StCOMT1通过调节香豆素相关物质积累和ROS系统增强马铃薯对拟枝孢镰刀菌的抗性

doi:10.1016/j.jia.2025.11.021

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (3): 1020-1035.DOI: 10.1016/j.jia.2025.11.021

StCOMT1通过调节香豆素相关物质积累和ROS系统增强马铃薯对拟枝孢镰刀菌的抗性

收稿日期:2024-11-11 修回日期:2025-11-15 接受日期:2025-05-19 出版日期:2026-03-20 发布日期:2026-02-06

StCOMT1 enhances potato resistance to Fusarium sporotrichioides by regulating coumarin-related substance accumulation and the ROS system

Jiaqi Wang^{1, 2*}, Biao Zhao^{1, 2*}, Dan Liu¹, Fumeng He¹, Chong Du¹, Yunzhu Che¹, Zengli Zhang¹, Xu Feng¹, Xue Wang³ ,Yingnan Wang^1#, Fenglan Li^{1, 2, 3#}

¹ College of Life Sciences, Northeast Agricultural University, Harbin 150030, China
²Heilongjiang High Quality Agricultural Functional Microbial Agent Engineering and Technology Research Centre, Harbin 150030, China
³ Heilongjiang Green Food Science Research Institute, Harbin 150028, China

Received:2024-11-11 Revised:2025-11-15 Accepted:2025-05-19 Online:2026-03-20 Published:2026-02-06
About author:Jiaqi Wang, E-mail: wjq13941677600@163.com; Biao Zhao, E-mail: zhaob35@outlook.com; #Correspondence Yingnan Wang, E-mail: yingnan1027@foxmail.com; Fenglan Li, E-mail: lifenglan@neau.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (U22A20443); the Heilongjiang Provincial Research Institutes Scientific Research Operating Expenses Project, China (CZKYF2023-1-B020); the Key Research and Development Plan Project of Heilongjiang Province, China (GA23B015) and the Young Scientists Fund of the National Natural Science Foundation of China (32201717).

摘要/Abstract

摘要：

咖啡酸-O-甲基转移酶（COMT）是苯丙烷代谢途径中的关键酶，在木质素与香豆素代谢途径中都发挥着重要作用。COMT在植物抗病方面的功能已在多个物种得到证实。本研究在全基因组水平对马铃薯COMT基因家族进行了鉴定，并通过系统发育分析结合前期代谢差异、加权基因共表达网络分析（WGCNA），确定了StCOMT1是脱氧雪腐镰刀菌烯醇（DON）诱导下增强马铃薯抗病性增强的候选基因。为了更好地了解StCOMT1基因的功能，我们进行了StCOMT1的异源表达与过表达。StCOMT1定位于叶绿体中，异源表达发现它可以催化底物的甲基化生成阿魏酸与褪黑素。生理指标显示相较于野生型马铃薯植株，StCOMT1过表达植株受到拟枝孢镰刀菌感染后病斑面积更小，活性氧（ROS）水平降低。基于高效液相色谱（HPLC）表达谱与RT-qPCR数据的分析发现,接菌后，在StCOMT1过表达植株中香豆素相关化合物与香豆素相关基因呈现器官差异积累与表达。结果表明StCOMT1过表达马铃薯通过增强活性氧清除，促进香豆素相关化合物器官特异性积累，从而增强对拟枝孢镰刀菌的抗性。

Abstract:

Caffeic acid-O-methyltransferase (COMT) is a crucial enzyme in the phenylpropanoid metabolic pathway, with significant roles in both the lignin and coumarin pathways. The function of COMT in plant disease resistance has been demonstrated in several species. Our research identified the potato COMT gene family on a genome-wide scale and StCOMT1 as a candidate gene for enhancing potato disease resistance under DON induction through phylogenetic analyses combined with previously identified metabolic differences and weighted gene co-expression network analysis (WGCNA) results. In order to better understand the function of StCOMT1, heterologous expression and overexpression assays were conducted. StCOMT1 is localized in chloroplasts and was found to catalyze the methylation of substrates to produce ferulic acid and melatonin in vitro. Physiological parameters showed that, compared with wild-type potato plants, StCOMT1-overexpressing plants infected with Fusarium sporotrichioides exhibited smaller lesion areas and lower reactive oxygen species (ROS) levels. High-performance liquid chromatography (HPLC) and RT-qPCR analyses revealed organ-specific accumulation of coumarin-related compounds and organ-specific expression of their corresponding genes in StCOMT1-overexpressing plants post-inoculation. The results indicate that StCOMT1 overexpression in potatoes enhanced resistance to F. sporotrichioides by enhancing reactive oxygen species clearance and promoting organ-specific accumulation of coumarin-related compounds.

