Structure, Binding Characteristics, and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm, Helicoverpa armigera (Hübner)

doi:10.1016/S1671-2927(00)8561

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (3): 430-438.DOI: 10.1016/S1671-2927(00)8561

Structure, Binding Characteristics, and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm, Helicoverpa armigera (Hübner)

href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((ZHANG Tian-tao[Author]) AND 1[Journal]) AND year[Order])" target="_blank">ZHANG Tian-tao, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((WANG Wei-xuan[Author]) AND 1[Journal]) AND year[Order])" target="_blank">WANG Wei-xuan, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((ZHANG Zi-ding[Author]) AND 1[Journal]) AND year[Order])" target="_blank">ZHANG Zi-ding, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((ZHANG Yongjun[Author]) AND 1[Journal]) AND year[Order])" target="_blank">ZHANG Yongjun, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((GUO Yu-yuan[Author]) AND 1[Journal]) AND year[Order])" target="_blank">GUO Yu-yuan

1.Northwest Agriculture and Forestry University, Yangling 712100, P.R.China
2.State Key Laboratory of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3.State Key Laboratory of Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, P.R.China

收稿日期:2011-03-29 出版日期:2012-03-01 发布日期:2012-03-11
通讯作者: Correspondence ZHANG Yong-jun, Tel: +86-10-62815929, Fax: +86-10-62894786, E-mail: yjzhang@ippcaas.cn; GUO Yu-yuan, Tel/Fax: +86-10-62894786,E-mail: yuyuanguo@hotmail.com
基金资助:
This work was supported by the National Basic Research Program of China (2012CB114104), the National Natural Science Foundation of China (30871640, 31071694), the National High-Tech R&D Program of China (2008AA02Z307), and the International Cooperation and Exchange Foundation of NSFC-RS of China (31111130203).

Structure, Binding Characteristics, and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm, Helicoverpa armigera (Hübner)

ZHANG Tian-tao, WANG Wei-xuan, ZHANG Zi-ding, ZHANG Yongjun, GUO Yu-yuan

1.Northwest Agriculture and Forestry University, Yangling 712100, P.R.China
2.State Key Laboratory of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3.State Key Laboratory of Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, P.R.China

Received:2011-03-29 Online:2012-03-01 Published:2012-03-11
Contact: Correspondence ZHANG Yong-jun, Tel: +86-10-62815929, Fax: +86-10-62894786, E-mail: yjzhang@ippcaas.cn; GUO Yu-yuan, Tel/Fax: +86-10-62894786,E-mail: yuyuanguo@hotmail.com
Supported by:
This work was supported by the National Basic Research Program of China (2012CB114104), the National Natural Science Foundation of China (30871640, 31071694), the National High-Tech R&D Program of China (2008AA02Z307), and the International Cooperation and Exchange Foundation of NSFC-RS of China (31111130203).

摘要/Abstract

摘要： The full-length sequence of the odorant binding protein 5 gene, HarmOBP5, was obtained from an antennae cDNA library of cotton bollworm, Helicoverpa armigera (Hübner). The cDNA contains a 444 bp open reading frame, encoding a protein with 147 amino acids, namely HarmOBP5. HarmOBP5 was expressed in Escherichia coli and the recombinant protein was purified by affinity chromatography. SDS-PAGE and Western blot analysis demonstrated that the purified protein can be used for further investigation of its binding characteristics. Competitive binding assays with 113 odorant chemicals indicated that HarmOBP5 has strong affinity to some special plant volatiles, including (E)-β-farnesene, ethyl butyrate, ethyl heptanoate, and acetic acid 2-methylbutyl ester. Based on three-dimensional (3D) model of AaegOBP1 from Aedes aegypti, a 3D model of HarmOBP5 was predicted. The model revealed that some key binding residues in HarmOBP5 may play important roles in odorant perception of H. armigera. This study provides clues for better understanding physiological functions of OBPs in H. armigera and other insects.

关键词: odorant binding protein, fluorescence binding assay, 3D model, Helicoverpa armigera (Hü, bner)

Abstract: The full-length sequence of the odorant binding protein 5 gene, HarmOBP5, was obtained from an antennae cDNA library of cotton bollworm, Helicoverpa armigera (Hübner). The cDNA contains a 444 bp open reading frame, encoding a protein with 147 amino acids, namely HarmOBP5. HarmOBP5 was expressed in Escherichia coli and the recombinant protein was purified by affinity chromatography. SDS-PAGE and Western blot analysis demonstrated that the purified protein can be used for further investigation of its binding characteristics. Competitive binding assays with 113 odorant chemicals indicated that HarmOBP5 has strong affinity to some special plant volatiles, including (E)-β-farnesene, ethyl butyrate, ethyl heptanoate, and acetic acid 2-methylbutyl ester. Based on three-dimensional (3D) model of AaegOBP1 from Aedes aegypti, a 3D model of HarmOBP5 was predicted. The model revealed that some key binding residues in HarmOBP5 may play important roles in odorant perception of H. armigera. This study provides clues for better understanding physiological functions of OBPs in H. armigera and other insects.

Key words: odorant binding protein, fluorescence binding assay, 3D model, Helicoverpa armigera (Hübner)

ZHANG Tian-tao, WANG Wei-xuan, ZHANG Zi-ding, ZHANG Yongjun, GUO Yu-yuan. Structure, Binding Characteristics, and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm, Helicoverpa armigera (Hübner)[J]. Journal of Integrative Agriculture, 2012, 12(3): 430-438.

ZHANG Tian-tao, WANG Wei-xuan, ZHANG Zi-ding, ZHANG Yongjun, GUO Yu-yuan, . Structure, Binding Characteristics, and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm, Helicoverpa armigera (Hübner)[J]. Journal of Integrative Agriculture, 2012, 12(3): 430-438.

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Structure, Binding Characteristics, and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm, Helicoverpa armigera (Hübner)

Structure, Binding Characteristics, and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm, Helicoverpa armigera (Hübner)

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