Expression analysis for candidate genes associated with eggshell mechanical property

doi:10.1016/S2095-3119(14)60969-2

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (2): 397-402.DOI: 10.1016/S2095-3119(14)60969-2

Expression analysis for candidate genes associated with eggshell mechanical property

SUN Cong-jiao, DUaN Zhong-yi, QU Lu-jiang, ZhENg Jiang-xia, YaNg Ning, XU gui-yun

National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture/ College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China

收稿日期:2014-12-03 出版日期:2016-02-05 发布日期:2016-02-01
通讯作者: XU Gui-yun, Mobile: +86-13601372958,Fax: +86-10-62732741, E-mail: ncppt@cau.edu.cn
作者简介:SUN Cong-jiao, Mobile: +86-15910707895, E-mail: suncongjiao@163.com
基金资助:
The current research was funded by the National Natural Science Foundation of China (31472084), the National High Technology Research and Development Program of China (2013AA102501), the National Key Technology R&D Program of China (2011BAD28B03), the Programs for Changjiang Scholars and Innovative Research in University, China (IRT1191), and the China Agriculture Research Systems (CARS-41).

Expression analysis for candidate genes associated with eggshell mechanical property

SUN Cong-jiao, DUaN Zhong-yi, QU Lu-jiang, ZhENg Jiang-xia, YaNg Ning, XU gui-yun

National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture/ College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China

Received:2014-12-03 Online:2016-02-05 Published:2016-02-01
Contact: XU Gui-yun, Mobile: +86-13601372958,Fax: +86-10-62732741, E-mail: ncppt@cau.edu.cn
About author:SUN Cong-jiao, Mobile: +86-15910707895, E-mail: suncongjiao@163.com
Supported by:
The current research was funded by the National Natural Science Foundation of China (31472084), the National High Technology Research and Development Program of China (2013AA102501), the National Key Technology R&D Program of China (2011BAD28B03), the Programs for Changjiang Scholars and Innovative Research in University, China (IRT1191), and the China Agriculture Research Systems (CARS-41).

摘要/Abstract

摘要： Damaged eggshells result in losses of eggs. The genetic mechanism of variable eggshell strength is still unclear. The current study was conducted to verify whether the eggshell calcification related genes, CALB1, SPP1, DMP4, BMP2 and SLIT2, were associated with eggshell mechanical property. For this purpose quantitative PCR (q-PCR) analysis was performed to detect gene expression between two groups of hens laying strong and weak eggs. The hens were selected from 360 White Leghorn layers at 60 wk to ensure that the strong and weak eggs differed significantly in breaking strength but not in eggshell thickness and weight. Using this special strong/weak eggshell model, we found that the expression of CALB1 in the uterus of strong shell group was about 3-fold higher (P<0.05) than that in weak shell group. The DMP4 expression was significantly higher (2-fold, P<0.05) in the uterus of weak shell group than that in strong shell group. However, no difference was observed for genes of SPP1, SLIT2 and BMP2 between these two groups. The current study provides a new insight to investigate the association of candidate genes with eggshell mechanical property.

关键词: eggshell mechanical property , eggshell calcification related genes , q-PCR , chicken

Abstract: Damaged eggshells result in losses of eggs. The genetic mechanism of variable eggshell strength is still unclear. The current study was conducted to verify whether the eggshell calcification related genes, CALB1, SPP1, DMP4, BMP2 and SLIT2, were associated with eggshell mechanical property. For this purpose quantitative PCR (q-PCR) analysis was performed to detect gene expression between two groups of hens laying strong and weak eggs. The hens were selected from 360 White Leghorn layers at 60 wk to ensure that the strong and weak eggs differed significantly in breaking strength but not in eggshell thickness and weight. Using this special strong/weak eggshell model, we found that the expression of CALB1 in the uterus of strong shell group was about 3-fold higher (P<0.05) than that in weak shell group. The DMP4 expression was significantly higher (2-fold, P<0.05) in the uterus of weak shell group than that in strong shell group. However, no difference was observed for genes of SPP1, SLIT2 and BMP2 between these two groups. The current study provides a new insight to investigate the association of candidate genes with eggshell mechanical property.

Key words: eggshell mechanical property , eggshell calcification related genes , q-PCR , chicken

SUN Cong-jiao, DUaN Zhong-yi, QU Lu-jiang, ZhENg Jiang-xia, YaNg Ning, XU gui-yun. Expression analysis for candidate genes associated with eggshell mechanical property[J]. Journal of Integrative Agriculture, 2016, 15(2): 397-402.

参考文献

Ahmed A M H, Rodriguez-Navarro A B, Vidal M L, GautronJ, García-Ruiz J M, Nys Y. 2005. Changes in eggshellmechanical properties, crystallographic texture and in matrixproteins induced by moult in hens. British Poultry Science,46, 268-279

Arazi H, Yoselewitz I, Malka Y, Kelner Y, Genin O, Pines M.2009. Osteopontin and calbindin gene expression in theeggshell gland as related to eggshell abnormalities. PoultryScience, 88, 647-653

Bain M M. 1990. Eggshell strength: A mechanical ultrastructuralevaluation. Ph D thesis, Faculty of Veterinary Medicine,University of Glasgow, UK.

