Over-expression of GhDWF4 gene improved tomato fruit quality and accelerated fruit ripening

doi:10.1016/S2095-3119(15)61059-0

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (10): 1980-1991.DOI: 10.1016/S2095-3119(15)61059-0

Over-expression of GhDWF4 gene improved tomato fruit quality and accelerated fruit ripening

YE Shu-e, LI Fang, LI Xian-bi, HONG Qi-bin, ZHAI Yun-lan, HU Ming-yu, WEI Ting, DENG Sha-sha, PEI Yan, LUO Ming

1、Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture/Biotechnology Research Center,
Southwest University, Chongqing 400716, P.R.China
2、Citrus Research Institute, Chinese Academy of Agricultural Sciences/National Citrus Engineering Research Center, Chongqing
400712, P.R.China

收稿日期:2014-10-27 出版日期:2015-10-09 发布日期:2015-10-12
通讯作者: LUO Ming, Tel: +86-23-68250042, Fax: +86-23-68251883, E-mail: luo0424@126.com
作者简介:YE Shu-e, E-mail: 61730797@qq.com;* These authors contributed equally to this work.
基金资助:
This work was supported by the Natural Science Foundation of Chongqing, China (CSTC, 2011BB1007) and the Genetically Modified Organisms Breeding Major Projects of China (2009ZX08009-118B).

Over-expression of GhDWF4 gene improved tomato fruit quality and accelerated fruit ripening

YE Shu-e, LI Fang, LI Xian-bi, HONG Qi-bin, ZHAI Yun-lan, HU Ming-yu, WEI Ting, DENG Sha-sha, PEI Yan, LUO Ming

1、Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture/Biotechnology Research Center,
Southwest University, Chongqing 400716, P.R.China
2、Citrus Research Institute, Chinese Academy of Agricultural Sciences/National Citrus Engineering Research Center, Chongqing
400712, P.R.China

Received:2014-10-27 Online:2015-10-09 Published:2015-10-12
Contact: LUO Ming, Tel: +86-23-68250042, Fax: +86-23-68251883, E-mail: luo0424@126.com
About author:YE Shu-e, E-mail: 61730797@qq.com;* These authors contributed equally to this work.
Supported by:
This work was supported by the Natural Science Foundation of Chongqing, China (CSTC, 2011BB1007) and the Genetically Modified Organisms Breeding Major Projects of China (2009ZX08009-118B).

摘要/Abstract

摘要： Brassinosteroids (BRs), a class of steroidal phytohormones are essential for many biological processes in plant. However, little is known about their roles in fruit development. Tomato is a highly valuable vegetable and has been adopted as the model species for studying fruit growth, development, and ripening. To understand the role of endogenous BRs in the development of tomato fruit, the expression patterns of three homologues of DWF4 gene were investigated and the transgenic tomato plants were generated in which the GhDWF4 gene from upland cotton (Gossypium hirsutum L.) was ectopically expressed. The contents of main quality components were analyzed in fruits of transgenic tomato line and non-transgenic line (control plant, CP) when the fruit was mature. SlCYP90B3 that possesses high homology with GhDWF4 preferentially expressed in mature fruit. Significantly higher contents of soluble sugar, soluble proteins, and vitamin C were obtained in fruit of transgenic tomato lines compared with those in the CP. Furthermore, overexpressing GhDWF4 promoted fruit growth and ripening. The weight per fruit was increased by about 23% in transgenic lines. In addition, overexpressing GhDWF4 promoted the germination of transgenic tomato seeds and hypocotyl elongation of seedlings. These results indicated that overexpressing GhDWF4 gene in tomato could increase the contents of many nutrients in fruit and accelerate fruit ripening. It is suggested that increased endogenous BRs in fruit affect the growth and development of tomato fruit and therefore improved the nutrient quality of tomato.

关键词: brassinosteroids , GhDWF4 , tomato , fruit ripening , fruit quality , transgenic plant

Abstract: Brassinosteroids (BRs), a class of steroidal phytohormones are essential for many biological processes in plant. However, little is known about their roles in fruit development. Tomato is a highly valuable vegetable and has been adopted as the model species for studying fruit growth, development, and ripening. To understand the role of endogenous BRs in the development of tomato fruit, the expression patterns of three homologues of DWF4 gene were investigated and the transgenic tomato plants were generated in which the GhDWF4 gene from upland cotton (Gossypium hirsutum L.) was ectopically expressed. The contents of main quality components were analyzed in fruits of transgenic tomato line and non-transgenic line (control plant, CP) when the fruit was mature. SlCYP90B3 that possesses high homology with GhDWF4 preferentially expressed in mature fruit. Significantly higher contents of soluble sugar, soluble proteins, and vitamin C were obtained in fruit of transgenic tomato lines compared with those in the CP. Furthermore, overexpressing GhDWF4 promoted fruit growth and ripening. The weight per fruit was increased by about 23% in transgenic lines. In addition, overexpressing GhDWF4 promoted the germination of transgenic tomato seeds and hypocotyl elongation of seedlings. These results indicated that overexpressing GhDWF4 gene in tomato could increase the contents of many nutrients in fruit and accelerate fruit ripening. It is suggested that increased endogenous BRs in fruit affect the growth and development of tomato fruit and therefore improved the nutrient quality of tomato.

Key words: brassinosteroids , GhDWF4 , tomato , fruit ripening , fruit quality , transgenic plant

YE Shu-e, LI Fang, LI Xian-bi, HONG Qi-bin, ZHAI Yun-lan, HU Ming-yu, WEI Ting, DENG Sha-sha, PEI Yan, LUO Ming. Over-expression of GhDWF4 gene improved tomato fruit quality and accelerated fruit ripening[J]. Journal of Integrative Agriculture, 2015, 14(10): 1980-1991.

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Over-expression of GhDWF4 gene improved tomato fruit quality and accelerated fruit ripening

Over-expression of GhDWF4 gene improved tomato fruit quality and accelerated fruit ripening

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