Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (3): 502-511.DOI: 10.1016/S2095-3119(15)61129-7

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Characterization of Ppd-D1 alleles on the developmental traits and rhythmic expression of photoperiod genes in common wheat

 ZHAO Yong-ying, WANG Xiang, WEI Li, WANG Jing-xuan, YIN Jun   

  1. 1、National Engineering Research Center for Wheat/Collaborative Innovation Center of Henan Grain Crops/State Key Laboratory of
    Wheat and Maize Crop Science/College of Agriculture, Henan Agricultural University, Zhengzhou 450002, P.R.China
    2、National Laboratory of Wheat Engineering/Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-Huai Region,
    Ministry of Agricultural/Key Laboratory of Wheat Biology of Henan Province/Wheat Research Institution, Henan Academy of
    Agricultural Sciences, Zhengzhou 450002, P.R.China
  • 收稿日期:2015-02-11 出版日期:2016-03-07 发布日期:2016-03-09
  • 通讯作者: YIN Jun, Tel/Fax: +86-371-63558203, E-mail: xmzxyj@126.com
  • 作者简介:ZHAO Yong-ying, Mobile: +86-13513895016, Tel: +86-371-65750512, E-mail: yongying001@126.com;* These authors contributed equally to this study.
  • 基金资助:

    This research was supported by the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD16B07, 2013BAD04B01) and the National Natural Science Foundation of China (31271726).

Characterization of Ppd-D1 alleles on the developmental traits and rhythmic expression of photoperiod genes in common wheat

 ZHAO Yong-ying, WANG Xiang, WEI Li, WANG Jing-xuan, YIN Jun   

  1. 1、National Engineering Research Center for Wheat/Collaborative Innovation Center of Henan Grain Crops/State Key Laboratory of
    Wheat and Maize Crop Science/College of Agriculture, Henan Agricultural University, Zhengzhou 450002, P.R.China
    2、National Laboratory of Wheat Engineering/Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-Huai Region,
    Ministry of Agricultural/Key Laboratory of Wheat Biology of Henan Province/Wheat Research Institution, Henan Academy of
    Agricultural Sciences, Zhengzhou 450002, P.R.China
  • Received:2015-02-11 Online:2016-03-07 Published:2016-03-09
  • Contact: YIN Jun, Tel/Fax: +86-371-63558203, E-mail: xmzxyj@126.com
  • About author:ZHAO Yong-ying, Mobile: +86-13513895016, Tel: +86-371-65750512, E-mail: yongying001@126.com;* These authors contributed equally to this study.
  • Supported by:

    This research was supported by the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD16B07, 2013BAD04B01) and the National Natural Science Foundation of China (31271726).

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摘要： Photoperiodic response is an important characteristic that plays an important role in plant adaptability for various environments. Wheat cultivars grow widely and have high yield potential for the strong photoperiod adaptibility. To assess the photoperiodic response of different genotypes in wheat cultivars, the photoperiodic effects of the Ppd-D1 alleles and the expressions of the related TaGI, TaCO and TaFT genes in Liaochun 10 and Ningchun 36 were investigated under the short-day (6 h light, SD), moderate-day (12 h light, MD) and long-day (24 h light, LD) conditions. Amplicon length comparison indicated that the promoter of Ppd-D1 in Ningchun 36 is intact, while Liaochun 10 presented the partial sequence deletion of Ppd-D1 promoter. The durations of all developmental stages of the two cultivars were reduced by subjection to an extended photoperiod, except for the stamen and pistil differentiation stage in the Liaochun 10 cultivar. The expression levels of the Ppd-D1 alleles and the TaGI, TaCO and TaFT genes associated with the photoperiod pathway were examined over a 24-h period under SD and MD conditions. The relationships of different photoperiodic responses of the two cultivars and the expression of photoperiod pathway genes were analyzed accordingly. The photoperiod insensitive (PI) genotype plants flower early under SD; meanwhile, the abnormal expression of the Ppd-D1a allele is accompanied with an increase in TaFT1 expression and the TaCO expression variation. The results would facilitate molecular breeding in wheat.

关键词: wheat photoperiod , spike differentiation , heading , gene expression , Ppd-D1

Abstract: Photoperiodic response is an important characteristic that plays an important role in plant adaptability for various environments. Wheat cultivars grow widely and have high yield potential for the strong photoperiod adaptibility. To assess the photoperiodic response of different genotypes in wheat cultivars, the photoperiodic effects of the Ppd-D1 alleles and the expressions of the related TaGI, TaCO and TaFT genes in Liaochun 10 and Ningchun 36 were investigated under the short-day (6 h light, SD), moderate-day (12 h light, MD) and long-day (24 h light, LD) conditions. Amplicon length comparison indicated that the promoter of Ppd-D1 in Ningchun 36 is intact, while Liaochun 10 presented the partial sequence deletion of Ppd-D1 promoter. The durations of all developmental stages of the two cultivars were reduced by subjection to an extended photoperiod, except for the stamen and pistil differentiation stage in the Liaochun 10 cultivar. The expression levels of the Ppd-D1 alleles and the TaGI, TaCO and TaFT genes associated with the photoperiod pathway were examined over a 24-h period under SD and MD conditions. The relationships of different photoperiodic responses of the two cultivars and the expression of photoperiod pathway genes were analyzed accordingly. The photoperiod insensitive (PI) genotype plants flower early under SD; meanwhile, the abnormal expression of the Ppd-D1a allele is accompanied with an increase in TaFT1 expression and the TaCO expression variation. The results would facilitate molecular breeding in wheat.

Key words: wheat photoperiod , spike differentiation , heading , gene expression , Ppd-D1