Characterization of Ethylene Receptors and Their Interactions with GmTPRA Novel Tetratricopeptide Repeat Protein (TPR) in Soybean (Glycine max L.)

doi:10.1016/S2095-3119(13)60274-9

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (4): 571-581.DOI: 10.1016/S2095-3119(13)60274-9

Characterization of Ethylene Receptors and Their Interactions with GmTPRA Novel Tetratricopeptide Repeat Protein (TPR) in Soybean (Glycine max L.)

NIU Yan-yan, CHEN Ming, XU Zhao-shi, LI Lian-cheng, CHEN Xue-ping , MA You-zhi

1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, P.R.China
2.The National Key Facility for Crop Genetic Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2012-05-11 出版日期:2013-04-01 发布日期:2013-04-07
基金资助:
The work was funded in part by the National Key Project for Research on Transgenic Biology (2011ZX08002-002 and 2011ZX08002-005) and the National High-Tech R&D Program of China (2012AA10A309).

Characterization of Ethylene Receptors and Their Interactions with GmTPRA Novel Tetratricopeptide Repeat Protein (TPR) in Soybean (Glycine max L.)

NIU Yan-yan, CHEN Ming, XU Zhao-shi, LI Lian-cheng, CHEN Xue-ping , MA You-zhi

1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, P.R.China
2.The National Key Facility for Crop Genetic Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2012-05-11 Online:2013-04-01 Published:2013-04-07
Contact: Correspondence CHEN Ming, Tel/Fax: +86-10-82108789, E-mail: chenming02@caas.cn; CHEN Xue-ping, Mobile: 13866573456, Fax: +86-10-82108789, E-mail: chenxp08@ustc.edu.cn
Supported by:
The work was funded in part by the National Key Project for Research on Transgenic Biology (2011ZX08002-002 and 2011ZX08002-005) and the National High-Tech R&D Program of China (2012AA10A309).

摘要/Abstract

摘要： Ethylene receptors play important roles not only in regulation of growth and development but also in response to environmental stimuli of plants. However, there are few reports on ethylene receptors in soybean. In this article, putative ethylene receptors of soybean were searched from soybean genomic database (http://www.phytozome.net/search.php) and analyzed. The ethylene receptor gene family in soybean comprising eight members, designated as GmERS1-1, GmERS1-2, GmETR1-1, GmETR1-2, GmETR2-1, GmETR2-2, GmEIN4-1, and GmEIN4-2 corresponding with their homologous genes in Arabidopsis, were isolated and analyzed. Phylogenetic analysis indicated that the eight soybean ethylene receptors (SERs) were in two subfamilies and further divided into four groups, viz., groups I (GmERS1-1 and GmERS1-2), II (GmETR1-1 and GmETR1-2), VI (GmETR2-1 and GmETR2-2), and VII (GmEIN4-1 and GmEIN4-2). Protein structure of the members in groups I and II from subfamily I were more conserved than the members in other two groups from subfamily II. Expression patterns of the SERs were compared with the homologous genes in Arabidopsis. The results demonstrated that expression patterns of the SERs differed from Arabidopsis members in the same group, suggesting that SERs are involved in different signal pathways compared to ethylene receptors in Arabidopsis. Promoter analysis showed that the sequences of the members in each group were different from each other, and some specific binding elements of transcription factors detected in promoter sequences might explain the differences between the members in the same group. A novel soybean TPR protein (tetratricopeptide repeat protein), GmTPR, was identified to interact with GmETR1-1, apparently an important ethylene receptor in ethylene signaling pathway in soybean. This suggested that GmTPR might be a novel downstream component of the ethylene signaling pathway.

