Effect of PMSG/hCG Superovulation on Mouse Embryonic Development

doi:10.1016/S2095-3119(13)60325-1

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (6): 1066-1072.DOI: 10.1016/S2095-3119(13)60325-1

Effect of PMSG/hCG Superovulation on Mouse Embryonic Development

href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((WU Bao-jiang[Author]) AND 1[Journal]) AND year[Order])" target="_blank">WU Bao-jiang, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((XUE Hong-yan[Author]) AND 1[Journal]) AND year[Order])" target="_blank">XUE Hong-yan, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((CHEN Li-ping[Author]) AND 1[Journal]) AND year[Order])" target="_blank">CHEN Li-ping, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((DAI Yan-feng[Author]) AND 1[Journal]) AND year[Order])" target="_blank">DAI Yan-feng, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((GUO Ji-tong[Author]) AND 1[Journal]) AND year[Order])" target="_blank">GUO Ji-tong, LI Xi-he

1 Inner Mongolia Saikexing Reproductive Biotechnology Co., Ltd., Helingeer 011517, P.R.China
2 Research Center for Animal Genetic Resources of Mongolia Plateau, Inner Mongolia University, Hohhot 010021, P.R.China

收稿日期:2012-01-20 出版日期:2013-06-01 发布日期:2013-06-09
通讯作者: Correspondence LI Xi-he, Tel: +86-471-7392175, Fax: +86-471-7392164, E-mail: lixihe@hotmail.com
作者简介:WU Bao-jiang, E-mail: wbjiang1983@163.com
基金资助:
This work is supported by the Natural Science Foundation of Inner Mongolia for the Major Special Projects, China (2010ZD05).

Effect of PMSG/hCG Superovulation on Mouse Embryonic Development

WU Bao-jiang, XUE Hong-yan, CHEN Li-ping, DAI Yan-feng, GUO Ji-tong, LI Xi-he

1 Inner Mongolia Saikexing Reproductive Biotechnology Co., Ltd., Helingeer 011517, P.R.China
2 Research Center for Animal Genetic Resources of Mongolia Plateau, Inner Mongolia University, Hohhot 010021, P.R.China

Received:2012-01-20 Online:2013-06-01 Published:2013-06-09
Contact: Correspondence LI Xi-he, Tel: +86-471-7392175, Fax: +86-471-7392164, E-mail: lixihe@hotmail.com
About author:WU Bao-jiang, E-mail: wbjiang1983@163.com
Supported by:
This work is supported by the Natural Science Foundation of Inner Mongolia for the Major Special Projects, China (2010ZD05).

摘要/Abstract

摘要： Kunming mouse strain is widely used in China, and the superovulation was administrated with 10 IU PMSG combined with 10 IU hCG. In this study, the effects of the exogenous gonadotropins on superovulation of Kunming mice and embryo quality derived from the superovulated mice were assessed. Female mice at 6-8-wk old were superovulated with 0, 5, 7.5 and 10 IU PMSG/hCG and mated with male mice. The embryos were retrieved at 2.5 d post coitum. No statistic difference was observed for the number of 2-cell embryos collected per mouse between control and 5 IU PMSG/hCG treatment group, but the number significantly increased for 7.5 and 10 IU PMSG/hCG treatment group (P<0.05). The average number of 4- cell and 8-cell embryos collected from each mouse significantly differed between control and 5, 7.5, 10 IU PMSG/hCG treatment groups (P<0.05). When 8-cell embryos derived from mice administrated with 0, 5, 7.5 and 10 IU PMSG/hCG were cultured in KSOM, the blastocyst development rates were 88.1, 94.7, 96.1 and 94.3%, respectively, which were similar to control (P>0.05). This indicated that exogenous gonadotropins have no effects on development of Kunming mouse embryos. The quality of blastocyst was assessed by labelling with Hoechst and propidium iodide for inner cell mass and trophectoderm cells, the result showed that ICM/TE ratio significantly decreased for 10 IU PMSG/hCG treatment group compared with control, 5 and 7.5 IU PMSG/hCG treatment group (P<0.05). This suggested that the embryo quality of Kunming mouse has been affected by high dose of gonadotropins.

