Characterization of Tomato Transcription Factor WUSCHEL and Functional Study in Arabidopsis

doi:10.1016/S1671-2927(00)8654

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (8): 1257-1265.DOI: 10.1016/S1671-2927(00)8654

Characterization of Tomato Transcription Factor WUSCHEL and Functional Study in Arabidopsis

WANG Xiang, WANG Xin-guo, REN Jiang-ping, MA Ying, YIN Jun

1.National Center for Wheat Research, Henan Agricultural University, Zhengzhou 450002, P.R.China
2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2011-05-31 出版日期:2012-08-01 发布日期:2012-09-09
通讯作者: Correspondence YIN Jun, Tel: +86-371-63558203, E-mail: xmzxyj@126.com, wxgyz@sohu.com
作者简介:WANG Xiang, Tel: +86-371-63558215, E-mail: wxgyz@sohu.com
基金资助:
This research was supported by the National Natural Science Foundation of China (30670188), the National Key Project for Researches on Transgenic Plant of China (2009ZX08002-011B) and the Education Department Project Of Henan Province, China (2010B210015).

Characterization of Tomato Transcription Factor WUSCHEL and Functional Study in Arabidopsis

WANG Xiang, WANG Xin-guo, REN Jiang-ping, MA Ying, YIN Jun

1.National Center for Wheat Research, Henan Agricultural University, Zhengzhou 450002, P.R.China
2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2011-05-31 Online:2012-08-01 Published:2012-09-09
Contact: Correspondence YIN Jun, Tel: +86-371-63558203, E-mail: xmzxyj@126.com, wxgyz@sohu.com
About author:WANG Xiang, Tel: +86-371-63558215, E-mail: wxgyz@sohu.com
Supported by:
This research was supported by the National Natural Science Foundation of China (30670188), the National Key Project for Researches on Transgenic Plant of China (2009ZX08002-011B) and the Education Department Project Of Henan Province, China (2010B210015).

摘要/Abstract

摘要： The homeobox transcription factor WUSCHEL (WUS) plays a critical role in keeping the balance between the maintenance and differentiation of stem cell population in shoot and floral meristems of Arabidopsis thaliana. The corresponding gene SlWUS is yet to be characterized in tomato. In order to characterize SlWUS gene and its biological function, we cloned it from tomato and analyzed its structure. Tissue expression showed that the SlWUS highly expressed in tomato flower abscission zone. The overexpression of SlWUS in Arabidopsis could trigger undifferentiation of plant flower organ and indeterminacy of flower identity, suggesting that SlWUS maybe involved in flower structure development as well as flower organ identity. Taken together, our results indicated that the SlWUS plays an important role in flower abscission zone and plant organ shedding.

关键词: SlWUS, tomato, meristem, determination, Arabidopsis , Received

Abstract: The homeobox transcription factor WUSCHEL (WUS) plays a critical role in keeping the balance between the maintenance and differentiation of stem cell population in shoot and floral meristems of Arabidopsis thaliana. The corresponding gene SlWUS is yet to be characterized in tomato. In order to characterize SlWUS gene and its biological function, we cloned it from tomato and analyzed its structure. Tissue expression showed that the SlWUS highly expressed in tomato flower abscission zone. The overexpression of SlWUS in Arabidopsis could trigger undifferentiation of plant flower organ and indeterminacy of flower identity, suggesting that SlWUS maybe involved in flower structure development as well as flower organ identity. Taken together, our results indicated that the SlWUS plays an important role in flower abscission zone and plant organ shedding.

Key words: SlWUS, tomato, meristem, determination, Arabidopsis , Received

WANG Xiang, WANG Xin-guo, REN Jiang-ping, MA Ying, YIN Jun. Characterization of Tomato Transcription Factor WUSCHEL and Functional Study in Arabidopsis[J]. Journal of Integrative Agriculture, 2012, 12(8): 1257-1265.

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Characterization of Tomato Transcription Factor WUSCHEL and Functional Study in Arabidopsis

Characterization of Tomato Transcription Factor WUSCHEL and Functional Study in Arabidopsis

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