Key words: potato , COMT gene , overexpression , disease resistance , ROS scavenging

Jiaqi Wang, Biao Zhao, Dan Liu, Fumeng He, Chong Du, Yunzhu Che, Zengli Zhang, Xu Feng, Xue Wang, Yingnan Wang, Fenglan Li. StCOMT1通过调节香豆素相关物质积累和ROS系统增强马铃薯对拟枝孢镰刀菌的抗性[J]. Journal of Integrative Agriculture, 2026, 25(3): 1020-1035.

Jiaqi Wang, Biao Zhao, Dan Liu, Fumeng He, Chong Du, Yunzhu Che, Zengli Zhang, Xu Feng, Xue Wang , Yingnan Wang, Fenglan Li. StCOMT1 enhances potato resistance to Fusarium sporotrichioides by regulating coumarin-related substance accumulation and the ROS system[J]. Journal of Integrative Agriculture, 2026, 25(3): 1020-1035.

参考文献

Ahammed G J, Li Z, Chen J, Dong Y, Qu K, Guo T, Wang F, Liu A, Chen S, Li X. 2024. React ive oxygen species signaling in melatonin-mediated plant stress response. Plant Physiology and Biochemistry, 207, 108398.

Beyer S F, Beesley A, Rohmann P F W, Schultheiss H, Conrath U, Langenbach C J G. 2019. The A rabidopsis non-host defence-associated coumarin scopoletin protects soybean from Asian soybean rust. The Plant Journal, 99, 397–413.

Bruce M A, Shoup Rupp J L. 2019. Agrob acterium-mediated transformation of Solanum tuberosum L., potato. Methods in Molecular Biology (Clifton, N.J.), 1864, 203–223.

Camire M E, Kubow S, Donnelly D J. 2009. Potatoes and human health. Critical Reviews in Food Science and Nutrition, 49, 823–840.

Cannon S B, Mitra A, Baumgarten A, Young N D, May G. 2004. The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana. BMC Plant Biology, 4, 10.

Chang J J, Guo Y L, Yan J Y, Zhang Z X, Yuan L, Wei C H, Zhang Y, Ma J X, Yang J Q, Zhang X, Li H. 2021. The r ole of watermelon caffeic acid O-methyltransferase (ClCOMT1) in melatonin biosynthesis and abiotic stress tolerance. Horticulture Research, 8, 210.

Chao J T, Kong Y Z, Wang Q, Sun Y H, Gong D P, Lv J, Liu G S. 2015. MapGene2Chrom, a tool to draw gene physical map based on Perl and SVG languages. Hereditas, 37, 91–97. (in Chinese)

Chen C J, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. 2020. TBtools: An integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13, 1194–1202.

Chen Q, Chen Y, Li X, Zhang L, Rengel Z. 2025. Phyto melatonin: Biosynthesis, signaling, and functions. Annual Review of Plant Biology, 76, 171–195.

Daly P, McClellan C, Maluk M, Oakey H, Lapierre C, Waugh R, Stephens J, Marshall D, Barakate A, Tsuji Y, Goeminne G, Vanholme R, Boerjan W, Ralph J, Halpin C. 2018. RNAi-suppression of barley caffeic acid O-methyltransferase modifies lignin despite redundancy in the gene family. Plant Biotechnology Journal, 17, 594–607.

Daudi A, O’Brien J A. 2012. Detection of hydrogen peroxide by DAB staining in Arabidopsis leaves. Bio-Protocol, 2, e263.