关键词: PMSG , mouse , 8-cell embryos , in vitro culture , blastocysts

Abstract: Kunming mouse strain is widely used in China, and the superovulation was administrated with 10 IU PMSG combined with 10 IU hCG. In this study, the effects of the exogenous gonadotropins on superovulation of Kunming mice and embryo quality derived from the superovulated mice were assessed. Female mice at 6-8-wk old were superovulated with 0, 5, 7.5 and 10 IU PMSG/hCG and mated with male mice. The embryos were retrieved at 2.5 d post coitum. No statistic difference was observed for the number of 2-cell embryos collected per mouse between control and 5 IU PMSG/hCG treatment group, but the number significantly increased for 7.5 and 10 IU PMSG/hCG treatment group (P<0.05). The average number of 4- cell and 8-cell embryos collected from each mouse significantly differed between control and 5, 7.5, 10 IU PMSG/hCG treatment groups (P<0.05). When 8-cell embryos derived from mice administrated with 0, 5, 7.5 and 10 IU PMSG/hCG were cultured in KSOM, the blastocyst development rates were 88.1, 94.7, 96.1 and 94.3%, respectively, which were similar to control (P>0.05). This indicated that exogenous gonadotropins have no effects on development of Kunming mouse embryos. The quality of blastocyst was assessed by labelling with Hoechst and propidium iodide for inner cell mass and trophectoderm cells, the result showed that ICM/TE ratio significantly decreased for 10 IU PMSG/hCG treatment group compared with control, 5 and 7.5 IU PMSG/hCG treatment group (P<0.05). This suggested that the embryo quality of Kunming mouse has been affected by high dose of gonadotropins.

Key words: PMSG , mouse , 8-cell embryos , in vitro culture , blastocysts

WU Bao-jiang, XUE Hong-yan, CHEN Li-ping, DAI Yan-feng, GUO Ji-tong, LI Xi-he. Effect of PMSG/hCG Superovulation on Mouse Embryonic Development[J]. Journal of Integrative Agriculture, 2013, 12(6): 1066-1072.

WU Bao-jiang, XUE Hong-yan, CHEN Li-ping, DAI Yan-feng, GUO Ji-tong, , LI Xi-he. Effect of PMSG/hCG Superovulation on Mouse Embryonic Development[J]. Journal of Integrative Agriculture, 2013, 12(6): 1066-1072.

参考文献

[1]Allen J, McLaren A. 1971. Cleavage rate of mouse eggsfrom induced and spontaneous ovulation. Journal ofReproduction and Fertility, 27, 137-140

[2]van der Auwera I, D’Hooghe T. 2001. Superovulation offemale mice delays embryonic and fetal development.Human Reproduction, 16, 1237.

[3]van der Auwera I, Pijnenborg R, Koninckx P. 1999. Theinfluence of in-vitro culture versus stimulated anduntreated oviductal environment on mouse embryodevelopment and implantation. Human Reproduction,14, 2570.

[4]Edwards R G, Fowler R E. 1959. Fetal mortality in adult miceafter superovulation with gonadotrophins. Journal ofExperimental Zoology, 141, 299-322

[5]Elbling L, Colot M. 1985. Abnormal development andtransport and increased sister-chromatid exchange inpreimplantation embryos following superovulation inmice. Mutation Research/Environmental Mutagenesisand Related Subjects, 147, 189-195

[6]Erbach G T, Lawitts J A, Papaioannou V E, Biggers J D.1994. Differential growth of the mouse preimplantationembryo in chemically defined media. Biology ofReproduction, 50, 1027-1033

[7]Ertzeid G, Storeng R. 1992. Adverse effects ofgonadotrophin treatment on pre- and postimplantationdevelopment in mice. Journal of Reproduction andFertility, 96, 649.

[8]Ertzeid G, Storeng R. 2001. The impact of ovarian stimulationon implantation and fetal development in mice. HumanReproduction, 16, 221.

[9]Fortier A L, Lopes F L, Darricarrere N, Martel J, Trasler J M.2008. Superovulation alters the expression of imprintedgenes in the midgestation mouse placenta. HumanMolecular Genetics, 17, 1653.

[10]Fowler R E, Edwards R G. 1957. Induction of superovulationand pregnancy in mature mice by gonadotrophins.Journal of Endocrinology, 15, 374-384

[11]Gates A H. 1956. Viability and developmental capacity ofeggs from immature mice treated with gonadotrophins.Nature, 177, 754-755

[12]Jones E C, Krohn P L. 1961. The relationships between age,numbers of ocytes and fertility in virgin and multiparousmice. Journal of Endocrinology, 21, 469-495

[13]Lee S T, Kim T M, Cho M Y, Moon S Y, Han J Y, Lim J M.2005. Development of a hamster superovulation programand adverse effects of gonadotropins on microfilamentformation during oocyte development. Fertility andSterility, 83, 1264-1274

[14]Lei L, Liu Z H, Zhu Z Y, Kou Z H, Wu Y Q, Xu Y, Wen D C,Bi C M, Xia G L, Chen D Y. 2003. Effects of differentnuclear recipients on developmental potential of mousesomatic nuclear transfer embryos. Chinese ScienceBulletin, 48, 469-471

[15]Luo C, Zuñiga J, Edison E, Palla S, Dong W, Parker-Thornburg J. 2011. Superovulation strategies for 6commonly used mouse strains. Journal of the AmericanAssociation for Laboratory Animal Science, 50, 471-478

[16]Market-Velker B A, Zhang L, Magri L S, Bonvissuto A C,Mann M R W. 2010. Dual effects of superovulation:loss of maternal and paternal imprinted methylation ina dose-dependent manner. Human Molecular Genetics,19, 36.

