Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)

doi:10.1016/S2095-3119(13)60361-5

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (4): 722-732.DOI: 10.1016/S2095-3119(13)60361-5

Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)

WANG Xin-min; REN Xian; YIN Gui-xiang; WANG Ke; LI Jia-rui; DU Li-pu; XU Hui-jun ;

1.National Key Facility of Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.College of Life and Engineering Sciences, Beifang University of Nationalities, Yinchuan 750021, P.R.China
3.Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA

收稿日期:2013-01-29 出版日期:2014-04-01 发布日期:2014-04-16
通讯作者: Correspondence YE Xing-guo, Tel/Fax: +86-10-82109765, E-mail: yexingguo@caas.cn
基金资助:
This research was financially supported in part by the National Natural Science Foundation of China (30971776) and the Transgenic Major Projects, Ministry of Agriculture of China (2011ZX08010-004).

Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)

WANG Xin-min; REN Xian; YIN Gui-xiang; WANG Ke; LI Jia-rui; DU Li-pu; XU Hui-jun ;

1.National Key Facility of Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.College of Life and Engineering Sciences, Beifang University of Nationalities, Yinchuan 750021, P.R.China
3.Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA

Received:2013-01-29 Online:2014-04-01 Published:2014-04-16
Contact: Correspondence YE Xing-guo, Tel/Fax: +86-10-82109765, E-mail: yexingguo@caas.cn
Supported by:
This research was financially supported in part by the National Natural Science Foundation of China (30971776) and the Transgenic Major Projects, Ministry of Agriculture of China (2011ZX08010-004).

摘要/Abstract

摘要： The immature embryos (IEs) of wheat are the most widely used tissues for in vitro culture and genetic transformation due to its high regeneration competency. However, this explant can only be maintained in 4°C daily cooler for a short period time for its use in plant tissue culture or transformation experiments. This study aimed to investigate the effects of environmental temperature, cryopreservation storage temperature, and heat shock culture (HSC) temperature on the regeneration frequency of wheat IEs. Results indicated that environmental temperature significantly affected the induction of embryonic calli. The optimum total accumulated temperature (TAT) during the time of anthesis and sampling for regeneration of these tissues was around 280°C for spring wheat type cv. CB037 and approximately 300°C for winter wheat type cv. Kenong 199. Regeneration ability obviously declined when the highest environmental temperature was over 35°C for 1 d or a high temperature between 30 and 33°C lasted for 5 d during anthesis and sampling. This finding was verified by culturing the freshly isolated IEs under different temperatures from 29 to 37°C in different controlled growth incubators for 5 d; the IEs almost completely lost regeneration ability when the temperature rose to 37°C. Cryopreservation of -20°C caused the wheat samples lost ability of producing callus or embryonic callus in a few days, and cryopreservation of -10°C more than 10 d made the regeneration potential of the tissues dramatically declined. Comparatively, the temperature that best maintained high regeneration ability was -5°C, at which the materials can be maintained for around 1 mon. In addition, the preservation of the immature samples at -5 or -10°C inhibited the direct germination of the IEs, avoiding the embryo axis removing process. Our results are useful for ensuring that field collection and cryopreservation of the wheat IEs are done correctly to enable tissue culture and genetic transformation.

关键词: wheat , immature embryos , environmental temperature , preservation , plant regeneration

Abstract: The immature embryos (IEs) of wheat are the most widely used tissues for in vitro culture and genetic transformation due to its high regeneration competency. However, this explant can only be maintained in 4°C daily cooler for a short period time for its use in plant tissue culture or transformation experiments. This study aimed to investigate the effects of environmental temperature, cryopreservation storage temperature, and heat shock culture (HSC) temperature on the regeneration frequency of wheat IEs. Results indicated that environmental temperature significantly affected the induction of embryonic calli. The optimum total accumulated temperature (TAT) during the time of anthesis and sampling for regeneration of these tissues was around 280°C for spring wheat type cv. CB037 and approximately 300°C for winter wheat type cv. Kenong 199. Regeneration ability obviously declined when the highest environmental temperature was over 35°C for 1 d or a high temperature between 30 and 33°C lasted for 5 d during anthesis and sampling. This finding was verified by culturing the freshly isolated IEs under different temperatures from 29 to 37°C in different controlled growth incubators for 5 d; the IEs almost completely lost regeneration ability when the temperature rose to 37°C. Cryopreservation of -20°C caused the wheat samples lost ability of producing callus or embryonic callus in a few days, and cryopreservation of -10°C more than 10 d made the regeneration potential of the tissues dramatically declined. Comparatively, the temperature that best maintained high regeneration ability was -5°C, at which the materials can be maintained for around 1 mon. In addition, the preservation of the immature samples at -5 or -10°C inhibited the direct germination of the IEs, avoiding the embryo axis removing process. Our results are useful for ensuring that field collection and cryopreservation of the wheat IEs are done correctly to enable tissue culture and genetic transformation.

