Comparative Analysis of Cry1Ac Toxin Oligomerization and Pore Formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae

doi:10.1016/S1671-2927(00)8719

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (11): 1845-1851.DOI: 10.1016/S1671-2927(00)8719

Comparative Analysis of Cry1Ac Toxin Oligomerization and Pore Formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae

LI Yi-ping, WUJun-xiang, LIU Chen-xi, YUAN Xiang-qun, WU Kong-ming, LIANG Ge-mei, GUO Yu-yuan

1.Key Laboratory of Applied Entomology; Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education/College of Plant Protection, Northwest Agriculture and Forestry University, Yangling 712100, P.R.China
2.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

收稿日期:2011-10-17 出版日期:2012-11-01 发布日期:2012-11-24
通讯作者: Correspondence GUO Yu-yuan, Tel/Fax: +86-10-62894786, E-mail: yuyuanguo@hotmail.com;LIANG Ge-mei, Tel: +86-10-62815929, E-mail: gmliang@ippcaas.cn
作者简介:LI Yi-ping, Mobile: 13759967722, E-mail: liyiping@nwsuaf.edu.cn
基金资助:
This research was supported by the National Basic Research Program of China (31071693).

Comparative Analysis of Cry1Ac Toxin Oligomerization and Pore Formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae

LI Yi-ping, WUJun-xiang, LIU Chen-xi, YUAN Xiang-qun, WU Kong-ming, LIANG Ge-mei, GUO Yu-yuan

1.Key Laboratory of Applied Entomology; Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education/College of Plant Protection, Northwest Agriculture and Forestry University, Yangling 712100, P.R.China
2.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

Received:2011-10-17 Online:2012-11-01 Published:2012-11-24
Contact: Correspondence GUO Yu-yuan, Tel/Fax: +86-10-62894786, E-mail: yuyuanguo@hotmail.com;LIANG Ge-mei, Tel: +86-10-62815929, E-mail: gmliang@ippcaas.cn
About author:LI Yi-ping, Mobile: 13759967722, E-mail: liyiping@nwsuaf.edu.cn
Supported by:
This research was supported by the National Basic Research Program of China (31071693).

摘要/Abstract

摘要： With the long-term use of Bacillus thuringiensis (Bt) insecticide and expansion of Cry1A-expressing transgenic plants, some insect pests have developed resistance to Bt in open fields, greenhouses, and in the laboratory. Bt resistance is complex and there appear to be different ways for resistance development. Understanding the Bt resistance mechanisms is critical to prolong its usefulness. In this article, Bt receptors, the cadherin and aminopeptidase N (APN), in brush border membrane vesicles (BBMV) of Helicoverpa armigera were examined in both Cry1Ac-susceptible (96S) and Cry1Acresistant (LF120) strains, to compare Cry1Ac toxin oligomerization and pore formation in these two strains. Cry1Ac toxin oligomerization and pore formation in these two strains were compared. Results showed that cadherin and aminopeptidase N proteins could express normally in both susceptible and resistant H. armigera strains. The ability to form Cry1Ac oligomers and ion channels on BBMVs was also not significantly different between these two strains.

关键词: Helicoverpa armigera , Bt resistance , oligomerization , pore formation

Abstract: With the long-term use of Bacillus thuringiensis (Bt) insecticide and expansion of Cry1A-expressing transgenic plants, some insect pests have developed resistance to Bt in open fields, greenhouses, and in the laboratory. Bt resistance is complex and there appear to be different ways for resistance development. Understanding the Bt resistance mechanisms is critical to prolong its usefulness. In this article, Bt receptors, the cadherin and aminopeptidase N (APN), in brush border membrane vesicles (BBMV) of Helicoverpa armigera were examined in both Cry1Ac-susceptible (96S) and Cry1Acresistant (LF120) strains, to compare Cry1Ac toxin oligomerization and pore formation in these two strains. Cry1Ac toxin oligomerization and pore formation in these two strains were compared. Results showed that cadherin and aminopeptidase N proteins could express normally in both susceptible and resistant H. armigera strains. The ability to form Cry1Ac oligomers and ion channels on BBMVs was also not significantly different between these two strains.

Key words: Helicoverpa armigera , Bt resistance , oligomerization , pore formation

LI Yi-ping, WUJun-xiang, LIU Chen-xi, YUAN Xiang-qun, WU Kong-ming, LIANG Ge-mei, GUO Yu-yuan. Comparative Analysis of Cry1Ac Toxin Oligomerization and Pore Formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae[J]. Journal of Integrative Agriculture, 2012, 12(11): 1845-1851.

LI Yi-ping, WUJun-xiang , LIU Chen-xi, YUAN Xiang-qun, WU Kong-ming, LIANG Ge-mei, GUO Yu-yuan. Comparative Analysis of Cry1Ac Toxin Oligomerization and Pore Formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae[J]. Journal of Integrative Agriculture, 2012, 12(11): 1845-1851.

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Comparative Analysis of Cry1Ac Toxin Oligomerization and Pore Formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae

Comparative Analysis of Cry1Ac Toxin Oligomerization and Pore Formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae

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