rong, JIA Jun-jing , GAO Shi-zheng

Yunnan Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming 650201, P.R.China

收稿日期:2012-10-11 出版日期:2014-02-01 发布日期:2014-02-06
通讯作者: ZHAO Su-mei, Tel: +86-871-65227796, Fax: +86-871-65227284, E-mail: zhaosm2009@126.com
作者简介:ZHAO Su-mei, Tel: +86-871-65227796, Fax: +86-871-65227284, E-mail: zhaosm2009@126.com
基金资助:
This work was supported by the National Basic Research Program of China (2007CB116201).

Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

ZHAO Su-mei, LIU Yong-gang, PAN Hong-bing, ZHANG Xi, GE Chang-rong, JIA Jun-jing , GAO Shi-zheng

Yunnan Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming 650201, P.R.China

Received:2012-10-11 Online:2014-02-01 Published:2014-02-06
Contact: ZHAO Su-mei, Tel: +86-871-65227796, Fax: +86-871-65227284, E-mail: zhaosm2009@126.com
About author:ZHAO Su-mei, Tel: +86-871-65227796, Fax: +86-871-65227284, E-mail: zhaosm2009@126.com
Supported by:
This work was supported by the National Basic Research Program of China (2007CB116201).

摘要/Abstract

摘要： Porcine skeletal muscle genes play a major role in determining muscle growth and meat quality. Construction of a full-length cDNA library is an effective way to understand the expression of functional genes in muscle tissues. In addition, novel genes for further research could be identified in the library. In this study, we constructed a full-length cDNA library from porcine muscle tissue. The estimated average size of the cDNA inserts was 1 076 bp, and the cDNA fullness ratio was 86.2%. A total of 1 058 unique sequences with 342 contigs (32.3%) and 716 singleton (67.7%) expressed sequence tags (EST) were obtained by clustering and assembling. Meanwhile, 826 (78.1%) ESTs were categorized as known genes, and 232 (21.9%) ESTs were categorized as unknown genes. 65 novel porcine genes that exhibit no identity in the TIGR gene index of Sus scrofa and 124 full-length sequences with unknown functions were deposited in the dbEST division of GenBank (accession numbers: EU650784-EU650788, GE843306, GH228978-GH229100). The abundantly expressed genes in porcine muscle tissue were related to muscle fiber development, energy metabolism and protein synthesis. Gene ontology analysis showed that sequences expressed in porcine muscle tissue contained a high percentage of binding activity, catalytic activity, structural molecule activity and motor activity, which involved mainly in metabolic, cellular and developmental process, distributed mainly in intracellular region. The sequence data generated in this study would provide valuable information for identifying porcine genes expressed in muscle tissue and help to advance the study on the structure and function of genes in pigs.

关键词: muscle tissue , full-length cDNA library , expressed sequence tag , pig

Abstract: Porcine skeletal muscle genes play a major role in determining muscle growth and meat quality. Construction of a full-length cDNA library is an effective way to understand the expression of functional genes in muscle tissues. In addition, novel genes for further research could be identified in the library. In this study, we constructed a full-length cDNA library from porcine muscle tissue. The estimated average size of the cDNA inserts was 1 076 bp, and the cDNA fullness ratio was 86.2%. A total of 1 058 unique sequences with 342 contigs (32.3%) and 716 singleton (67.7%) expressed sequence tags (EST) were obtained by clustering and assembling. Meanwhile, 826 (78.1%) ESTs were categorized as known genes, and 232 (21.9%) ESTs were categorized as unknown genes. 65 novel porcine genes that exhibit no identity in the TIGR gene index of Sus scrofa and 124 full-length sequences with unknown functions were deposited in the dbEST division of GenBank (accession numbers: EU650784-EU650788, GE843306, GH228978-GH229100). The abundantly expressed genes in porcine muscle tissue were related to muscle fiber development, energy metabolism and protein synthesis. Gene ontology analysis showed that sequences expressed in porcine muscle tissue contained a high percentage of binding activity, catalytic activity, structural molecule activity and motor activity, which involved mainly in metabolic, cellular and developmental process, distributed mainly in intracellular region. The sequence data generated in this study would provide valuable information for identifying porcine genes expressed in muscle tissue and help to advance the study on the structure and function of genes in pigs.

Key words: muscle tissue , full-length cDNA library , expressed sequence tag , pig

ZHAO Su-mei, LIU Yong-gang, PAN Hong-bing, ZHANG Xi, GE Chang-rong, JIA Jun-jing , GAO Shi-zheng. Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig[J]. Journal of Integrative Agriculture, 2014, 13(2): 378-386.

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Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

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