Doppler M, Kluger B, Bueschl C, Steiner B, Buerstmayr H, Lemmens M, Krska R, Adam G, Schuhmacher R. 2019. Stable i sotope-assisted plant metabolomics: Investigation of phenylalanine-related metabolic response in wheat upon treatment with the Fusarium virulence factor deoxynivalenol. Frontiers in Plant Science, 10, 1137.

Duan S F, Yu J C, Baldwin T C, Yuan Y, Xiang G S, Cui R, Zhao Y, Mo X C, Lu Y C, Liang Y L. 2024. Genome-wide identification of a MADS-box transcription factor family and their expression during floral development in Coptis teeta wall. BMC Plant Biology, 24, 1023.

Fan Y L, Zhang W N, Kang Y C, Shi M F, Yang X Y, Yu H F, Zhang R Y, Liu YH, Qin S H. 2021. Physiolo gical and dynamic transcriptome analysis of two potato varieties reveal response of lignin and MAPK signal to dry rot caused by Fusarium sulphureum. Scientia Horticulturae, 289, 110470.

Feng Y M, Qi P Y, Xiao W L, Zhang T H, Zhou X, Liu L W, Yang S. 2022. Fabrication of isopropanolamine-decorated coumarin derivatives as novel quorum sensing inhibitors to suppress plant bacterial disease. Journal of Agricultural and Food Chemistry, 70, 6037–6049.

Ferruz E, Atanasova-Pénichon V, Bonnin-Verdal M N, Marchegay G, Pinson-Gadais L, Ducos C, Lorán S, Ariño A, Barreau C, Richard-Forget F. 2016. Effects of phenolic acids on the growth and production of T-2 and HT-2 toxins by Fusarium langsethiae and F. sporotrichioides. Molecules (Basel, Switzerland), 21, 449.

Funnell-Harris D, Sattler S, O’Neill P, Gries T, Ge Z, Nersesian N. 2023. Effects of altering three steps of monolignol biosynthesis on sorghum responses to stalk pathogens and water deficit. Plant Disease, 107, 3984–3995.

Gao L Y, Wang P, Yan X, Li J M, Ma L, Hu M H, Ge X Y, Li F G, Hou Y X. 2024. Feruloyl-CoA 6´-hydroxylase-mediated scopoletin accumulation enhances cotton resistance to Verticillium dahliae. Plant Physiology, 196, 3007–3022.

He Y D, Zhang J, He Y W, Liu H H, Wang C, Guan G P, Zhao Y C, Tian Y, Zhong X H, Lu X Y. 2023. Two O-methyltransferases are responsible for multiple O-methylation steps in the biosynthesis of furanocoumarins from Angelica decursiva. Plant Physiology and Biochemistry, 204, 108142.

Hu Q, Min L, Yang X Y, Jin S X, Zhang L, Li Y Y, Ma Y Z, Qi X W, Li D Q, Liu H B, Lindsey K, Zhu L F, Zhang X L. 2018. Laccase GhLac1 modulates broad-spectrum biotic stress tolerance via manipulating phenylpropanoid pathway and jasmonic acid synthesis. Plant Physiology, 176, 1808–1823.

Jiang C, Song J Z, Zhang J Z, Yang Q. 2017. Identificat ion and characterization of the major antifungal substance against Fusarium sporotrichioides from Chaetomium globosum. World Journal of Microbiology & Biotechnology, 33, 1–9.

Jiang T, Hao T W, Chen W J, Li CL, Pang S Q, Fu C L, Cheng J, Zhang C B, Ghorbanpour M, Miao S. 2025. Reprogramme d plant metabolism during viral infections: mechanisms, pathways and implications. Molecular Plant Pathology, 26, e70066.

Kong J, Xie Y F, Yu H, Guo Y H, Cheng Y L, Qian H, Yao W R. 2021. Synergistic antifungal mechanism of thymol and salicylic acid on Fusarium solani. LWT-Food Science and Technology, 140, 110787.

Kundu P, Shinde S, Grover S, Sattler S, Louis J. 2025. Caffeic aci d O-methyltransferase-dependent flavonoid defenses promote sorghum resistance to fall armyworm infestation. Plant Physiology, 197, kiaf071.

Lacaze A, Joly D L. 2020. Structural specificity in plant–filamentous pathogen interactions. Molecular Plant Pathology, 21, 1513–1525.

