CONSTANS-LIKE（COL）家族全基因组鉴定及PpCOL8通过应答水杨酸与PpMADS相互作用调控砂梨果实衰老

doi:10.1016/j.jia.2024.01.011

摘要/Abstract

摘要： “黄金梨”属于砂梨系统，营养品质和口感极佳。然而，由于其货架期短，“黄金梨”产业化发展受到严重限制。水杨酸（Salicylic acid, SA）是一种植物激素，可以延缓果实衰老从而延长货架期。但是，SA在果实衰老过程中如何调控CONSTANS-LIKE基因（COLs）以及COL基因在砂梨果实衰老中的调控作用尚不清楚。本研究在砂梨中鉴定出22个COL基因，包括通过转录组鉴定的4个COL基因（PpCOL8、PpCOL9a、PpCOL9b和PpCOL14）和通过全基因组分析鉴定的18个COL基因。根据COL蛋白的结构域，将这些COL基因分为三个亚家族。PpCOL8具有两个B-box基序和一个CCT结构域，属于第一亚家族。相比之下，其他三个PpCOLs，PpCOL9a，PpCOL9b和PpCOL14，具有相似的保守蛋白结构域和基因结构，属于第三亚家族。PpCOL8、PpCOL9a、PpCOL9b和PpCOL14在梨各组织中均表现出不同的表达模式，且均在果实发育早期优势表达。此外，外源SA处理抑制了PpCOL8的表达，却上调了PpCOL9a和PpCOL9b的表达。有趣的是，PpCOL8与PpMADS相互作用，而PpMADS是一个MADS-box蛋白基因，在果实中优势表达且SA上调其表达。在过表达PpCOL8基因的砂梨果实中，乙烯生成量和丙二醛（MDA）含量均增加；而且砂梨果实中抗氧化酶（POD和SOD）活性以及PpPOD1和PpSOD1的表达量均下调，表明PpCOL8促进砂梨果实衰老。而在过表达PpMADS的砂梨果实中，相应的变化则相反，说明PpMADS延缓砂梨果实衰老。PpCOL8和PpMADS的共转化亦延缓砂梨果实衰老。本研究揭示了PpCOL8通过SA信号通路与PpMADS相互作用在梨果实衰老中起关键作用。

Abstract: Pyrus pyrifolia Nakai ‘Whangkeumbae’ is a sand pear fruit with excellent nutritional quality and taste. However, the industrial development of pear fruit is significantly limited by its short shelf life. Salicylic acid (SA), a well-known phytohormone, can delay fruit senescence and improve shelf life. However, the mechanism by which SA regulates CONSTANS-LIKE genes (COLs) during fruit senescence and the role of COL genes in mediating fruit senescence in sand pear are poorly understood. In this study, 22 COL genes were identified in sand pear, including four COLs (PpCOL8, PpCOL9a, PpCOL9b, and PpCOL14) identified via transcriptome analysis and 18 COLs through genome-wide analysis. These COL genes were divided into three subgroups according to the structural domains of the COL protein. PpCOL8, with two B-box motifs and one CCT domain, belonged to the first subgroup. In contrast, the other three PpCOLs, PpCOL9a, PpCOL9b, and PpCOL14, with similar conserved protein domains and gene structures, were assigned to the third subgroup. The four COLs showed different expression patterns in pear tissues and were preferentially expressed at the early stage of fruit development. Moreover, the expression of PpCOL8 was inhibited by exogenous SA treatment, while SA up-regulated the expression of PpCOL9a and PpCOL9b. Interestingly, PpCOL8 interacts with PpMADS, a MADS-box protein preferentially expressed in fruit, and SA up-regulated its expression. While the production of ethylene and the content of malondialdehyde (MDA) were increased in PpCOL8-overexpression sand pear fruit, the antioxidant enzyme (POD and SOD) activity and the expression of PpPOD1 and PpSOD1 in the sand pear fruits were down-regulated, which showed that PpCOL8 promoted sand pear fruit senescence. In contrast, the corresponding changes were the opposite in PpMADS-overexpression sand pear fruits, suggesting that PpMADS delayed sand pear fruit senescence. The co-transformation of PpCOL8 and PpMADS also delayed sand pear fruit senescence. The results of this study revealed that PpCOL8 can play a key role in pear fruit senescence by interacting with PpMADS through the SA signaling pathway.

Key words: Pyrus pyrifolia , CONSTANS-LIKE gene , salicylic acid , fruit senescence , MADS

. CONSTANS-LIKE（COL）家族全基因组鉴定及PpCOL8通过应答水杨酸与PpMADS相互作用调控砂梨果实衰老[J]. Journal of Integrative Agriculture, 2024, 23(4): 1222-1237.

Yue Xu, Shurui Song, Huiying Wang, Xilong Cao, Xinran Zhao, Wenli Wang, Liyue Huo, Yawei Li, Misganaw Wassie, Bin Lu, Liang Chen, Haiyan Shi.

Genome-wide identification of the CONSTANS-LIKE (COL) family and mechanism of fruit senescence regulation by PpCOL8 in sand pear (Pyrus pyrifolia) [J]. Journal of Integrative Agriculture, 2024, 23(4): 1222-1237.

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