Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (11): 3084-3088.DOI: 10.1016/S2095-3119(21)63631-6

所属专题： 动物医学合辑Veterninary Medicine

• 论文 • 上一篇    

  

  • 收稿日期:2020-09-04 出版日期:2021-11-01 发布日期:2021-09-17

Viricidal activity of several disinfectants against African swine fever virus

JIANG Cheng-gang1, 2, SUN Ying2, ZHANG Fan2, AI Xin2, FENG Xiao-ning2, HU Wei1, ZHANG Xian-feng1, 2, ZHAO Dong-ming1, BU Zhi-gao1, 2, HE Xi-jun1, 2 
  

  1. 1 State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China
    2 National High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, P.R.China
  • Received:2020-09-04 Online:2021-11-01 Published:2021-09-17
  • Contact: Correspondence ZHAO Dong-ming, E-mail: zhaodongming@caas.cn; BU Zhi-gao, E-mail: buzhigao@caas.cn; HE Xi-jun, E-mail: hexijun@caas.cn
  • About author:JIANG Cheng-gang, E-mail: jiangchenggang01@caas.cn;
  • Supported by:
    This work was supported by the National Key R&D Program of China (2018YFC1200600), the grant from the State Key Laboratory of Veterinary Biotechnology Program (SKLVBP201801), and the National Science and Technology Major Project of China (2018ZX10734401-018-002).

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摘要：

本研究以表达eGFP重组非洲猪瘟病毒为指示病毒，通过体外细胞培养方法，模拟浸泡和喷雾方式，在室温条件下，观察消毒剂不同浓度、不同时间作用后是否产生可见荧光信号为标准，来判定病毒是否完全被消毒剂灭活。结果显示，模拟浸泡消毒方式，在室温条件下，碘酸混合溶液0.5%浓度，作用10分钟能完全灭活非洲猪瘟病毒；过硫酸氢钾复合粉0.25%浓度作用30分钟能完全灭活非洲猪瘟病毒；枸橼酸粉0.25%浓度作用30分钟能完全灭活非洲猪瘟病毒；二氯异氰脲酸钠粉0.125%浓度作用60分钟能完全灭活非洲猪瘟病毒；戊二醛癸甲溴铵溶液0.2%浓度作用60分钟能完全灭活非洲猪瘟病毒；癸甲溴铵溶液0.5%浓度作用60分钟能完全灭活非洲猪瘟病毒。模拟喷雾消毒方式，在室温条件下，碘酸混合溶液0.5%浓度，作用10分钟能完全灭活非洲猪瘟病毒；过硫酸氢钾复合粉0.25%浓度作用60分钟能完全灭活非洲猪瘟病毒；枸橼酸粉0.5%浓度作用30分钟能完全灭活非洲猪瘟病毒；二氯异氰脲酸钠粉0.25%浓度作用60分钟能完全灭活非洲猪瘟病毒；戊二醛癸甲溴铵溶液0.2%浓度作用60分钟能完全灭活非洲猪瘟病毒；癸甲溴铵溶液0.5%浓度作用60分钟能完全灭活非洲猪瘟病毒。这些结果表明，常用市售6种消毒剂，在实验室条件下，不同浓度和作用时间均能有效灭活非洲猪瘟病毒，本实验建立了一种应用表达eGFP重组非洲猪瘟病毒为指示病毒，快速准确评价消毒剂灭活非洲猪瘟病毒效果的方法，为非洲猪瘟的生物安全防控提供有力理论依据和技术支持。


Abstract:

Prevention of African swine fever, a disease caused by African swine fever virus (ASFV), requires maintenance of high biosecurity standards, which principally relies on disinfection.  Finding the perfect disinfectant against ASFV is difficult because of the lack of relevant data.  Therefore, we aimed to find the most effective disinfectant and to optimise its concentration as well as contact time to confirm the viricidal effect against ASFV in vitro.  We evaluated the viricidal activity of three concentrations each of six common disinfectants against ASFV using immersion disinfection assay (IDA) and spray disinfection assay (SDA); the concentrations of these disinfectants at which complete viral inactivation occurred were almost same as the manufacturer-recommended concentrations, but the exposure times for viral inactivation are different.  The following disinfectants (assay: concentration, exposure time) showed complete inactivation: iodine and acid mixed solution (IDA/SDA: 0.5%, 10 min); compound potassium peroxymonosulfate (IDA: 0.25%, 30 min; SDA: 0.25%, 60 min); citric acid (IDA: 0.25%, 60 min; SDA: 0.5%, 60 min); sodium dichloroisocyanurate (IDA: 0.125%, 60 min; SDA: 0.25%, 60 min); and glutaral ang deciquam (IDA/SDA: 0.2%, 60 min); and deciquam (IDA/SDA: 0.5%, 60 min).  However, in the presence of organic material contamination, disinfectants did not show a marked inactivation effect.  Therefore, disinfection procedures should be performed in two steps: thorough mechanical cleaning followed by application of disinfectant.  In conclusion, all the tested disinfectants can inactivate ASFV; these can be used as alternative disinfectants to enhance biosecurity.
 

Key words: disinfection ,  African swine fever virus (ASFV) ,  virucidal effects ,  biosecurity