关键词: soybean , Arabidopsis , ethylene receptor , TPR protein

Abstract: Ethylene receptors play important roles not only in regulation of growth and development but also in response to environmental stimuli of plants. However, there are few reports on ethylene receptors in soybean. In this article, putative ethylene receptors of soybean were searched from soybean genomic database (http://www.phytozome.net/search.php) and analyzed. The ethylene receptor gene family in soybean comprising eight members, designated as GmERS1-1, GmERS1-2, GmETR1-1, GmETR1-2, GmETR2-1, GmETR2-2, GmEIN4-1, and GmEIN4-2 corresponding with their homologous genes in Arabidopsis, were isolated and analyzed. Phylogenetic analysis indicated that the eight soybean ethylene receptors (SERs) were in two subfamilies and further divided into four groups, viz., groups I (GmERS1-1 and GmERS1-2), II (GmETR1-1 and GmETR1-2), VI (GmETR2-1 and GmETR2-2), and VII (GmEIN4-1 and GmEIN4-2). Protein structure of the members in groups I and II from subfamily I were more conserved than the members in other two groups from subfamily II. Expression patterns of the SERs were compared with the homologous genes in Arabidopsis. The results demonstrated that expression patterns of the SERs differed from Arabidopsis members in the same group, suggesting that SERs are involved in different signal pathways compared to ethylene receptors in Arabidopsis. Promoter analysis showed that the sequences of the members in each group were different from each other, and some specific binding elements of transcription factors detected in promoter sequences might explain the differences between the members in the same group. A novel soybean TPR protein (tetratricopeptide repeat protein), GmTPR, was identified to interact with GmETR1-1, apparently an important ethylene receptor in ethylene signaling pathway in soybean. This suggested that GmTPR might be a novel downstream component of the ethylene signaling pathway.

Key words: soybean , Arabidopsis , ethylene receptor , TPR protein

NIU Yan-yan, CHEN Ming, XU Zhao-shi, LI Lian-cheng, CHEN Xue-ping , MA You-zhi. Characterization of Ethylene Receptors and Their Interactions with GmTPRA Novel Tetratricopeptide Repeat Protein (TPR) in Soybean (Glycine max L.)[J]. Journal of Integrative Agriculture, 2013, 12(4): 571-581.

参考文献

[1]Bisson M A, Groth G. 2010. New insight in ethylenesignaling: autokinase activity of ETR1 modulates theinteraction of receptors and EIN2. Molecular Plant, 3,882-889

[2]Bisson M A, Groth G. 2011. New paradigm in ethylenesignaling: EIN2, the central regulator of the signalingpathway, interacts directly with the upstream receptors.Plant Signal Behavior, 6, 164-166

[3]Bradford K J, Trewavas A J. 1994. Sensitivity thresholdsand variable time scales in plant hormone action. PlantPhysiology, 105, 1029-1036

[4]Chang C, Shockey J A. 1999. The ethylene-responsepathway: signal perception to gene regulation. CurrentOpinion in Plant Biology, 2, 352-358

[5]Chen J F, Gallie D R. 2010. Analysis of the functionalconservation of ethylene receptors between maize andArabidopsis. Plant Molecular Biology, 74, 405-421

[6]Chen T, Liu J, Lei G, Liu Y F, Li Z G, Tao J J, Hao Y J, Cao RY, Lin Q, Zhang W K, et al. 2009. Effects of tobaccoethylene receptor mutations on receptor kinase activity,plant growth and stress responses. Plant and CellPhysiology, 50, 1636-1650

[7]Chen Y F, Randlett M D, Findell J L, Schaller G E. 2002.Localization of the ethylene receptor ETR1 to theendoplasmic reticulum of Arabidopsis. Journal ofBiological Chemistry, 277, 19861-19866

[8]Chow B, McCourt P. 2006. Plant hormone receptors:perception is everything. Genes Development, 20, 1998-2008

[9]Díaz J. 2011. Information flow in plant signaling pathways.Plant Signaling & Behavior, 6, 339-343

[10]Desikan R, Hancock J T, Bright J, Harrison J, Weir I, HooleyR, Neill S J. 2005. A role for ETR1 in hydrogen peroxidesignaling in stomatal guard cells. Plant Physiology,137, 831-834

[11]Dong C H, Jang M, Scharein B, Malach A, Rivarola M,Liesch J, Groth G, Hwang I, Chang C. 2010. Molecularassociation of the Arabidopsis ETR1 ethylene receptorand a regulator of ethylene signaling, RTE1. Journal ofBiological Chemistry, 285, 40706-40713

[12]Dong J, Wang X M, Wang K, Wang Z, Gao H W. 2012.Isolation and characterization of a gene encoding anethylene responsive factor protein from Ceratoidesarborescens. Molecular Biology Reports, 39, 1349-1357

[13]Gao Z, Schaller G E. 2009. The role of receptor interactionsin regulating ethylene signal transduction. Plant SignalBehavior, 4, 1152-1153

[14]Gregory L B, Michael L. 1999 The tetratricopeptide repeat:a structural motif mediating protein-protein interactions.BioEssays, 21, 932-939

[15]Guzman P, Ecker J R. 1990. Exploiting the triple response ofArabidopsis to identify ethylene-related mutants. ThePlant Cell, 2, 513-523

[16]Haider A J, Briggs D, Self T J, Chilvers H L, Holliday N D,Kerr I D. 2011. Dimerization of ABCG2 analysed bybimolecular fluorescence complementation. PLoS One,6, e25818.