Lam L, Lui A, Bartley L, Mikami B, Umezawa T, Lo C. 2024. Multifuncti onal 5-hydroxyconiferaldehyde O-methyltransferases (CAldOMTs) in plant metabolism. Journal of Experimental Botany, 75, 1671–1695.

Letunic I, Bork P. 2007. Interactive tree of life (iTOL): An online tool for phylogenetic tree display and annotation. Bioinformatics, 23, 127–128.

Li C, Wang P, Wei Z W, Liang D, Liu C H, Yin L H, Jia D F, Fu M Y, Ma F W. 2012. The mitigation effects of exogenous melatonin on salinity-induced stress in Malus hupehensis. Journal of Pineal Research, 53, 298–306.

Li L, Wang S C, Yang X F, Francis F, Qiu D W. 2019. Protein elicitor PeaT1 enhanced resistance against aphid (Sitobion avenae) in wheat. Pest Management Science, 76, 236–243.

Li W, Lu J X, Lu K, Yuan J L, Huang J H, Du H, Li J. 2016. Cloning and phylogenetic analysis of Brassica napus L. caffeic acid O-methyltransferase 1 gene family and its expression pattern under drought stress. PLoS ONE, 11, e0165975.

Li Z, Zhang Y, Ren J, Jia F L, Zeng H M, Li G Y, Yang X F. 2022. Ethylene-responsive factor ERF114 mediates fungal pathogen effector PevD1-induced disease resistance in Arabidopsis thaliana. Molecular Plant Pathology, 23, 819–831.

Liang S M, Xu S B, Qu D, Yang L M, Wang J G, Liu H L, Xin W, Zou D T, Zheng H L. 2022. Identification and functional analysis of the caffeic acid O-methyltransferase (COMT) gene family in rice (Oryza sativa L.). International Journal of Molecular Sciences, 23, 8491.

Liu H B, Wang Y R, Chang Q Z, Li Q B, Fang J H, Cao N, Tong X J, Jiang X M, Yu X H, Cheng Y. 2024. Combined metabolome and transcriptome reveal HmF6´H1 regulating simple coumarin accumulation against powdery mildew infection in Heracleum moellendorffii Hance. BMC Plant Biology, 24, 507.

Liu J X, Wang P Y, Wang Y F, Zhang Y J, Xu T Q, Zhang Y Q, Xi J, Hou L J, Li L, Zhang Z Q, Lin Y B. 2022. Negative effects of poly (butylene adipate-co-terephthalate) microplastics on Arabidopsis and its root-associated microbiome. Journal of Hazardous Materials, 437, 129294.

Liu Y S, Wang Y Z, Pei J B, Li Y D, Sun H Y. 2021. Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit. BMC Plant Biology, 21, 5.

Lu S W, Zhuge Y X, Hao T Y, Liu Z J, Zhang M W, Fang J G. 2022. Systematic analysis reveals O-methyltransferase gene family members involved in flavonoid biosynthesis in grape. Plant Physiology and Biochemistry, 173, 33–45.

Ma L, Jiang H, Ren Y Y, Yang J W, Han Y, Si H J, Prusky D, Bi Y, Wang Y. 2022. Overexpression of StCDPK23 promotes wound healing of potato tubers by regulating StRbohs. Plant Physiology and Biochemistry, 185, 279–289.

Ma Q H, Xu Y, Lin Z B, He P. 2002. Cloning of cDNA encoding COMT from wheat which is differentially expressed in lodging-sensitive and -resistant cultivars. Journal of Experimental Botany, 53, 2281–2282.

Morimura H, Ito M, Yoshida S, Koitabashi M, Tsushima S, Camagna M, Chiba S, Takemoto D, Kawakita K, Sato I. 2020. In vitro assessment of biocontrol effects on Fusarium head blight and deoxynivalenol (DON) accumulation by DON-degrading bacteria. Toxins, 12, 399.

Pasare S A, Ducreux L J M, Morris W L, Campbell R, Sharma S K, Roumeliotis E, Kohlen W, van der Krol S, Bramley P M, Roberts A G, Fraser P D, Taylor M A. 2013. The role of the potato (Solanum tuberosum) CCD8 gene in stolon and tuber development. The New Phytologist, 198, 1108–1120.