Key words: wheat , immature embryos , environmental temperature , preservation , plant regeneration

WANG Xin-min; REN Xian; YIN Gui-xiang; WANG Ke; LI Jia-rui; DU Li-pu; XU Hui-jun. Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)[J]. Journal of Integrative Agriculture, 2014, 13(4): 722-732.

WANG Xin-min; REN Xian; YIN Gui-xiang; WANG Ke; LI Jia-rui; DU Li-pu; XU Hui-jun ;. Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)[J]. Journal of Integrative Agriculture, 2014, 13(4): 722-732.

参考文献

[1]von Aderkas P, Kong L, Hawkins B, Rohr R. 2007. Effects of nonfreezing low temperatures on quality and cold tolerance of mature somatic embryos of interior spruce (Picea glauca (Moench) Voss×P. engelmannii Parry ex. Engelm.). Propagation of Ornamental Plants, 7, 112-121

[2]Aslam J, Mujib A, Sharma M P. 2011. Influence of freezing and non-freezing temperature on somatic embryogenesis and vinblastine production in Catharanthus roseus (L.) G. Don. Acta Physiologiae Plantarum, 33, 473-480

[3]Carman J G. 1988. Improved somatic embryogenesis in wheat by partial simulation of the in-ovulo oxygen, growth-regulator and desiccation environments. Planta, 175, 417-424

[4]Carman J G, Jefferson N E, Campbell W F. 1987. Induction of embryogenic Triticum aestivum L. calli. I. Quantification of genotype and culture medium effects. Plant Cell, Tissue and Organ Culture, 10, 101-113

[5]Carman J G, Jefferson N E, Campbell W F. 1988. Induction of embryogenic Triticum aestivum L. calli. II. Quantification of organic addenda and other culture variable effects. Plant Cell, Tissue and Organ Culture, 12, 97-110

[6]Caswell K L, Leung N L, Chibbar R N. 2000. An efficient method for in vitro regeneration from immature inflorescence explants of Canadian wheat cultivars. Plant Cell, Tissue and Organ Culture, 60, 69-73

[7]Chauhan H, Khurana P. 2011. Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics. Plant Biotechnology Journal, 9, 408-417

[8]Cheng M, Fry J E, Pang S, Zhou H, Hironaka C M, Duncan D R, Conner T W, Wan Y. 1997. Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiology, 115, 971-980

[9]Gémes-Juhász A, Balogh P, Ferenczy A, Kristóf Z. 2002. Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber (Cucumis sativus L.). Plant Cell Reports, 21, 105-111

[10]Greer M S, Kovalchuk I, Eudes F. 2009. Ammonium nitrate improves direct somatic embryogenesis and biolistic transformation of Triticum aestivum. New Biotechnology, 26, 44-52

[11]He D G, Yang Y M, Scott K J. 1988. A comparison of scutellum callus and epiblast callus induction in wheat: The effect of genotype, embryo age and medium. Plant Science, 57, 225-233

[12]He D G, Yang Y M, Scott K J. 1989. The effect of macroelements in the induction of embryogenic callus from immature embryos of wheat (Triticum aestivum L.). Plant Science, 64, 251-258

[13]He G Y, Lazzeri P A. 2001. Improvement of somatic embryogenesis and plant regeneration from durum wheat (Triticum turgidum var. durum Desf.) scutellum and inflorescence cultures. Euphytica, 119, 369-376

[14]Hess J R, Carman J G. 1998. Embryogenic competence of immature wheat embryos: genotype, donor plant environment and endogenous hormone levels. Crop Science, 38, 249-253

[15]Hou B, Yu H, Teng S. 1997. Effects of low temperature on induction and differentiation of wheat calli. Plant Cell, Tissue and Organ Culture, 49, 35-38

[16]Husaini A M, Abdin M. 2007. Interactive effect of light, temperature and TDZ on the regeneration potential of leaf discs of Fragaria ananassa Duch. In vitro Cellular & Developmental Biology-Plant, 43, 576-584

[17]Ilahi I, Ghauri E. 1994. Regeneration in cultures of Papaver bracteatum as influenced by growth hormones and temperature. Plant Cell, Tissue and Organ Culture, 38, 81-83

[18]Jones H D, Doherty A, Wu H. 2005. Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat. Plant Methods, 1, 5. Kamada H, Tachikawa Y, Saitou T, Harada H. 1994. Heat stress induction of carrot somatic embryogenesis. Plant Tissue Culture Letters, 11, 229-232

[19]Karami O, Saidi A. 2010. The molecular basis for stress-induced acquisition of somatic embryogenesis. Molecular Biology Reports, 37, 2493-2507

[20]Kong L S, von Aderkas P. 2011. A novel method of cryopreservation without a cryoprotectant for immature somatic embryos of conifer. Plant Cell, Tissue and Organ Culture, 106, 115-125

[21]Li B, Caswell K, Leung N, Chibbar R N. 2003. Wheat (Triticum aestivum L.) somatic embryogenesis from isolated scutellum: Days post anthesis, days of spike storage, and sucrose concentration affect efficiency. In Vitro Cellular & Developmental Biology-Plant, 39, 20-23