[17]Hall A E, Bleecker A B. 2003. Analysis of combinatorialloss-of-function mutants in the Arabidopsis ethylenereceptors reveals that the ers1 etr1 double mutant hassevere developmental defects that are EIN2 dependent.The Plant Cell, 15, 2032-2041

[18]He X J, Zhang Z G, Yan D Q, Zhang J S, Chen S Y. 2004. Asalt-responsive receptor-like kinase gene regulated bythe ethylene signaling pathway encodes a plasmamembrane serine/threonine kinase. Theoretical and Applied Genetics, 109, 377-383

[19]Hua J, Meyerowitz E M. 1998. Ethylene responses arenegatively regulated by a receptor gene family inArabidopsis thaliana. Cell, 94, 261-271

[20]Huang Y, Li H, Hutchison C E, Laskey J, Kieber J J. 2003.Biochemical and functional analysis of CTR1, a proteinkinase that negatively regulates ethylene signaling inArabidopsis. The Plant Journal, 33, 221-233

[21]Kevany B M, Tieman D M, Taylor M G, Cin V D, Klee H J. 2007.Ethylene receptor degradation controls the timing ofripening in tomato fruit. The Plant Journal, 51, 458-467

[22]Kieber J J, Rothenberg M, Roman G, Feldmann K A, EckerJ R. 1993. CTR1, a negative regulator of the ethyleneresponse pathway in Arabidopsis, encodes a memberof the raf family of protein kinases. Cell, 72, 427-441

[23]Kim H, Helmbrecht E E, Stalans M B, Schmitt C, Patel N,Wen C K, Wang W Y, Binder B M. 2011. Ethylenereceptor ETHYLENE RECEPTOR1 domain requirementsfor ethylene responses in Arabidopsis seedlings. PlantPhysiology, 156, 417-429

[24]Klee H J. 2002. Control of ethylene-mediated processes intomato at the level of receptors. Journal of ExperimentalBotany, 53, 2057-2063

[25]Kuang J F, Chen J Y, Luo M, Wu K Q, Sun W, Jiang Y M,Lu W J. 2012. Histone deacetylase HD2 interacts withERF1 and is involved in longan fruit senescence.Journal of Experimental Botany, 63, 441-454

[26]Kuroda S, Hirose Y, Shiraishi M, Davies E, Abe S. 2004. Coexpressionof an ethylene receptor gene, ERS1, andethylene signaling regulator gene, CTR1, in Delphiniumduring abscission of florets. Plant Physiol Biochemistry,42, 745-751

[27]Lashbrook C C, Tieman D M, Klee H J. 1998. Differentialregulation of the tomato ETR gene family throughoutplant development. The Plant Journal, 15, 243-252

[28]Lin Z F, Arciga-Reyes L, Zhong S L, Alexander L, HackettR, Wilson I, Grierson D. 2008. SlTPR1, a tomatotetratricopeptide repeat protein, interacts with theethylene receptors NR and LeETR1, modulatingethylene and auxin responses and development.Journal of Experimental Botany, 59, 4271-4287

[29]Lin Z, Ho C W, Grierson D. 2009. AtTRP1 encodes a novelTPR protein that interacts with the ethylene receptorERS1 and modulates development in Arabidopsis.Journal of Experimental Botany, 60, 3697-3714

[30]Llop-Tous I, Barry C S, Grierson D. 2000. Regulation ofethylene biosynthesis in response to pollination intomato flowers. Plant Physiology, 123, 971-978

[31]Lohar D, Stiller J, Kam J, Stacey G, Gresshoff P M. 2009.Ethylene insensitivity conferred by a mutatedArabidopsis ethylene receptor gene alters nodulationin transgenic Lotus japonicus. Annals of Botany, 104,277-285

[32]Okuda M, Nang M P S H, Oshima K, Ishibashi Y, Zheng SH, Yuasa T, Iwaya-Inoue M. 2011. The ethylene signalmediates induction of GmATG8i in soybean plantsunder starvation stress. Bioscience Biotechnology andBiochemistry, 75, 1408-1412

[33]Osnato M, Stile M R, Wang Y M, Meynard D, Curiale S,Guiderdoni E, Liu Y X, Horner D S, Ouwerkerk P B F,Pozzi C, et al. 2010. Cross talk between the KNOX andethylene pathways is mediated by intron-bindingtranscription factors in barley. Plant Physiology, 154,1616-1632