Bar A, Striem S, Vax E, Talpaz H, Hurwitz S. 1992a. Regulationof calbindin mRNA and calbindin turnover in intestine andshell gland of the chicken. American Journal of Physiology,262, 800-805

Bar A, Vax E, Striem S. 1992b. Relationships between calbindin(Mr 28,000) and calcium transport by the eggshell gland.Comparative Biochemistry and Physiology, 101, 845-848

Battye R, Stevens A, Perry R L, Jacobs J R. 2001. Repellentsignaling by slit requires the leucine-rich repeats. TheJournal of Neuroscience, 21, 4290-4298

Chien Y C, Hincke M, Vali H, McKee M. 2008. Ultrastructuralmatrix-mineral relationships in avian eggshell, and effects ofosteopontin on calcite growth in vitro. Journal of StructuralBiology, 163, 84-99

Chien Y C, Hincke M T, McKee M D. 2009. Ultrastructure ofavian eggshell during resorption following egg fertilization.Journal of Structural Biology, 168, 527-538

Duprez D, Coltey M, Amthor H, Brickell P, Tickle C. 1996.Bone morphogenetic protein-2 (BMP-2) inhibits muscledevelopment and promotes cartilage formation in chicklimb bud cultures. Developmental Biology, 174, 448-452

Gautron J, Hincke M T, Nys Y. 1997. Precursor matrix proteinsin the uterine fluid change with stages of eggshell formationin hens. Connective Tissue Research, 36, 195-210

Hao J, Narayanan K, Muni T, Ramachandran A, George A.2007. Dentin matrix protein 4, a novel secretory calciumbindingprotein that modulates odontoblast differentiation.The Journal of Biological Chemistry, 282, 15357-15365

Hincke M T, Chien Y C, Gerstenfeld L C, McKee M D.2008. Colloidal-gold immunocytochemical localization ofosteopontin in avian eggshell gland and eggshell. Journalof Histochemistry & Cytochemistry, 56, 467-476

Jeong W, Lim W, Kim J, Ahn S E, Lee H C, Jeong J W,Han J Y, Song G, Bazer F W. 2012. Cell-specific andtemporal aspects of gene expression in the chickenoviduct at different stages of the laying cycle 1. Biology ofReproduction, 86, 172-180

Jonchere V, Brionne A, Gautron J, Nys Y. 2012. Identificationof uterine ion transporters for mineralisation precursors ofthe avian eggshell. BMC Physiology, 12, 10.

Jonchere V, Rehault-Godbert S, Hennequet-Antier C, CabauC, Sibut V, Cogburn L, Nys Y, Gautron J. 2010. Geneexpression profiling to identify eggshell proteins involvedin physical defense of the chicken egg. BMC Genomics,11, 57.

Kobe B, Deisenhofer J. 1993. Crystal structure of porcineribonuclease inhibitor, a protein with leucine-rich repeats.Nature, 366, 751-756

Lavelin I, Yarden N, Ben-Bassat S, Bar A, Pines M. 1998.Regulation of osteopontin gene expression during egg shellformation in the laying hen by mechanical strain. MatrixBiology, 17, 615-623

Liu Z, Zheng Q, Zhang X, Lu L. 2013. Microarray analysisof genes involved with shell strength in layer shell gland atthe early stage of active calcification. Asian-AustralasianJournal of Animal Sciences, 26, 609-624

Nys Y, Gautron J, Garcia-Ruiz J M, Hincke M T. 2004. Avianeggshell mineralization: Biochemical and functionalcharacterization of matrix proteins. Comptes RendusPalevol, 3, 549-562

Nys Y, Hincke M, Arias J, Garcia-Ruiz J, Solomon S. 1999.Avian eggshell mineralization. Poultry and Avian BiologyReviews, 10, 143-166

Nys Y, Mayel-Afshar S, Bouillon R, Van Baelen H, Lawson D.1989. Increases in calbindin D 28K mRNA in the uterusof the domestic fowl induced by sexual maturity and shellformation. General and Comparative Endocrinology, 76, 322-329

Panheleux M, Nys Y, Williams J, Gautron J, Boldicke T, HinckeM T. 2000. Extraction and quantification by ELISA ofeggshell organic matrix proteins (ovocleidin-17, ovalbumin,ovotransferrin) in shell from young and old hens. PoultryScience, 79, 580-588

Pines M. 2007. The involvement of matrix proteins in eggshellformation. In: Proceedings of the 19th Australian PoultryScience Symposium. Sydney, New South Wales, Australia.pp. 130-133

Pines M, Knopov V, Bar A. 1995. Involvement of osteopontinin egg shell formation in the laying chicken. Matrix Biology,14, 765-771

Preisinger R, Flock D K. 2000. Genetic Changes in LayerBreeding: Historical Trends and Future Prospects. BSASOccasional Publication, UK. pp. 20-28

