水稻OsNCED3和OsPYL1通过内源脱落酸调控糖转运蛋白促进颖花的闭合

doi:10.1016/j.jia.2023.11.004

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (2): 441-452.DOI: 10.1016/j.jia.2023.11.004

水稻OsNCED3和OsPYL1通过内源脱落酸调控糖转运蛋白促进颖花的闭合

收稿日期:2023-06-09 接受日期:2023-09-28 出版日期:2025-02-20 发布日期:2025-01-21

OsNCED3 and OsPYL1 promote the closure of rice florets by regulating sugar transporters through endogenous abscisic acid

Xiawan Zhai^{1, 2}, Wenbin Kai^{1, 2}, Youming Huang³, Jinyin Chen²^#, Xiaochun Zeng^{1, 3}^#

¹Jiangxi Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Jiangxi Agricultural University, Nanchang 330045, China
²College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
³ College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China

Received:2023-06-09 Accepted:2023-09-28 Online:2025-02-20 Published:2025-01-21
About author:Xiawan Zhai, E-mail: zhaixiawan@163.com; #Correspondence Jinyin Chen, Tel: +86-791-83813185, E-mail: jinyinchen@163.com; Xiaochun Zeng, Tel: +86-795-3200698, E-mail xchzeng2013@163.com
Supported by:
This study was supported by the National Natural Science Foundation of China (32260780 and 31360297), the China Postdoctoral Science Foundation (2021M701513), the Jiangxi 2011 Collaborative Innovation Centre of Postharvest Key Technology and Quality Safety of Fruits and Vegetables, China (JXGS-05), and the Gan Po 555 Engineering Excellence Talents Project in Jiangxi Province, China.

摘要/Abstract

摘要：

水稻作为世界第一大粮食作物，在杂交制种中常遇到父母本花时不遇的问题，且母本的颖花关闭慢会导致种子发霉，影响品质。植物激素脱落酸（ABA）在植物响应非生物胁迫中起着至关重要的作用。前人研究表明，外源ABA能够促进颖花关闭，然而其分子机制以及内源脱落酸对颖花关闭的作用尚不清楚。本研究通过改变水稻OsNCED3和OsPYL1的表达水平，探究内源ABA水平的改变对颖花关闭时间的影响，以及ABA通过糖转运蛋白对颖花关闭的作用机理。结果表明，过表达OsNCED3能够提高颖花内源脱落酸水平，从而使颖花关闭比野生型提前5.91分钟，OsNCED3下调则使颖花关闭推迟5.08分钟；进一步发现OsPYL1能够调控内源脱落酸水平同时改变对ABA敏感性，从而使过表达和敲除该基因的颖花关闭时间分别提前9.84分钟和延迟12.78分钟，且导致敲除株系种子裂颖率提高至15.4%。通过qRT-PCR检测发现，ABA对颖花关闭的促进作用可能是通过信号通路下游糖转运蛋白的响应来完成的，且OsPYL1与OsSWEET4能够在细胞膜上发生相互作用。以上结果表明，脱落酸能够促进水稻颖花关闭，增强植物对ABA的敏感性会促进该效果，其分子机制主要与下游响应ABA信号通路的糖转运蛋白相关，特别是OsSWEET4。

Abstract:

Rice is the world’s largest food crop, but it often encounters flowering asynchronization problems during hybrid rice seed production. In addition, the slow closure of female florets leads to seed mildew and affects the quality. The hormone abscisic acid (ABA) plays a crucial role in plant responses to abiotic stresses. Previous studies showed that exogenous ABA promotes floret closure, although the molecular mechanisms and effects of endogenous ABA on floret closure remain unknown. In this study, the effect of endogenous ABA on floret closure and the molecular mechanism by which ABA promotes floret closure through sugar transporters were investigated by changing the expression levels of OsNCED3 and OsPYL1 in rice. The results showed that overexpression (OE)-OsNCED3 increased the endogenous ABA level of florets. Florets closed 5.91 min earlier and OsNCED3 gene knockout line delayed the closure of florets by 5.08 min compared with the wild type. In addition, OsPYL1 regulated the endogenous ABA content and changed the sensitivity to ABA such that the floret closure times for OE and CRISPR-Cas9 (CR) were 9.84 min earlier and 12.78 min later, respectively, resulting in an increase in the split husk rate to 15.4%. The gene expression levels of some sugar transporters (STs) changed. The OsPYL1 and OsSWEET4 proteins could interact on the cell membrane. These results indicate that ABA promotes the closure of rice florets and the enhanced sensitivity to ABA promotes this effect even more. The molecular mechanism is mainly related to downstream sugar transporters that respond to the ABA signaling pathway, especially OsSWEET4.

Key words: rice , floret closure , sugar transporter , abscisic acid , seed quality

Xiawan Zhai, Wenbin Kai, Youming Huang, Jinyin Chen, Xiaochun Zeng. 水稻OsNCED3和OsPYL1通过内源脱落酸调控糖转运蛋白促进颖花的闭合[J]. Journal of Integrative Agriculture, 2025, 24(2): 441-452.

Xiawan Zhai, Wenbin Kai, Youming Huang, Jinyin Chen, Xiaochun Zeng. OsNCED3 and OsPYL1 promote the closure of rice florets by regulating sugar transporters through endogenous abscisic acid[J]. Journal of Integrative Agriculture, 2025, 24(2): 441-452.

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水稻OsNCED3和OsPYL1通过内源脱落酸调控糖转运蛋白促进颖花的闭合

OsNCED3 and OsPYL1 promote the closure of rice florets by regulating sugar transporters through endogenous abscisic acid

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