[34]Qu X, Schaller G E. 2004. Requirement of the histidine kinasedomain for signal transduction by the ethylene receptorETR1. Plant Physiology, 136, 2961-2970

[35]Santner A, Estelle M. 2009. Recent advances and emergingtrends in plant hormone signalling. Nature, 459, 1071-1078

[36]Schaller G E. 2012. Ethylene and the regulation of plantdevelopment. BMC Biology, 10, 9.Shan X, Yan J, Xie D. 2012. Comparison of phytohormonesignaling mechanisms. Current Opinion in Plant Biology,15, 84-91

[37]Sikorski R S, Boguski M S, Goebl M, Hieter P. 1990. Arepeating amino acid motif in CDC23 defines a family ofproteins and a new relationship among genes requiredfor mitosis and RNA synthesis. Cell, 60, 307-317

[38]Stepanova A N, Alonso J M. 2009. Ethylene signaling andresponse: where different regulatory modules meet.Current Opinion in Plant Biology, 12, 548-555

[39]Wang K L C, Li H, Ecker J R. 2002. Ethylene biosynthesisand signaling networks. The Plant Cell, 14, S131-S151.Wang W, Hall A E, O’Malley R, Bleecker A B. 2003.Canonical histidine kinase activity of the transmitterdomain of the ETR1 ethylene receptor from Arabidopsisis not required for signal transmission. Proceedings ofthe National Academy of Sciences of the United Statesof America, 100, 352-357

[40]Wuriyanghan H, Zhang B, Cao W H, Ma B, Lei G, Liu Y F,Wei W, Wu H J, Chen L J, Chen H W, et al. 2009. Theethylene receptor ETR2 delays floral transition andaffects starch accumulation in rice. The Plant Cell, 21,1473-1494

[41]Xie Z M, Lei G, Hada W, Tian A G, Zhang J S, Chen S Y.2007. Cloning and expression of putative ethylenereceptor genes in soybean plant. Progress in NaturalScience, 17, 1152-1160

[42]Xu Z S, Ni Z Y, Li Z Y, Li L C, Chen M, Gao D Y, Yu X D,Liu P, Ma Y Z. 2009. Isolation and functionalcharacterization of HvDREB1 - a gene encoding adehydration-responsive element binding protein inHordeum vulgare. Journal of Plant Research, 122,121-130

[43]Xu Z S, Xia L Q, Chen M, Cheng X G, Zhang R Y, Li L C,Zhao Y X, Lu Y, Ni Z Y, Liu L, et al. 2007. Isolation andmolecular characterization of the Triticum aestivum L.ethylene-responsive factor 1 (TaERF1) that increasesmultiple stress tolerance. Plant Molecular Biology, 65,719-732

[44]Zhang G, Chen M, Chen X, Xu Z, Li L, Guo J, Ma Y. 2010.Isolation and characterization of a novel EAR-motifcontaininggene GmERF4 from soybean (Glycine max L.).Molecular Biology Reports, 37, 809-818.

Characterization of Ethylene Receptors and Their Interactions with GmTPRA Novel Tetratricopeptide Repeat Protein (TPR) in Soybean (Glycine max L.)

Characterization of Ethylene Receptors and Their Interactions with GmTPRA Novel Tetratricopeptide Repeat Protein (TPR) in Soybean (Glycine max L.)

