NIN样蛋白4的自然变异与芥菜型油菜中氮利用效率的差异相关

doi:10.1016/j.jia.2026.05.001

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (10): 4351-4365.DOI: 10.1016/j.jia.2026.05.001

NIN样蛋白4的自然变异与芥菜型油菜中氮利用效率的差异相关

收稿日期:2025-09-09 修回日期:2026-05-01 接受日期:2026-03-19 出版日期:2026-10-20 发布日期:2026-09-21

Natural variation in NIN-LIKE PROTEIN 4 is associated with divergent nitrogen use efficiency in Brassica juncea

Jian Zeng^1*, Rumeng Wang^1*, Shiyao Cui¹, Xu Wang¹, Haixing Song^{1, 2}, Pan Gong^{1, 2#}, Zhenhua Zhang^{1, 2#}

¹College of Resources, Hunan Agricultural University, Changsha 410128, China
²Yuelushan Laboratory, Changsha 410128, China

Received:2025-09-09 Revised:2026-05-01 Accepted:2026-03-19 Online:2026-10-20 Published:2026-09-21
About author:Jian Zeng, E-mail: 1214223485@qq.com; Rumeng Wang, E-mail: 15526461183@163.com; #Correspondence Pan Gong, E-mail: gongpan309@163.com; Zhenhua Zhang, E-mail: zhzh1468@163.com * These authors contributed equally to this study.
Supported by:
This study was partially supported by the National Natural Science Foundation of China (32402666 and U21A20236), the Natural Science Foundation of Hunan Province, China (2025JJ60141), the Scientific Research Foundation of Education Bureau of Hunan Province, China (23B0203), the Major Project for Agricultural Biological Breeding, Ministry of Agriculture and Rural Affairs of China (2023ZD0404201), the Earmarked Fund for China Agriculture Research System (CARS-01-30), and the Innovation Foundation for Graduate of Hunan Province, China (CX20240657).

摘要/Abstract

摘要：

过度施用氮肥虽能提高作物产量，却以牺牲生态系统健康为代价，因此提升氮素利用效率已成为可持续农业发展的迫切需求。本研究以高氮效基因型H158和低氮效基因型L159为材料，探究芥菜型油菜响应低硝态氮胁迫的生理与分子机制，以发掘可用于改良氮效率的遗传资源。水培实验表明，在低硝态氮条件下，H158相较于L159表现出更强的抗氧化能力、氮同化能力及氮素吸收效率。田间试验进一步验证了上述结果：在不同施氮水平下，H158均具有更高的产量和农学氮肥利用率。基因组比较分析鉴定出1,654个与氮效率相关的基因，其中包括NIN-LIKE PROTEIN 4（BjuB04.NLP4）。该基因启动子区的G/C变异影响其转录调控功能，并调控根冠比对氮素供应的响应。值得注意的是，BjuB04.NLP4-HAP2单倍型（H158）在低氮土壤中占主导地位，而BjuB04.NLP4-HAP1单倍型（L159）更常见于高氮土壤。综上，本研究发掘并解析了可用于培育高氮效油菜品种的宝贵遗传资源，同时提供了优良种质和功能性分子标记。

Abstract:

While excessive nitrogen fertilizer application enhances crop yields, it does so at the expense of ecosystem health, making the enhancement of nitrogen use efficiency (NUE) an imperative for sustainable agriculture. This study investigates the mechanisms underlying the response to low nitrate (LN) stress in two Brassica juncea genotypes: a high-NUE (H158) and a low-NUE (L159), aiming to identify genetic resources for improved NUE. Hydroponic experiments revealed that H158 displayed enhanced antioxidant capacity, nitrogen assimilation, and nitrogen uptake efficiency (NUpE) under LN conditions relative to L159. Field trials corroborated these findings, with H158 demonstrating higher yields and greater agronomic nitrogen use efficiency (ANUE) across a gradient of nitrogen application rates. Genomic analysis identified 1,654 genes associated with NUE, including NIN-LIKE PROTEIN 4 (BjuB04.NLP4), whose G/C variants influenced transcriptional regulation and root-to-shoot ratio responses to nitrogen availability. Notably, BjuB04.NLP4-HAP2 (H158) was predominant in low-nitrogen soils, while BjuB04.NLP4-HAP1 (L159) was more common in high-nitrogen soils. Collectively, our findings uncover and characterize valuable genetic resources for breeding rapeseed varieties with enhanced NUE, providing both elite germplasm and functional molecular markers.

Key words: Brassica juncea , nitrogen use efficiency , BjuB04.NLP4 , haplotype , genotype

Jian Zeng, Rumeng Wang, Shiyao Cui, Xu Wang, Haixing Song, Pan Gong, Zhenhua Zhang. NIN样蛋白4的自然变异与芥菜型油菜中氮利用效率的差异相关[J]. Journal of Integrative Agriculture, 2026, 25(10): 4351-4365.

Jian Zeng, Rumeng Wang, Shiyao Cui, Xu Wang, Haixing Song, Pan Gong, Zhenhua Zhang. Natural variation in NIN-LIKE PROTEIN 4 is associated with divergent nitrogen use efficiency in Brassica juncea[J]. Journal of Integrative Agriculture, 2026, 25(10): 4351-4365.

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NIN样蛋白4的自然变异与芥菜型油菜中氮利用效率的差异相关

Natural variation in NIN-LIKE PROTEIN 4 is associated with divergent nitrogen use efficiency in Brassica juncea

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