CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi:10.1016/S2095-3119(13)60689-9

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (8): 1759-1765.DOI: 10.1016/S2095-3119(13)60689-9

CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

ZHANG Yan, WU Sachula, LUO Fen-hua, Baiyinbatu , LIU Lin-hong, HU Tian-yuan, YU Bo-yang, LI Guang-peng , WU Ying-ji

1、The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University,
Hohhot 010021, P.R.China
2、School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010021, P.R.China

收稿日期:2013-03-28 出版日期:2014-08-01 发布日期:2014-08-02
通讯作者: WU Ying-ji, Tel: +86-471-4992443, Mobile: 18686031623, E-mail: wuyj1211@163.com
作者简介:WU Ying-ji, Tel: +86-471-4992443, Mobile: 18686031623, E-mail: wuyj1211@163.com* These authors contributed equally to this study.
基金资助:
This work was supported by the Program for Innovative Research of Ministry of Education, China (IRT0833) and the Natural Science Foundation of Inner Mongolia, China (2009ZD05).

CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

ZHANG Yan, WU Sachula, LUO Fen-hua, Baiyinbatu , LIU Lin-hong, HU Tian-yuan, YU Bo-yang, LI Guang-peng , WU Ying-ji

1、The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University,
Hohhot 010021, P.R.China
2、School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010021, P.R.China

Received:2013-03-28 Online:2014-08-01 Published:2014-08-02
Contact: WU Ying-ji, Tel: +86-471-4992443, Mobile: 18686031623, E-mail: wuyj1211@163.com
About author:WU Ying-ji, Tel: +86-471-4992443, Mobile: 18686031623, E-mail: wuyj1211@163.com* These authors contributed equally to this study.
Supported by:
This work was supported by the Program for Innovative Research of Ministry of Education, China (IRT0833) and the Natural Science Foundation of Inner Mongolia, China (2009ZD05).

摘要/Abstract

摘要： Spermatogonial stem cells (SSCs) are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation and purification of the SSCs is challenging, especially for livestock animals. It has been confirmed that CDH1 (cadherin-1, also known as E-cadherin) can be expressed in undifferentiated SSCs of mouse and rats, but it has not been verified in sheep. Here, CDH1 was found as a novel surface marker for sheep SSCs. In this paper, sheep anti- CDH1 polyclonal antibodies were prepared and its activity was checked. Using the obtained antibodies and immunohistochemistry analysis, we confirmed that CDH1 can be expressed by SSCs in sheep testis.

关键词: sheep , spermatogonial stem cells , CDH1 , polyclonal antibodies , immunofluorescent staining

Abstract: Spermatogonial stem cells (SSCs) are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation and purification of the SSCs is challenging, especially for livestock animals. It has been confirmed that CDH1 (cadherin-1, also known as E-cadherin) can be expressed in undifferentiated SSCs of mouse and rats, but it has not been verified in sheep. Here, CDH1 was found as a novel surface marker for sheep SSCs. In this paper, sheep anti- CDH1 polyclonal antibodies were prepared and its activity was checked. Using the obtained antibodies and immunohistochemistry analysis, we confirmed that CDH1 can be expressed by SSCs in sheep testis.

Key words: sheep , spermatogonial stem cells , CDH1 , polyclonal antibodies , immunofluorescent staining

ZHANG Yan, WU Sachula, LUO Fen-hua, Baiyinbatu, LIU Lin-hong, HU Tian-yuan, YU Bo-yang, LI Guang-peng , WU Ying-ji. CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis[J]. Journal of Integrative Agriculture, 2014, 13(8): 1759-1765.

ZHANG Yan, WU Sachula, LUO Fen-hua, Baiyinbatu , LIU Lin-hong, HU Tian-yuan, YU Bo-yang, LI Guang-peng , WU Ying-ji. CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis[J]. Journal of Integrative Agriculture, 2014, 13(8): 1759-1765.

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CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

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