Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (7): 1398-1406.DOI: 10.1016/S2095-3119(14)60923-0

• 论文 • 上一篇    下一篇

Expression patterns of OCT4, NANOG, and SOX2 in goat preimplantation embryos from in vivo and in vitro

 YU Xiao-li, ZHAO Xiao-e, WANG Hua-yan, MA Bao-hua   

  1. 1、College of Veterinary Medicine, Northwest A&F University, Yangling 712100, P.R.China
    2、Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Yangling 712100, P.R.China
  • 收稿日期:2014-07-30 出版日期:2015-07-03 发布日期:2015-07-06
  • 通讯作者: MABao-hua, Tel: +86-29-87091117, Fax: +86-29-87091032,E-mail: mabh@nwsuaf.edu.cn
  • 作者简介:YU Xiao-li, E-mail: yuxiaoli1221@gmail.com;
  • 基金资助:

    This work was supported by the Genetically Modified Organisms Breeding Major Projects, Ministry of Agriculture, China (2008ZX0810-001).

Expression patterns of OCT4, NANOG, and SOX2 in goat preimplantation embryos from in vivo and in vitro

 YU Xiao-li, ZHAO Xiao-e, WANG Hua-yan, MA Bao-hua   

  1. 1、College of Veterinary Medicine, Northwest A&F University, Yangling 712100, P.R.China
    2、Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Yangling 712100, P.R.China
  • Received:2014-07-30 Online:2015-07-03 Published:2015-07-06
  • Contact: MABao-hua, Tel: +86-29-87091117, Fax: +86-29-87091032,E-mail: mabh@nwsuaf.edu.cn
  • About author:YU Xiao-li, E-mail: yuxiaoli1221@gmail.com;
  • Supported by:

    This work was supported by the Genetically Modified Organisms Breeding Major Projects, Ministry of Agriculture, China (2008ZX0810-001).

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摘要： The transcription factors, including OCT4, NANOG, and SOX2, played crucial roles in the maintenance of self-renewal and pluripotency in embryonic stem cells (ESCs). They expressed in preimplantation mammalian development with spatio- temporal pattern and took part in regulation of development. However, their expression and roles in goat had not been reported. In the present study, the expression of OCT4, NANOG, and SOX2 in goat preimplantation embryos both in vivo and in vitro were detected by real-time RCR and immunofluorescence. For in vivo fertilized embryos, the transcripts of OCT4, NANOG, and SOX2 could be detected from oocytes to blastocyst stage, their expression in morula and blastocyst stages was much higher than other stage. OCT4 protein was detected from oocyte to blastocyst, but the fluorescence was more located-intensive with nuclei from 8-cell stage, its expression present in both inner cell mass (ICM) and trophoblast cells (TE) at blastocyse stage. NANOG protein was similar to OCT4, the signaling of fluorescence completely focused on cell nuclei, while the SOX2 firstly showed nuclei location in morula. Comparing to in vivo fertilized embryo, the mRNA of these three transcription factors could be detected at 8-cell stage in parthenogenetic embryos (in vitro). Thereafter, the expressional level rose gradually along with embryo development. The locations of OCT4 and NANOG proteins were similar to in vivo fertilized embryos, and they located in cell nuclei from morula to blastocyst stage, while SOX2 protein firstly could be detected in cell nuclei at 8-cell stage. These differences suggested that OCT4, NANOG, and SOX2 played different function in regulating development of goat preimplantation embryos. These results may provide a novel insight to goat embryo development and be useful for goat ESCs isolation.

关键词: goat , OCT4 , NANOG , SOX2 , embryo , expression pattern

Abstract: The transcription factors, including OCT4, NANOG, and SOX2, played crucial roles in the maintenance of self-renewal and pluripotency in embryonic stem cells (ESCs). They expressed in preimplantation mammalian development with spatio- temporal pattern and took part in regulation of development. However, their expression and roles in goat had not been reported. In the present study, the expression of OCT4, NANOG, and SOX2 in goat preimplantation embryos both in vivo and in vitro were detected by real-time RCR and immunofluorescence. For in vivo fertilized embryos, the transcripts of OCT4, NANOG, and SOX2 could be detected from oocytes to blastocyst stage, their expression in morula and blastocyst stages was much higher than other stage. OCT4 protein was detected from oocyte to blastocyst, but the fluorescence was more located-intensive with nuclei from 8-cell stage, its expression present in both inner cell mass (ICM) and trophoblast cells (TE) at blastocyse stage. NANOG protein was similar to OCT4, the signaling of fluorescence completely focused on cell nuclei, while the SOX2 firstly showed nuclei location in morula. Comparing to in vivo fertilized embryo, the mRNA of these three transcription factors could be detected at 8-cell stage in parthenogenetic embryos (in vitro). Thereafter, the expressional level rose gradually along with embryo development. The locations of OCT4 and NANOG proteins were similar to in vivo fertilized embryos, and they located in cell nuclei from morula to blastocyst stage, while SOX2 protein firstly could be detected in cell nuclei at 8-cell stage. These differences suggested that OCT4, NANOG, and SOX2 played different function in regulating development of goat preimplantation embryos. These results may provide a novel insight to goat embryo development and be useful for goat ESCs isolation.

Key words: goat , OCT4 , NANOG , SOX2 , embryo , expression pattern