[22]Li J R, Ye X G, An B Y, Du L P, Xu H J. 2012. Genetic transformation of wheat: current status and future prospects. Plant Biotechnology Reports, 6, 183-193

[23]Libik M, Konieczny R, Pater B, Slesak I, Miszalski Z. 2005. Differences in the activities of some antioxidant enzymes and in H2O2 content during rhizogenesis and somatic embryogenesis in callus cultures of the ice plant. Plant Cell Reports, 23, 834-841

[24]Lin H C, Morcillo F, Dussert S, Tranchant-Dubreuil C, Tregear J, Tranbarger T. 2009. Transcriptome analysis during somatic embryogenesis of the tropical monocot Elaeis guineensis: evidence for conserved gene functions in early development. Plant Molecular Biology, 70, 173-192

[25]Liu W, Zheng M Y, Polle E A, Konzak C F. 2002. Highly efficient doubled-haploid production in wheat (Triticum aestivum L.) via induced microspore embryogenesis. Crop Science, 42, 686-692

[26]Mathur G, Alkutkar V, Nadgauda R. 2003. Cryopreservation of embryogenic culture of Pinus roxburghii. Biologia Plantarum, 46, 205-210

[27]Moriguchi T, Kozaki I, Yamaki S, Sanada T. 1990. Low temperature storage of pear shoots in vitro. Bulletin of the Fruit Tree Research Station, 17, 11-18

[28]Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497

[29]Nehra N S, Chibbar R N, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M, Kartha K K. 1994. Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. The Plant Journal, 5, 285-297

[30]Ogawa T, Kawahigashi H, Toki S, Handa H. 2008. Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker. Plant Cell Reports, 27, 1325-1331

[31]Ou Y J, Hu H, Zhuang J J, Zeng J Z. 1973. Production of wheat haploid plants from the pollens and observation of their offspring. Science in China, 1, 72-82

[32]Ozias-Akins P, Vasil I K. 1982. Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat): Evidence for somatic embryogenesis. Protoplasma, 110, 95-105

[33]Özgen M, Tüuret M, Altionk S, Sancak C. 1998. Efficient callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L.) genotypes. Plant Cell Reports, 18, 331-335

[34]Pastori G M, Wilkinson M D, Steele S H, Sparks C A, Jones H D, Parry M A J. 2001. Age-dependent transformation frequency in elite wheat varieties. Journal of Experimental Botany, 52, 857-863

[35]Rasco-Gaunt S, Riley A, Cannell M, Barcelo P, Lazzeri P A. 2001. Procedures allowing the transformation of a range of European elite wheat (Triticum aestivum L.) varieties via particle bombardment. Journal of Experimental Botany, 52, 865-874

[36]Sharma V K, Hansch R, Mendel R R, Schulze J. 2005. Influence of picloram and thidiazuron on high frequency plant regeneration in elite cultivars of wheat with long-term retention of morphogenecity using meristematic shoot segments. Plant Breeding, 124, 242-246

[37]She M Y, Yin G X, Li J R, Li X, Du L P, Ma W J, Ye X G. 2013. Efficient regeneration potential is closely related to auxin exposure time and catalase metabolism during the somatic embryogenesis of immature embryos in Triticum aestivum L. Molecular Biotechnology, 54, 451-460

[38]Shrawat A K, Lörz H. 2006. Agrobacterium-mediated transformation of cereals: A promising approach crossing barriers. Plant Biotechnology Journal, 4, 575-603

[39]Szechyńska-Hebda M, Skrzypek E, D?browska G, Biesaga-Ko?cielniak J, Filek M, W?dzony M. 2007. The role of oxidative stress induced by growth regulators in the regeneration process of wheat. Acta Physioogiael Plantarum, 29, 327-337

[40]Tao L L, Yin G X, Du L P, Shi Z Y, She M Y, Xu H J, Ye X G. 2011. Improvement of plant regeneration from immature embryos of wheat infected by Agrobacterium tumefaciens. Agricultural Sciences in China, 10, 317-326

[41]Wang B, Zhang Z, Yin Z, Feng C, Wang Q. 2012. Novel and potential application of cryopreservation to plant genetic transformation. Biotechnology Advances, 30, 604-612

[42]Wang Y L, Xu M X, Wang D W, Ye X G. 2009. Agrobacterium-mediated wheat transformation based on the mature embryo culture. Cereal Research Communications, 37, 1-12

[43]Wise R R, Olson A J, Schrader S M, Sharkey T D. 2004. Electron transport is the functional limitation of photosynthesis in field-grown pima cotton plants at high temperature. Plant, Cell and Environment, 27, 717-724

[44]Yin G X, Wang Y L, She M Y, Du L P, Xu H J, Ma J X, Ye X G. 2011. Establishment of a highly efficient regeneration system for the mature embryo culture of wheat. Agricultural Sciences in China, 10, 9-17

Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)

Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)

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