Identification of quantitative trait loci and candidate genes controlling seed pigments of rapeseed

doi:10.1016/S2095-3119(20)63377-9

摘要/Abstract

摘要：

甘蓝型油菜是食用植物油和饲用蛋白的重要来源，然而种子中色素严重影响菜籽油的品质和饼粕的饲用价值。本研究以甘蓝型油菜黄籽母本GH06和黑籽父本中油821构建的重组自交系群体为研究对象，对不同环境下种子（种胚和种皮）色素组份进行QTL定位分析和候选基因的鉴定。结果共检测到94个影响种皮和种胚色素组份含量的QTL位点，44个在种胚中被检测到，50个在种皮中被检测到，分别位于甘蓝型油菜15条不同染色体上。其中包括28个花色素含量相关的QTL，单个QTL可解释2.41-44.46％的表型变异；24个类黄酮含量相关的QTL，单个QTL可解释2.41-20.26％的表型变异；16个总酚含量相关的QTL，单个QTL可解释2.74–23.68％的表型变异；26个黑色素含量相关的QTL，单个QTL可解释表型变异的2.37–24.82％，说明这些性状是由多基因控制的数量性状。同时，在A06，A09和C08染色体上存在多个QTL集中分布的现象，分别包含15个、19个和10个色素相关的QTL，且大多数QTL解释的表型变异>10%被认为是主效QTL。根据甘蓝型油菜“Darmor-bzh”参考基因组注释信息，在被重复检测到的QTL区间内筛选到67个候选基因，通过RNA-seq和qRT-PCR分析结果推断12个差异表达基因可能是参与种子色素合成相关的重要候选基因。本研究结果为甘蓝型油菜种子色素合成遗传机理提供了新的认识并为深入解析甘蓝型油菜粒色形成的分子机制奠定了基础。

Abstract:

Rapeseed (Brassica napus L.) is an important source of edible vegetable oil and feed protein; however, seed pigments affect the quality of rapeseed oil and the feed value of the residue from oil pressing. Here, we used a population of rapeseed recombinant inbred lines (RILs) derived from the black-seeded male parent cultivar Zhongyou 821 and the yellow-seeded female parent line GH06 to map candidate genes controlling seed pigments in embryos and the seed coat. We detected 94 quantitative trait loci (QTLs) for seed pigments (44 for embryos and 50 for seed coat), distributed over 15 of the 19 rapeseed chromosomes. These included 28 QTLs for anthocyanidin content, explaining 2.41–44.66% of phenotypic variation; 24 QTLs for flavonoid content, explaining 2.41–20.26% of phenotypic variation; 16 QTLs for total phenol content, accounting for 2.74–23.68% of phenotypic variation; and 26 QTLs for melanin content, accounting for 2.37–24.82% of phenotypic variation, indicating that these traits are under multigenic control. Consensus regions on chromosomes A06, A09 and C08 were associated with multiple seed pigment traits, including 15, 19 and 10 QTLs, respectively, most of which were major QTLs explaining >10% of the phenotypic variation. Based on the annotation of the B. napus “Darmor-bzh” reference genome, 67 candidate genes were predicted from these consensus QTLs regions, and 12 candidate genes were identified as potentially involved in pigment accumulation by RNA-seq and qRT-PCR analysis. These preliminary results provide insight into the genetic architecture of pigment biosynthesis and lay a foundation for exploring the molecular mechanisms underlying seed coat color in B. napus.

Key words: Brassica napus L. , embryo , seed coat , pigments , QTL

. [J]. Journal of Integrative Agriculture, 2021, 20(11): 2862-2879.

ZHU Mei-chen, HU Ran, ZHAO Hui-yan, TANG Yun-shan, SHI Xiang-tian, JIANG Hai-yan, ZHANG Zhi-yuan, FU Fu-you, XU Xin-fu, TANG Zhang-lin, LIU Lie-zhao, LU Kun, LI Jia-na, QU Cun-min. Identification of quantitative trait loci and candidate genes controlling seed pigments of rapeseed[J]. Journal of Integrative Agriculture, 2021, 20(11): 2862-2879.

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