Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (9): 1900-1908.DOI: 10.1016/S2095-3119(13)60518-3

• 论文 • 上一篇    下一篇

Transcriptional Regulation of Expression of the Maize Aldehyde Dehydrogenase 7 Gene (ZmALDH7B6) in Response to Abiotic Stresses

 AN Xia, DUAN Feng-ying, GUO Song, CHEN Fan-jun, YUAN Li-xing , GU Ri-liang   

  1. Department of Plant Nutrition, Center for Resources, Environment and Food Security, China Agricultural University, Beijing 100193, P.R.China
  • 收稿日期:2013-03-15 出版日期:2014-09-22 发布日期:2014-09-24
  • 通讯作者: GU Ri-liang, Tel: +86-10-62734424, Fax: +86-10-62731016, E-mail: rilianggu@cau.edu.cn
  • 作者简介:AN Xia, E-mail: anxia0217@163.com
  • 基金资助:

    This work was financially supported by the National 863 Program of China (2012AA100306), the National 973 Program of China (2011CB100305), the National Natural Science Foundation of China (30971863, 31121062), and the Ministry of Agriculture of China (2011ZX08003-005).

Transcriptional Regulation of Expression of the Maize Aldehyde Dehydrogenase 7 Gene (ZmALDH7B6) in Response to Abiotic Stresses

 AN Xia, DUAN Feng-ying, GUO Song, CHEN Fan-jun, YUAN Li-xing , GU Ri-liang   

  1. Department of Plant Nutrition, Center for Resources, Environment and Food Security, China Agricultural University, Beijing 100193, P.R.China
  • Received:2013-03-15 Online:2014-09-22 Published:2014-09-24
  • Contact: GU Ri-liang, Tel: +86-10-62734424, Fax: +86-10-62731016, E-mail: rilianggu@cau.edu.cn
  • About author:AN Xia, E-mail: anxia0217@163.com
  • Supported by:

    This work was financially supported by the National 863 Program of China (2012AA100306), the National 973 Program of China (2011CB100305), the National Natural Science Foundation of China (30971863, 31121062), and the Ministry of Agriculture of China (2011ZX08003-005).

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摘要： Aldehyde dehydrogenases (ALDHs) represent a large protein family, which includes several members that catalyze the oxidation of an aldehyde to its corresponding carboxylic acid in plants. Genes encoding members of the ALDH7 subfamily have been suggested to play important roles in various stress adaptations in plants. In this study, quantitative RT-PCR analysis revealed that a maize ALDH7 subfamily member (ZmALDH7B6) was constitutively expressed in various organs, including roots, leaves, immature ears, tassels, and developing seeds. The abundance of ZmALDH7B6 mRNA transcripts in maize roots was increased by ammonium, NaCl, and mannitol treatments. To further analyze tissue-specific and stress-induced expression patterns, the 1.5-kb 5´-flanking ZmALDH7B6 promoter region was fused to the β-glucuronidase (GUS) reporter gene and introduced into maize plants. In roots of independent transgenic lines, there was significant induction of GUS activity in response to ammonium supply, confirming ammonium-dependent expression of ZmALDH7B6 at the transcript level. Histochemical staining showed that GUS activity driven by the ZmALDH7B6 promoter was mainly localized in the vascular tissues of maize roots. These results suggested that ZmALDH7B6 is induced by multiple environmental stresses in maize roots, and may play a role in detoxifying aldehydes, particularly in vascular tissue.

关键词: abiotic stress , aldehyde dehydrogenase , gene expression , promoter , transgenic maize

Abstract: Aldehyde dehydrogenases (ALDHs) represent a large protein family, which includes several members that catalyze the oxidation of an aldehyde to its corresponding carboxylic acid in plants. Genes encoding members of the ALDH7 subfamily have been suggested to play important roles in various stress adaptations in plants. In this study, quantitative RT-PCR analysis revealed that a maize ALDH7 subfamily member (ZmALDH7B6) was constitutively expressed in various organs, including roots, leaves, immature ears, tassels, and developing seeds. The abundance of ZmALDH7B6 mRNA transcripts in maize roots was increased by ammonium, NaCl, and mannitol treatments. To further analyze tissue-specific and stress-induced expression patterns, the 1.5-kb 5´-flanking ZmALDH7B6 promoter region was fused to the β-glucuronidase (GUS) reporter gene and introduced into maize plants. In roots of independent transgenic lines, there was significant induction of GUS activity in response to ammonium supply, confirming ammonium-dependent expression of ZmALDH7B6 at the transcript level. Histochemical staining showed that GUS activity driven by the ZmALDH7B6 promoter was mainly localized in the vascular tissues of maize roots. These results suggested that ZmALDH7B6 is induced by multiple environmental stresses in maize roots, and may play a role in detoxifying aldehydes, particularly in vascular tissue.

Key words: abiotic stress , aldehyde dehydrogenase , gene expression , promoter , transgenic maize