Quentin M, Allasia V, Pegard A, Allais F, Ducrot P H, Favery B, Levis C, Martinet S, Masur C, Ponchet M, Roby D, Schlaich N L, Jouanin L, Keller H. 2009. Imbalanced lignin biosynthesis promotes the sexual reproduction of homothallic oomycete pathogens. PLoS Pathogens, 5, e1000264.

Saluja M, Zhu F, Yu H, Walia H, Sattler S E. 2020. Loss of COMT activity reduces lateral root formation and alters the response to water limitation in sorghum brown midrib (bmr) 12 mutant. New Phytologist, 229, 2780–2794.

Seong E S, Yoo J H, Lee J G, Kim H Y, Hwang I S, Heo K, Kim J K, Lim J D, Sacks E J, Yu C Y. 2012. Antisense-overexpression of the MsCOMT gene induces changes in lignin and total phenol contents in transgenic tobacco plants. Molecular Biology Reports, 40, 1979–1986.

Smakowska E, Kong J, Busch W, Belkhadir Y. 2016. Organ-specific regulation of growth-defense tradeoffs by plants. Current Opinion in Plant Biology, 29, 129–137.

Stokstad E. 2019. The new potato. Science, 363, 574–577.

Stringlis I A, Yu K, Feussner K, de Jonge R, Van Bentum S, Van Verk M C, Berendsen R L, Bakker P A H M, Feussner I, Pieterse C M J. 2018. MYB72-dependent coumarin exudation shapes root microbiome assembly to promote plant health. Proceedings of the National Academy of Sciences of the United States of America, 115, E5213–E5222.

Tiwari R K, Lal M K, Kumar R, Sharma S, Sagar V, Kumar A, Singh B, Aggarwal R. 2023. Impact of Fusarium infection on potato quality, starch digestibility, in vitro glycemic response, and resistant starch content. Journal of Fungi, 9, 466.

Wang M X, Zhu X L, Wang K, Lu C G, Luo M Y, Shan T L, Zhang Z Y. 2018. A wheat caffeic acid 3-O-methyltransferase TaCOMT-3D positively contributes to both resistance to sharp eyespot disease and stem mechanical strength. Scientific Reports, 8, 6543.

Wang Q, Teng M K, Li X. 2018. Functional and structural characterization of a novel catechol-O-methyltransferase from Schizosaccharomyces pombe. IUBMB Life, 71, 330–339.

Wang Q, Veley K, Johnson J, Sumner J, van Erven G, Kabel M, Dhungana S, Berry J, Boyher A, Braun D, Vermerris W, Bart R. 2025. Three Xanthomonas cell wall degrading enzymes and sorghum Brown midrib 12 contribute to virulence and resistance in the bacterial leaf streak pathosystem. Molecular Plant–Microbe Interactions, 38, 400–410.

Wang W Y, Su S S, Wang S Z, Ye L D, Yu H W. 2019. Significantly improved catalytic efficiency of caffeic acid O-methyltransferase towards N-acetylserotonin by strengthening its interactions with the unnatural substrate’s terminal structure. Enzyme and Microbial Technology, 125, 1–5.

Wang Y P, Tang H B, DeBarry J D, Tan X, Li J P, Wang X Y, Lee T H, Jin H Z, Marler B, Guo H, Kissinger J C, Paterson A H. 2012. MCScanX: A toolkit for detection and evolutionary analysis of gene synteny and collinearity. Nucleic Acids Research, 40, e49.

van Wees S. 2008. Ph enotypic analysis of Arabidopsis mutants: Trypan blue stain for fungi, oomycetes, and dead plant cells. CSH Protocols, doi: 10.1101/pdb.prot4982.

Wei X H, Feng X, He F M, Xu Y Q, Li J Y, Xiao M M, Li L, Li C T, Yuan Q, Li F L. 2023. Efficacy of vomitoxin as elicitor for controlling soilborne diseases on potato plants. Journal of Fujian Agricultural Sciences, 38, 329–338. (in Chinese)

Xiao S H, Hu Q, Shen J L, Liu S M, Yang Z G, Chen K, Klosterman S J, Javornik B, Zhang X L, Zhu L F. 2021. GhMYB4 downregulates lignin biosynthesis and enhances cotton resistance to Verticillium dahliae. Plant Cell Reports, 40, 735–751.