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	KONG Ling-an, WU Du-qing, HUANG Wen-kun, PENG Huan, HE Wen-ting, PENG De-liang. Assaying the potential of twenty-one legume plants in Medicago truncatula and M. sativa for candidate model plants for investigation the interactions with Heterodera glycines[J]. Journal of Integrative Agriculture, 2016, 15(3): 702-704.
[2]	LI Yan-fei, HONG Hui-long, LI Ying-hui, MA Yan-song, CHANG Ru-zhen, QIU Li-juan. The identification of presence/absence variants associated with the apparent differences of growth period structures between cultivated and wild soybeans[J]. Journal of Integrative Agriculture, 2016, 15(2): 262-270.
[3]	ZHANG Li-wei, Til Feike, Jirko Holst, Christa Hoffmann, Reiner Doluschitz. Comparison of energy consumption and economic performance of organic and conventional soybean production - A case study from Jilin Province, China[J]. Journal of Integrative Agriculture, 2015, 14(8): 1561-1572.
[4]	GUO Bing-fu, GUO Yong, WANG Jun, ZHANG Li-juan, JIN Long-guo, HONG Hui-long, CHANG, Ru-zheng, QIU Li-juan. Co-treatment with surfactant and sonication significantly improves Agrobacterium-mediated resistant bud formation and transient expression efficiency in soybean[J]. Journal of Integrative Agriculture, 2015, 14(7): 1242-1250.
[5]	ZHOU De, Dieter Koemle. Price transmission in hog and feed markets of China[J]. Journal of Integrative Agriculture, 2015, 14(6): 1122-1129.
[6]	CHEN Hua-tao, CHEN Xin, WU Bing-yue, YUAN Xing-xing, ZHANG Hong-mei, CUI Xiao-yan. Whole-genome identification and expression analysis of K+ efflux antiporter (KEA) and Na+/H+ antiporter (NHX) families under abiotic stress in soybean[J]. Journal of Integrative Agriculture, 2015, 14(6): 1171-1183.
[7]	WANG Xiao-guang, ZHAO Xin-hua, JIANG Chun-ji, LI Chun-hong, CONG Shan, WU Di, CHEN Yan-qiu, YU Hai-qiu, WANG Chun-yan. Effects of potassium deficiency on photosynthesis and photoprotection mechanisms in soybean (Glycine max (L.) Merr.)[J]. Journal of Integrative Agriculture, 2015, 14(5): 856-863.
[8]	WU Ting-ting, LI Jin-yu, WU Cun-xiang, SUN Shi, MAO Ting-ting, JIANG Bing-jun, HOU Wen-sheng, HAN Tian-fu. Analysis of the independent- and interactive-photo-thermal effects on soybean flowering[J]. Journal of Integrative Agriculture, 2015, 14(4): 622-632.
[9]	MA Ya-nan, CHEN Ming, XU Dong-bei, FANG Guang-ning, WANG Er-hui, GAO Shi-qing, XU Zhao-shi, LI Lian-cheng, ZHANG Xiao-hong, MIN Dong-hong, MA You-zhi. G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis[J]. Journal of Integrative Agriculture, 2015, 14(2): 314-325.
[10]	WEI Fang, HU Jie, YANG Yan, HAO Zhi-da, WU Rui-hua, TIAN Bao-ming, CAO Gang-qiang, ZANG Xin. Transgenic Arabidopsis thaliana expressing a wheat oxalate oxidase exhibits hydrogen peroxide related defense response[J]. Journal of Integrative Agriculture, 2015, 14(12): 2565-2573.
[11]	WANG Jun, LIU Lin, GUO Yong, WANG Yong-hui, ZHANG Le, JIN Long-guo, GUAN Rong-xia, LIU Zhang-xiong, WANG Lin-lin, CHANG Ru-zhen , QIU Li-juan. A Dominant Locus, qBSC-1, Controls β Subunit Content of Seed Storage Protein in Soybean (Glycine max (L.) Merri.)[J]. Journal of Integrative Agriculture, 2014, 13(9): 1854-1864.
[12]	LIJian-ping, HOUPei, ZHENGXu, SONGMei-fang, SULiang, YANGJian-ping. Arabidopsis Phytochrome D Is Involved in Red Light-Induced Negative Gravitropism of Hypocotyles[J]. Journal of Integrative Agriculture, 2014, 13(8): 1634-1639.
[13]	AO Xue, GUO Xiao-hong, ZHU Qian, ZHANG Hui-jun, WANG Hai-ying, MA Zhao-hui, HAN, Xiao-ri, ZHAO Ming-hui , XIE Fu-ti. Effect of Phosphorus Fertilization to P Uptake and Dry Matter Accumulation in Soybean with Different P Efficiencies[J]. Journal of Integrative Agriculture, 2014, 13(2): 326-334.
[14]	ZHANG Gu-wen, XU Sheng-chun, HU Qi-zan, MAO Wei-hua , GONG Ya-ming. Putrescine Plays a Positive Role in Salt-Tolerance Mechanisms by Reducing Oxidative Damage in Roots of Vegetable Soybean[J]. Journal of Integrative Agriculture, 2014, 13(2): 349-357.
[15]	LI Xi-huan, WANG Yun-jie, WU Bing, KONG You-bin, LI Wen-long, CHANG Wen-suo , ZHANG Cai-ying. GmPHR1, a Novel Homolog of the AtPHR1 Transcription Factor, Plays a Role in Plant Tolerance to Phosphate Starvation[J]. Journal of Integrative Agriculture, 2014, 13(12): 2584-2593.