Molecular Characterization and Expression Analysis of TaZFP15, a C2H2- Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.)

doi:10.1016/S1671-2927(00)8521

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (1): 31-42.DOI: 10.1016/S1671-2927(00)8521

Molecular Characterization and Expression Analysis of TaZFP15, a C2H2- Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.)

SUN Zhao-hua, DING Chang-huan, LI Xiao-juan , XIAO Kai

1.College of Life Science, Agricultural University of Hebei, Baoding 071001, P.R.China
2.College of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China

收稿日期:2011-01-15 出版日期:2012-01-01 发布日期:2012-01-13
通讯作者: Correspondence XIAO Kai, Tel: +86-312-7528115, Fax: +86-312-7528400, E-mail: xiaokai@hebau.edu.cn; LI Xiao-juan, Tel: +86-312-7528245, Fax: +86-312-7528200, E-mail: lxjlixiaojuan@yahoo.com.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (30971773), the Natural Science Foundation of Hebei Province, China (C2011204031) and the Key Laboratory of Crop Growth Regulation of Hebei Province, China.

Molecular Characterization and Expression Analysis of TaZFP15, a C2H2- Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.)

SUN Zhao-hua, DING Chang-huan, LI Xiao-juan , XIAO Kai

1.College of Life Science, Agricultural University of Hebei, Baoding 071001, P.R.China
2.College of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China

Received:2011-01-15 Online:2012-01-01 Published:2012-01-13
Contact: Correspondence XIAO Kai, Tel: +86-312-7528115, Fax: +86-312-7528400, E-mail: xiaokai@hebau.edu.cn; LI Xiao-juan, Tel: +86-312-7528245, Fax: +86-312-7528200, E-mail: lxjlixiaojuan@yahoo.com.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (30971773), the Natural Science Foundation of Hebei Province, China (C2011204031) and the Key Laboratory of Crop Growth Regulation of Hebei Province, China.

摘要/Abstract

摘要： Based on sequencing of part clones in a root subtractive cDNA library, an expressed sequence tag (EST) sharing high similarity to a rice C2H2 zinc finger transcription factor (ZFP15) was obtained in wheat. Through bioinformatics approach, the wheat C2H2-type ZFP gene referred to TaZFP15 has been identified and characterized. As a full-length cDNA of 670 bp, TaZFP15 has an open reading frame of 408 bp and encodes a 135-aa polypeptide. TaZFP15 contains two C2H2 zinc finger domains and each one has a conserved motif QALGGH. The typical L-box, generally identified in the C2H2 type transcription factors, has also been found in TaZFP15. Phylogenetic analysis suggested that TaZFP15 shares high similarities with rice ZFP15 (GenBank accession no. AY286473), maize ZFP (GenBank accession no. NM_001159094) and a subset of other zinc-finger transcription factor genes in plant species. The expression of TaZFP15 was up-regulated by starved-Pi stress, showing a pattern to be gradually elevated along with the progression of the Pi-stress in a 23-h treatment regime. Similarly, the transcripts of TaZFP15 in roots were also induced by nitrogen deficiency, and abiotic stresses of drought and salinity. No responses of TaZFP15 were detected in roots to nutrition deficiencies of P, Zn, and Ca, and the external treatment of abscisic acid (ABA). TaZFP15 could be specifically amplified in genome A, B, and D, and without variability in the sequences, suggesting that TaZFP15 has multi-copies in the homologous hexaploid species. Transgenic analysis in tobacco revealed that up-regulation of TaZFP15 could significantly improve plant dry mass accumulation via increasing the plant phosphorus acquisition capacity under Pi-deficiency condition. The results suggested that TaZFP15 is involved in mediation of signal transductions of diverse external stresses.

关键词: Triticum aestivum L., zinc-finger transcription factor gene, nutrition deficiency, abiotic stress, expression pattern

Abstract: Based on sequencing of part clones in a root subtractive cDNA library, an expressed sequence tag (EST) sharing high similarity to a rice C2H2 zinc finger transcription factor (ZFP15) was obtained in wheat. Through bioinformatics approach, the wheat C2H2-type ZFP gene referred to TaZFP15 has been identified and characterized. As a full-length cDNA of 670 bp, TaZFP15 has an open reading frame of 408 bp and encodes a 135-aa polypeptide. TaZFP15 contains two C2H2 zinc finger domains and each one has a conserved motif QALGGH. The typical L-box, generally identified in the C2H2 type transcription factors, has also been found in TaZFP15. Phylogenetic analysis suggested that TaZFP15 shares high similarities with rice ZFP15 (GenBank accession no. AY286473), maize ZFP (GenBank accession no. NM_001159094) and a subset of other zinc-finger transcription factor genes in plant species. The expression of TaZFP15 was up-regulated by starved-Pi stress, showing a pattern to be gradually elevated along with the progression of the Pi-stress in a 23-h treatment regime. Similarly, the transcripts of TaZFP15 in roots were also induced by nitrogen deficiency, and abiotic stresses of drought and salinity. No responses of TaZFP15 were detected in roots to nutrition deficiencies of P, Zn, and Ca, and the external treatment of abscisic acid (ABA). TaZFP15 could be specifically amplified in genome A, B, and D, and without variability in the sequences, suggesting that TaZFP15 has multi-copies in the homologous hexaploid species. Transgenic analysis in tobacco revealed that up-regulation of TaZFP15 could significantly improve plant dry mass accumulation via increasing the plant phosphorus acquisition capacity under Pi-deficiency condition. The results suggested that TaZFP15 is involved in mediation of signal transductions of diverse external stresses.

Key words: Triticum aestivum L., zinc-finger transcription factor gene, nutrition deficiency, abiotic stress, expression pattern

SUN Zhao-hua, DING Chang-huan, LI Xiao-juan , XIAO Kai. Molecular Characterization and Expression Analysis of TaZFP15, a C2H2- Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.)[J]. Journal of Integrative Agriculture, 2012, 12(1): 31-42.

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Molecular Characterization and Expression Analysis of TaZFP15, a C2H2- Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.)

Molecular Characterization and Expression Analysis of TaZFP15, a C2H2- Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.)

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