[17]McLaren A, Michie D. 1959. Superregnancy in the mouse.Journal of Experimental Biology, 36, 281-284

[18]Men H S, Chen J C, Ji W Z, Shang E Y, Yang S C, Zou R J.1997. Cryopreservation of kunming mouse oocytesusing slow cooling, ultrarapid cooling and vitrificationprotocols. Theriogenology, 47, 1423-1431

[19]Nagy A. 2003. Manipulating the Mouse Embryo: aLaboratory Manual. Cold Spring Harbor LaboratoryPress, Cold Spring Harbor, NY.Takaoka K, Hamada H. 2012. Cell fate decisions and axisdetermination in the early mouse embryo. Development,139, 3-14

[20]Theiler K. 1989. The House Mouse: Atlas of EmbryonicDevelopment. Springer-Verlag, New York.Thouas G A, Korfiatis N A, French A J, Jones G M, Trounson A O. 2001. Simplified technique for differential stainingof inner cell mass and trophectoderm cells of mouseand bovine blastocysts. Reproductive BioMedicineOnline, 3, 25-29

[21]Vergara G J, Irwin M H, Moffatt R J, Pinkert C A. 1997. Invitro fertilization in mice: strain differences in responseto superovulation protocols and effect of cumulus cellremoval. Theriogenology, 47, 1245-1252

[22]Walton E A, Armstrong D. 1983. Oocyte normality aftersuperovulation in immature rats. Journal ofReproduction and Fertility, 67, 309.Walton E A, Evans G, Armstrong D T. 1983. Ovulationresponse and fertilization failure in immature ratsinduced to superovulate. Journal of Reproduction andFertility, 67, 91-96

[23]Walton E A, Huntley S, Kennedy T G, Armstrong D T.1982. Possible causes of implantation failure insuperovulated immature rats. Biology of Reproduction,27, 847-852

[24]Wilson E D, Zarrow M X. 1962. Comparison ofsuperovulation in the immature mouse and rat. Journalof Reproduction and Fertility, 3, 148-158

[25]Yun Y W, Yu F H, Yuen B H, Moon Y S. 1989. Effects of asuperovulatory dose of pregnant mare serumgonadotropin on follicular steroid contents and oocytematuration in rats. Gamete Research, 23, 289-298

[26]Yun Y W, Yuen B H, Moon Y S. 1987. Effects ofsuperovulatory doses of pregnant mare serumgonadotropin on oocyte quality and ovulatory andsteroid hormone responses in rats. Gamete Research,16, 109-120

Effect of PMSG/hCG Superovulation on Mouse Embryonic Development

Effect of PMSG/hCG Superovulation on Mouse Embryonic Development

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

[1]	CHU Zhi-li, LIU Chao, BAI Yao-fu, ZHU Hai-jing, HU Yue , HUA Jin-lian. A Three-Dimensional (3D) Environment to Maintain the Integrity of Mouse Testicular Can Cause the Occurrence of Meiosis[J]. Journal of Integrative Agriculture, 2013, 12(8): 1481-1488.
[2]	SHI Ya-ran, WANG Zhan-he, CAO Yong-chun, LU Yan, TIAN Jin-ling, ZhANG Chao, JIA Zi-ye, CHEN Wu. Relationships Between Icariin and Anti-Apoptotic miRNA-21 in Mouse Blastocyst Development In vitro[J]. Journal of Integrative Agriculture, 2013, 12(4): 663-669.
[3]	YU Meng, WANG Long, HU Yue, LIAN Zhi-min , HUA Jin-lian. ALK Family Inhibitor A83-01 Promotes the Proliferation of Mouse Male Germline Stem Cells (mGSCs) Under Serum- and Feeder-Free Conditions[J]. Journal of Integrative Agriculture, 2013, 12(10): 1839-1846.
[4]	HU Ge, XUE Jiu-zhou, LIU Jing, ZHANG Tao, DONG Hong, DUAN Hui-qin, YANG Zuo-jun, RENXiao-ming , MU Xiang. Baicalin Induces IFN-α/β and IFN-γ Expressions in Cultured Mouse Pulmonary Microvascular Endothelial Cells[J]. Journal of Integrative Agriculture, 2012, 12(4): 646-654.
[5]	ZHAO Xue-ming, WANG Dong, QIN Tong, LIU Yan, ZHU Hua-bin. Controlled Freezing and Open-Pulled Straw (OPS) Vitrification of In vitro Produced Bovine Blastocysts Following Analysis of ATP Content and Reactive Oxygen Species (ROS) Level[J]. Journal of Integrative Agriculture, 2012, 12(3): 446-455.