AcMYB12和AcMYB29通过转录调控促进洋葱黄酮醇的生物合成

doi:10.1016/j.jia.2025.06.023

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (3): 1035-1050.DOI: 10.1016/j.jia.2025.06.023

AcMYB12和AcMYB29通过转录调控促进洋葱黄酮醇的生物合成

收稿日期:2024-06-18 修回日期:2025-06-24 接受日期:2024-11-11 出版日期:2026-03-20 发布日期:2026-02-06

AcMYB12 and AcMYB29 promote flavonol biosynthesis through transcriptional regulation in onion (Allium cepa L.)

Qingwei Jia^{1, 2}, Shuting Gai^{1, 2}, Yiren Wang^{1, 2}, Zhihui Zhang^{1, 2}, Xiong Wu³, Wenhui Wu^{1, 2}, Yumeng Pang^{1, 2}, Xiaonan Zhang^{1, 2}, Lei Qin^{1, 2#}, Yong Wang^{1, 2#}

¹College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China
² Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region) of Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, China
³Henan Kanglong Hi-tech Seed Co., Ltd., Jiyuan 459000, China

Received:2024-06-18 Revised:2025-06-24 Accepted:2024-11-11 Online:2026-03-20 Published:2026-02-06
About author:Qingwei Jia, E-mail: 465872232@qq.com; #Correspondence Lei Qin, Tel: +86-451-55191261, E-mail: qinlei@neau.edu.cn; Yong Wang, Tel: +86-451-55190243, E-mail: yongwang@neau.edu.cn
Supported by:
This work was supported by the Key R&D Projects in Heilongjiang Province, China (GA21B012); the Collaborative Innovation Achievement Project of University in Heilongjiang Province, China (LJGXCG2022-040).

摘要/Abstract

摘要：

黄酮醇具有很高的药用价值，不但在植物抗逆性具有重要作用，而且也是洋葱营养价值的关键组成部分，尤其是在可食用部分。虽然黄酮醇的生物合成途径已经得到了很好的研究，但在洋葱中的调控作用尚不完全清楚。本研究通过分析“SA1”不同发育阶段的转录组学和代谢组学数据，筛选到了黄酮醇生物合成和调控基因。其中，鉴定了两个R2R3-MYB转录因子AcMYB12和AcMYB29，是洋葱黄酮醇生物合成的正调控因子。转录激活实验表明，它们都能激活黄酮醇生物合成途径基因AcCHS、AcF3’H和AcFLS的转录，而酵母单杂交实验证实它们直接结合这些基因的启动子。过表达洋葱愈伤组织和拟南芥中黄酮醇途径基因的表达量和黄酮醇含量显著高于对照，进一步证实AcMYB29和AcMYB12在黄酮醇调控中的作用。瞬时沉默试验显示两者之间存在部分功能冗余。有趣的是，他们的调节能力也存在显著差异。AcMYB12主要调控黄酮醇积累，而AcMYB29主要调控槲皮素的合成。进一步研究了它们之间的差异调控的分子机制，结果表明，这些差异可能是由于黄酮醇生物合成途径基因启动子中顺式元件的多样性以及转录因子和顺式元件之间结合活性的差异。

Abstract:

Flavonols possess significant medical value and are essential for plant stress resistance. These compounds constitute primary components of the nutritional value in onions, particularly in edible portions. While the flavonol biosynthetic pathway has been extensively studied, its regulatory mechanisms in onions remain incompletely understood. This investigation identified flavonol biosynthesis and regulatory genes through analysis of transcriptome and metabolomics data from different developmental stages of ‘SA1’. Two R2R3-MYB transcription factors, AcMYB12 and AcMYB29, were identified as positive regulators of onion flavonol biosynthesis. Transcriptional activation assays demonstrated that both could activate AcCHS, AcF3´H, and AcFLS. Yeast one-hybrid assays confirmed their direct binding to these gene promoters. The expression levels of flavonol pathway genes and flavonol contents in AcMYB12/AcMYB29-overexpressing onion calli and Arabidopsis plants were significantly higher than those in the control group. Transient silencing assays revealed partial functional redundancy between these two transcription factors. Notably, their regulatory capabilities exhibited significant differences. AcMYB12 predominantly regulates flavonol accumulation, while AcMYB29 specifically influences quercetin. Further investigation of the molecular mechanisms underlying differential regulation indicated variations in cis-elements within flavonol pathway gene promoters and differences in binding activity between transcription factors and cis-elements.

Key words: onion , flavonols , AcMYB12 , AcMYB29 , cis-element , flavonol pathway genes

Qingwei Jia, Shuting Gai, Yiren Wang, Zhihui Zhang, Xiong Wu, Wenhui Wu, Yumeng Pang, Xiaonan Zhang, Lei Qin, Yong Wang. AcMYB12和AcMYB29通过转录调控促进洋葱黄酮醇的生物合成[J]. Journal of Integrative Agriculture, 2026, 25(3): 1035-1050.

Qingwei Jia, Shuting Gai, Yiren Wang, Zhihui Zhang, Xiong Wu, Wenhui Wu, Yumeng Pang, Xiaonan Zhang, Lei Qin, Yong Wang. AcMYB12 and AcMYB29 promote flavonol biosynthesis through transcriptional regulation in onion (Allium cepa L.)[J]. Journal of Integrative Agriculture, 2026, 25(3): 1035-1050.

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AcMYB12和AcMYB29通过转录调控促进洋葱黄酮醇的生物合成

AcMYB12 and AcMYB29 promote flavonol biosynthesis through transcriptional regulation in onion (Allium cepa L.)

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