高低产蛋鹅种的比较基因组学研究揭示ESR1在鹅产蛋能力中的重要作用

doi:10.1016/j.jia.2023.05.028

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (3): 1167-1180.DOI: 10.1016/j.jia.2023.05.028

高低产蛋鹅种的比较基因组学研究揭示ESR1在鹅产蛋能力中的重要作用

收稿日期:2023-02-23 接受日期:2023-04-21 出版日期:2025-03-20 发布日期:2025-02-28

Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying abilit

Qingyuan Ouyang^{1, 2}^*, Hengli Xie^{1, 2}^*, Shenqiang Hu^{1, 2}^*, Cong Lan^{1, 2}, Mingxia Ran^{1, 2}, Jiwei Hu^{1, 2}, Hua He^{1, 2}, Liang Li^{1, 2}, Hehe Liu^{1, 2}, Hao Qu², Jiwen Wang^{1, 2}^#

¹Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China
² State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, China

Received:2023-02-23 Accepted:2023-04-21 Online:2025-03-20 Published:2025-02-28
About author:Qingyuan Ouyang, E-mail: oyqy222@163.com; #Correspondence Jiwen Wang, Tel: +86-28-86291010, E-mail: wjw2886166@163.com * These authors contributed equally to this study.
Supported by:
This research was supported by the China Agriculture Research System of MOF and MARA (CARS-42-4), School Cooperation Project of Ya’an (21SXHZ0028), the Key Technology Support Program of Sichuan Province, China (2021YFYZ0014), for the financial support.

摘要/Abstract

摘要：

鹅较低的产量极大地限制了鹅产业的发展。中国拥有最丰富的鹅品种资源。本研究利用鸿雁及其驯化而来的高产和低产地方鹅种的基因组重测序数据，鉴定了与鹅产蛋能力相关的关键基因，并验证了它们的功能。选择性清除分析揭示，在驯化过程中与低产鹅种相比，416个基因在高产鹅种中受到了特异性选择。此外， GWAS分析的结果表明，RTCB、BPIFC、SYN3、SYNE1、VIP和ESR1可能与鹅产蛋能力的差异有关。值得注意的是，只有ESR1同时被GWAS和选择性清除分析两种方法鉴定出来。位于ESR1下游的Indel_{chr3:54429172}位点的基因型被证实会影响ESR1在卵巢基质中的表达，并与首次产卵时的体重和鹅的产蛋频率显著相关。CCK-8、EdU和流式细胞实验证实，ESR1可以促进鹅等级前卵泡颗粒细胞（phGCs）的凋亡并抑制其增殖。结合转录组数据，发现ESR1参与鹅phGCs的功能可能与MAPK和TGF-β信号通路有关。总的来说，我们的研究使用了来自不同鹅品种的基因组信息来确定ESR1下游的一个indel位点可能与鹅产蛋密切相关。通过细胞生物学和转录组学方法鉴定了ESR1参与鹅产蛋能力调控的主要途径和生物学过程。这些结果有助于进一步了解鹅的产蛋能力特征，提高鹅的产蛋量。

Abstract:

The low egg production of goose greatly limits the development of the industry. China possesses the most abundant goose breeds resources. In this study, genome resequencing data of swan goose (Anser cygnoides) and domesticated high and low laying goose breeds (Anser cygnoides domestiation) were used to identify key genes related to egg laying ability in geese and verify their functions. Selective sweep analyses revealed 416 genes that were specifically selected during the domestication process from swan geese to high laying geese. Furthermore, SNPs and Indels markers were used in GWAS analyses between high and low laying breed geese. The results showed that RTCB, BPIFC, SYN3, SYNE1, VIP, and ESR1 may be related to the differences in laying ability of geese. Notably, only ESR1 was identified simultaneously by GWAS and selective sweep analysis. The genotype of Indel_{chr3:54429172}, located downstream of ESR1, was confirmed to affect the expression of ESR1 in the ovarian stroma and showed significant correlation with body weight at first egg and laying frequency of geese. CCK-8, EdU, and flow cytometry confirmed that ESR1 can promote the apoptosis of goose pre-hierarchical follicles ganulosa cells (phGCs) and inhibit their proliferation. Combined with transcriptome data, it was found ESR1 involved in the function of goose phGCs may be related to MAPK and TGF-beta signaling pathways. Overall, our study used genomic information from different goose breeds to identify an indel located in the downstream of ESR1 associated with goose laying ability. The main pathways and biological processes of ESR1 involved in the regulation of goose laying ability were identified by cell biology and transcriptomics methods. These results are helpful to further understand the laying ability characteristics of goose and improve the egg production of geese.

Key words: goose , laying ability , genome , ESR1

Qingyuan Ouyang, Hengli Xie, Shenqiang Hu, Cong Lan, Mingxia Ran, Jiwei Hu, Hua He, Liang Li, Hehe Liu, Hao Qu, Jiwen Wang. 高低产蛋鹅种的比较基因组学研究揭示ESR1在鹅产蛋能力中的重要作用[J]. Journal of Integrative Agriculture, 2025, 24(3): 1167-1180.

Qingyuan Ouyang, Hengli Xie, Shenqiang Hu, Cong Lan, Mingxia Ran, Jiwei Hu, Hua He, Liang Li, Hehe Liu, Hao Qu, Jiwen Wang. Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying abilit[J]. Journal of Integrative Agriculture, 2025, 24(3): 1167-1180.

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高低产蛋鹅种的比较基因组学研究揭示ESR1在鹅产蛋能力中的重要作用

Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying abilit

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