Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (3): 1001-1016.DOI: 10.1016/j.jia.2023.11.031
长雄野生稻不同组织来源的内生细菌可促进多年生稻的生长发育
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收稿日期:2023-06-10接受日期:2023-10-16出版日期:2025-03-20发布日期:2025-02-28
Endophytic bacteria in different tissue compartments of African wild rice (Oryza longistaminata) promote perennial rice growth
Rui Tang*, Qinglin Tian*, Shuang Liu, Yurui Gong, Qingmao Li, Rui Chen, Linglin Wang, Fengyi Hu#, Liyu Huang#, Shiwen Qin#
Key Laboratory of Biology and Germplasm Innovation of Perennial Rice, Ministry of Agriculture and Rural Affairs/School of Agriculture, Yunnan University, Kunming 650091, China
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Received:2023-06-10Accepted:2023-10-16Online:2025-03-20Published:2025-02-28 -
About author:Rui Tang, E-mail: ruitang@mail.ynu.edu.cn; Qinglin Tian, E-mail: tql064897@163.com; #Correspondence Shiwen Qin, Tel: +86-871-65031539, E-mail: shiwenqin@ynu.edu.cn; Fengyi Hu, E-mail: hfengyi@ynu.edu.cn; Liyu Huang, E-mail: lyhuang@ynu.edu.cn * These authors contributed equally to this study. -
Supported by:This work was supported by funding from the National Natural Science Foundation of China (32060593 and 32060474), the Yunnan Provincial Science and Technology Department, China (202101AT070021 and 202101AS070001), the Yunnan Provincial Department of Education Science Research Fund Project, China (2023J0006), and the Graduate Innovation Project of Yunnan University, China (KC-22223012 and ZC-22222760).
摘要：
长雄野生稻（Oryza longistaminata）是一种来源于非洲且具有优良性状的野生稻，是多年生稻多年生性的供体。植物内生细菌对寄主的健康、适应性进化和胁迫耐受能力具有重要作用。然而，长雄野生稻内生细菌的群落结构及其对衍生后代多年生稻的促生作用目前还未知。本研究利用16S rRNA扩增子测序技术分析了长雄野生稻根、茎、叶组织中内生细菌的多样性、物种组成和网络结构。结果表明，长雄野生稻为内生细菌提供了不同的组织生态位，其中根部内生细菌群的结构和功能相较于茎部和叶部内生细菌群更为复杂且功能多样化。不同组织中的内生细菌群具有代表性的生物标记细菌属，如根部内生细菌生物标记为Paludibaculum、Pseudactinotalea、Roseimarinus等；茎部内生细菌生物标记为Blautia；叶部内生细菌生物标记为Lachnospiraceae NK4A136。长雄野生稻内生细菌网络包括了多种代谢功能，主要为生物降解与利用、解毒、代谢产物前体和能量的产生、甘氨酸途径、大分子修饰和代谢等。通过对长雄野生稻内生细菌的分离、筛选和功能鉴定，共发现163株内生细菌菌株具有解钾、溶磷、固氮、铁载体、产吲哚乙酸和1-氨基环丙烷-1-羧酸脱氨酶的促生功能。其中，鉴定为阴沟肠杆菌（Enterobacter cloacae）、路氏肠杆菌（Enterobacter ludwigii）、嗜麦芽窄食单胞菌（Stenotrophomonas maltophilia）、居泉沙雷氏菌（Serratia fonticola）和贝莱斯芽孢杆菌（Bacillus velezensis）的11个菌株对苗期的多年生稻具有较强定殖能力和促生效果。接种以上菌株的多年生稻形成了强壮的根系，并提高了光合效率、生物质量和营养吸收能力。另外，2株阴沟肠杆菌菌株对多年生稻表现出了基因型依赖的促生作用。本研究结果揭示了长雄野生稻的内生细菌微生态及其作用，表明长雄野生稻具有丰富的内生细菌资源，可开发为微生物菌肥，契合多年生稻绿色、可持续性的栽培模式。
引用本文
Rui Tang, Qinglin Tian, Shuang Liu, Yurui Gong, Qingmao Li, Rui Chen, Linglin Wang, Fengyi Hu, Liyu Huang, Shiwen Qin. 长雄野生稻不同组织来源的内生细菌可促进多年生稻的生长发育[J]. Journal of Integrative Agriculture, 2025, 24(3): 1001-1016.
Rui Tang, Qinglin Tian, Shuang Liu, Yurui Gong, Qingmao Li, Rui Chen, Linglin Wang, Fengyi Hu, Liyu Huang, Shiwen Qin. Endophytic bacteria in different tissue compartments of African wild rice (Oryza longistaminata) promote perennial rice growth[J]. Journal of Integrative Agriculture, 2025, 24(3): 1001-1016.
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