Xue H L, Bi Y, Wei J M, Tang Y M, Zhao Y, Wang Y. 2013. New method for the simultaneous analysis of types A and B trichothecenes by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry in potato tubers inoculated with Fusarium sulphureum. Journal of Agricultural and Food Chemistry, 61, 9333–9338.

Xue H L, Bi Y, Zong Y Y, Alejandro C, Wang H J, Pu L M, Wang Y, Li Y C. 2017. Effects of elicitors on trichothecene accumulation and Tri genes expression in potato tubers inoculated with Fusarium sulphureum. European Journal of Plant Pathology, 148, 673–685.

Xue H L, Liu Q L, Yang Z M. 2023. Pathogenicity, mycotoxin production, and control of potato dry rot caused by Fusarium spp.: A Review. Journal of Fungi, 9, 843.

Ya ng C, Wang X J, Li S W, Zhu X R, Yu Y W, Zhang S Y. 2024. Combined analysis of transcriptomics with metabolomics provides insights into the resistance mechanism in winter jujube using L-methionine. Plant Physiology and Biochemistry, 214, 108951.

Zhang T, Wang Y, Ma X J, Ouyang Z P, Deng L, Shen S S, Dong X X, Du N S, Dong H, Guo Z H, Meng G, Piao F Z, Sun K L. 2022. Melatonin alleviates copper toxicity via improving ROS metabolism and antioxidant defense response in tomato seedlings. Antioxidants, 11, 758.

Zhang X, Chen B W, Wang L S, Ali S, Guo Y L, Liu J X, Wang J, Xie L N, Zhang Q Z. 2021. Genome-wide identification and characterization of caffeic acid O-methyltransferase gene family in soybean. Plants, 10, 2816.

Zhao B, Yu H, Liu D, Wang J Q, Feng X, He F M, Qi T S, Du C, Wang L L, Wang H F, Li F L. 2023. Combined transcriptome and metabolome analysis reveals adaptive defense responses to DON induction in potato. International Journal of Molecular Sciences, 24, 8054.

Zhao D K, Yao Z P, Zhang J M, Zhang R J, Mou Z M, Zhang X, Li Z H, Feng X L, Chen S Y, Reiter R J. 2021. Me latonin synthesis genes N-acetylserotonin methyltransferases evolved into caffeic acid O-methyltransferases and both assisted in plant terrestrialization. Journal of Pineal Research, 71, e12737.

Zhao X C, Xu Y Q, He F M, Sun M L, Yuan Q, Wang X, Kong D X, Liu D, Feng Y Z, Chen H S, Tian M, Liu D, Li F L. 2020. Low concentration of vomitoxin as elicitor induced resistance of dry rot disease of potato and its mechanism. Acta Agronomica Sinica, 46, 1801–1809. (in Chinese)

Zöngür A. 2024. Effi cacy of Beta vulgaris essential oils against potato dry rot disease and deoxynivalenol (DON) mycotoxin. Potato Research, 68, 355–365.

Zubieta C, Kota P, Ferrer J L, Dixon R A, Noel J P. 2002. Structural basis for the modulation of lignin monomer methylation by caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase. The Plant Cell, 14, 1265–1277.

Zuckerkandl E, Pauling L B. 1965. Evolutionary divergence and convergence in proteins. Evolving Genes and Proteins, 1965, 97–166.

Zuo D Y, Yi S Y, Liu R J, Qu B, Huang T, He W J, Li C, Li H P, Liao Y C. 2016. A deoxynivalenol-activated methionyl-tRNA synthetase gene from wheat encodes a nuclear localized protein and protects plants against Fusarium pathogens and mycotoxins. Phytopathology, 106, 614–623.

StCOMT1通过调节香豆素相关物质积累和ROS系统增强马铃薯对拟枝孢镰刀菌的抗性

StCOMT1 enhances potato resistance to Fusarium sporotrichioides by regulating coumarin-related substance accumulation and the ROS system

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics