Genetic Analysis and Preliminary Mapping of a Highly Male-Sterile Gene in Foxtail Millet (Setaria italica L. Beauv.) Using SSR Markers

doi:10.1016/S2095-3119(13)60392-5

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (12): 2143-2148.DOI: 10.1016/S2095-3119(13)60392-5

Genetic Analysis and Preliminary Mapping of a Highly Male-Sterile Gene in Foxtail Millet (Setaria italica L. Beauv.) Using SSR Markers

WANG Jun, WANG Zhi-lan, YANG Hui-qing, YUAN Feng, GUO Er-hu, TIAN Gang, AN Yuan-

1.College of Agriculture, Shanxi Agricultural University, Taigu 030800, P.R.China
2.Millet Research Institute, Shanxi Academy of Agricultural Sciences, Changzhi 046011, P.R.China
3.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2013-03-05 出版日期:2013-12-01 发布日期:2013-12-17
通讯作者: GUO Ping-yi, Mobile: 13903446155, E-mail: pyguo@sxau.edu.cn
作者简介:WANG Jun, Mobile: 13333550810, E-mail: 128wan@163.com
基金资助:
This research was supported by the Postdoctoral Management Committee, China (92948), the Natural Science Foundation of Shanxi Province, China (2012011032-1) and the Chinese Agricultural Research System (CARS-07).

Genetic Analysis and Preliminary Mapping of a Highly Male-Sterile Gene in Foxtail Millet (Setaria italica L. Beauv.) Using SSR Markers

WANG Jun, WANG Zhi-lan, YANG Hui-qing, YUAN Feng, GUO Er-hu, TIAN Gang, AN Yuan-

1.College of Agriculture, Shanxi Agricultural University, Taigu 030800, P.R.China
2.Millet Research Institute, Shanxi Academy of Agricultural Sciences, Changzhi 046011, P.R.China
3.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2013-03-05 Online:2013-12-01 Published:2013-12-17
Contact: GUO Ping-yi, Mobile: 13903446155, E-mail: pyguo@sxau.edu.cn
About author:WANG Jun, Mobile: 13333550810, E-mail: 128wan@163.com
Supported by:
This research was supported by the Postdoctoral Management Committee, China (92948), the Natural Science Foundation of Shanxi Province, China (2012011032-1) and the Chinese Agricultural Research System (CARS-07).

摘要/Abstract

摘要： Breeding of male-sterile lines has become the mainstream for the heterosis utilization in foxtail millet, but the genetic basis of most male-sterile lines used for the hybrid is still an area to be elucidated. In this study, a highly male-sterile line Gao146A was investigated. Genetic analysis indicated that the highly male-sterile phenotype was controlled by a single recessive gene a single recessive gene. Using F2 population derived from cross Gao146A/K103, one gene controlling the highly male- sterility, tentatively named as ms1, which linked to SSR marker b234 with genetic distance of 16.7 cM, was mapped on the chromosome VI. These results not only laid the foundation for fine mapping of this highly male-sterile gene, but also helped to accelerate the improvement of highly male-sterile lines by using molecular marker assisted breeding method.

关键词: foxtail millet (Setaria italic L. Beauv.) , highly male-sterility , gene mapping , SSR marker

Abstract: Breeding of male-sterile lines has become the mainstream for the heterosis utilization in foxtail millet, but the genetic basis of most male-sterile lines used for the hybrid is still an area to be elucidated. In this study, a highly male-sterile line Gao146A was investigated. Genetic analysis indicated that the highly male-sterile phenotype was controlled by a single recessive gene a single recessive gene. Using F2 population derived from cross Gao146A/K103, one gene controlling the highly male- sterility, tentatively named as ms1, which linked to SSR marker b234 with genetic distance of 16.7 cM, was mapped on the chromosome VI. These results not only laid the foundation for fine mapping of this highly male-sterile gene, but also helped to accelerate the improvement of highly male-sterile lines by using molecular marker assisted breeding method.

Key words: foxtail millet (Setaria italic L. Beauv.) , highly male-sterility , gene mapping , SSR marker

WANG Jun, WANG Zhi-lan, YANG Hui-qing, YUAN Feng, GUO Er-hu, TIAN Gang, AN Yuan-. Genetic Analysis and Preliminary Mapping of a Highly Male-Sterile Gene in Foxtail Millet (Setaria italica L. Beauv.) Using SSR Markers[J]. Journal of Integrative Agriculture, 2013, 12(12): 2143-2148.

WANG Jun, WANG Zhi-lan, YANG Hui-qing, YUAN Feng, GUO Er-hu, TIAN Gang, AN Yuan. Genetic Analysis and Preliminary Mapping of a Highly Male-Sterile Gene in Foxtail Millet (Setaria italica L. Beauv.) Using SSR Markers[J]. Journal of Integrative Agriculture, 2013, 12(12): 2143-2148.

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Genetic Analysis and Preliminary Mapping of a Highly Male-Sterile Gene in Foxtail Millet (Setaria italica L. Beauv.) Using SSR Markers

Genetic Analysis and Preliminary Mapping of a Highly Male-Sterile Gene in Foxtail Millet (Setaria italica L. Beauv.) Using SSR Markers

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