Rodriguez-Navarro A, Kalin O, Nys Y, Garcia-Ruiz J M. 2002.Influence of the microstructure on the shell strength of eggslaid by hens of different ages. British Poultry Science, 43,395-403

Sekiya I, Larson B L, Vuoristo J T, Reger R L, Prockop D J.2005. Comparison of effect of BMP-2, -4, and -6 on in vitrocartilage formation of human adult stem cells from bonemarrow stroma. Cell and Tissue Research, 320, 269-276

Sun C, Xu G, Yang N. 2013. Differential label-free quantitativeproteomic analysis of avian eggshell matrix and uterinefluid proteins associated with eggshell mechanical property.Proteomics, 13, 3523-3536

Yang J, Zhao Z, Hou J, Zhou Z, Deng Y, Dai J. 2013. Expressionof TRPV6 and CaBP-D28k in the egg shell gland (uterus)during the oviposition cycle of the laying hen. British PoultryScience, 54, 398–406.

[1]	ZHANG Wen-wu, KONG Li-na, ZHANG De-xiang, JI Cong-liang, ZHANG Xi-quan, LUO Qing-bin. Effect of the C.–1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance[J]. Journal of Integrative Agriculture, 2015, 14(9): 1808-1815.
[2]	ZHANG Lei, ZUO Qi-sheng, LI Dong, LIAN Chao, Kamel E Ahmed, TANG Bei-bei, SONG Jiu-zhou, ZHANG Ya-ni, LI Bi-chun. Study on the role of JAK/STAT signaling pathway during chicken spermatogonial stem cells generation based on RNA-Seq[J]. Journal of Integrative Agriculture, 2015, 14(5): 939-948.
[3]	Zhao Guang-wei, WanG Shuai, WanG Wang, ZhanG Zhen-chao, XIe Qing, ZhanG Meng, I a hassan, Yan Ruo-feng, SonG Xiao-kai, Xu Li-xin, LI Xiang-rui. Type I strain of Toxoplasma gondii from chicken induced different immune responses with that from human, cat and swine in chicken[J]. Journal of Integrative Agriculture, 2015, 14(5): 956-965.
[4]	LI Ling-ling , LI Shu-tian. Nitrogen Mineralization from Animal Manures and Its Relation to Organic N Fractions[J]. Journal of Integrative Agriculture, 2014, 13(9): 2040-2048.
[5]	ZHOU Zuo-yong, HU Shi-jun, WANG Zhi-ying, GUO Zhi-li, QIN Bo , NIE Kui. Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella[J]. Journal of Integrative Agriculture, 2014, 13(4): 904-910.
[6]	YANG Xiu-rong, JIANG He-sheng, ZHENG Jiang-xia, QU Lu-jiang, CHEN Si-rui, LI Jun-ying, XU Guiyun, YANG Ning. Dosage Effects of Fadrozole on Growth and Development of Sex-Reversed Genetic Female Chickens[J]. Journal of Integrative Agriculture, 2013, 12(6): 1049-1053.
[7]	FANG Li-xiu, XIN Rui, CHEYi, XU Shi-qing. Expression of Sex-RelatedGenes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol[J]. Journal of Integrative Agriculture, 2013, 12(1): 127-135.
[8]	OUYANG Jian-hua, XIE Liang, NIE Qing-hua, ZENG Hua, PENG Zhi-jun, ZHANG De-xiang, ZHANG Xi-quan. The Effects of Different Sex-Linked Dwarf Variations on Chinese Native Chickens[J]. Journal of Integrative Agriculture, 2012, 12(9): 1500-1508.
[9]	ZHAO Guang-wei, SHEN Bo, XIE Qing, XU Li-xin, YAN Ruo-feng, SONG Xiao-kai, Hassan Ibrahim Adam, LI Xiang-rui. Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China[J]. Journal of Integrative Agriculture, 2012, 12(8): 1347-1353.
[10]	SUN Gui-rong, LI Yan, KANG Xiang-tao, TIAN Ya-dong, ZHANG Hu , LI Kui. Effect of Beak Trimming Stress on the Apoptosis and Its Related Protein Expression of Chicken Spleen[J]. Journal of Integrative Agriculture, 2012, 12(4): 639-645.
[11]	ZHU Jia-ting, FENG Min, YAN Jian-min, LIU Chun-quan, HA Yi-ming, GAO Mei-xu, YANG Ping, WANG Zhi-dong, WANG De-ning, LI Shu-rong, GU Gui-qiang. Toxicological Evaluation of Chicken-Breast Meat with High-Dose Irradiation[J]. Journal of Integrative Agriculture, 2012, 12(12): 2088-2096.
[12]	CHEN Juan, MA Hai-tian, WANG Man, KONG Yi-li , ZOU Si-xiang. Creatine Pyruvate Enhances Lipolysis and Protein Synthesis in Broiler Chicken[J]. Journal of Integrative Agriculture, 2011, 10(12): 1977-1985.

Expression analysis for candidate genes associated with eggshell mechanical property

Expression analysis for candidate genes associated with eggshell mechanical property

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics