Use of the N-alkanes to Estimate Intake, Apparent Digestibility and Diet Composition in Sheep Grazing on Stipa breviflora Desert Steppe

doi:10.1016/S2095-3119(13)60502-X

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (5): 1065-1072.DOI: 10.1016/S2095-3119(13)60502-X

Use of the N-alkanes to Estimate Intake, Apparent Digestibility and Diet Composition in Sheep Grazing on Stipa breviflora Desert Steppe

HU Hong-lian, LIU Yong-zhi, LI Ya-kui, LU De-xun , GAO Min

1、Institute of Animal Nutrition and Feed, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, P.R.China
2、Inner Mongolia Prataculture Research Center, Chinese Academy of Sciences, Huhhot 010031, P.R.China
3、Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R.China
4、College of Animal and Technology, Hebei North University, Zhangjiakou 075000, P.R.China

收稿日期:2012-12-27 出版日期:2014-05-01 发布日期:2014-05-06
通讯作者: LIU Yong-zhi, Tel: +86-471-5295028, E-mail: liuyongzhi@vip.163.com
作者简介:HU Hong-lian, Mobile: 13947198168, E-mail: honglianhu2010@163.com
基金资助:
This study was financially supported in part by China Agriculture Research System (CARS-37).

Use of the N-alkanes to Estimate Intake, Apparent Digestibility and Diet Composition in Sheep Grazing on Stipa breviflora Desert Steppe

HU Hong-lian, LIU Yong-zhi, LI Ya-kui, LU De-xun , GAO Min

1、Institute of Animal Nutrition and Feed, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, P.R.China
2、Inner Mongolia Prataculture Research Center, Chinese Academy of Sciences, Huhhot 010031, P.R.China
3、Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R.China
4、College of Animal and Technology, Hebei North University, Zhangjiakou 075000, P.R.China

Received:2012-12-27 Online:2014-05-01 Published:2014-05-06
Contact: LIU Yong-zhi, Tel: +86-471-5295028, E-mail: liuyongzhi@vip.163.com
About author:HU Hong-lian, Mobile: 13947198168, E-mail: honglianhu2010@163.com
Supported by:
This study was financially supported in part by China Agriculture Research System (CARS-37).

摘要/Abstract

摘要： The application of n-alkane as markers to estimate herbage intake, apparent digestibility and species composition of diet consumed by grazing sheep was studied. Six local Mongolian sheep were used to determine dry matter (DM) intake, apparent DM digestibility and species composition of diet during summer, autumn and winter. Animals were orally dosed twice daily with n-alkane gelatin capsules containing 60 mg C32-alkane as an external marker. Diet composition was estimated by comparing the odd-chain n-alkanes pattern profile (C27-C31) of the consumed plant species with the n-alkanes fecal concentrations of grazing animals, using a non-negative least squares algorithm called EATWHAT software package. The alkane pair C32:C33 and C33 alkane were used to estimate DM intake and diet apparent DM digestibility, respectively. The results showed that daily dry matter intake of the sheep were 1.77, 1.61 and 1.18 kg d-1 in summer, autumn and winter, respectively. Apparent DM digestibility, crude protein (CP), metabolizable energy (ME) and neutral detergent fiber (NDF) intake of diet consumed by sheep decreased significantly (P<0.01) from summer to winter, with no evident changes in ADF and ADL intake. Diet composition indicated Artemisia frigida Willd was the most dominant diet component, contributed 79.68, 68.12 and 86.26% of sheep’s diets in summer, autumn and winter, respectively. Cleistogenes songorica Ohwi and Convolvulus ammannii Desr were the important components of the diet. Although Stipa breviflora Griseb is one of the main plant species in the study area, the sheep rarely choosed it. The study indicated that CP and ME in diet consumed by sheep were deficient in winter. Therefore, appropriate supplementation strategies should be indispensable during this period.

关键词: n-alkanes , diet composition , intake , apparent digestibility , sheep

Abstract: The application of n-alkane as markers to estimate herbage intake, apparent digestibility and species composition of diet consumed by grazing sheep was studied. Six local Mongolian sheep were used to determine dry matter (DM) intake, apparent DM digestibility and species composition of diet during summer, autumn and winter. Animals were orally dosed twice daily with n-alkane gelatin capsules containing 60 mg C32-alkane as an external marker. Diet composition was estimated by comparing the odd-chain n-alkanes pattern profile (C27-C31) of the consumed plant species with the n-alkanes fecal concentrations of grazing animals, using a non-negative least squares algorithm called EATWHAT software package. The alkane pair C32:C33 and C33 alkane were used to estimate DM intake and diet apparent DM digestibility, respectively. The results showed that daily dry matter intake of the sheep were 1.77, 1.61 and 1.18 kg d-1 in summer, autumn and winter, respectively. Apparent DM digestibility, crude protein (CP), metabolizable energy (ME) and neutral detergent fiber (NDF) intake of diet consumed by sheep decreased significantly (P<0.01) from summer to winter, with no evident changes in ADF and ADL intake. Diet composition indicated Artemisia frigida Willd was the most dominant diet component, contributed 79.68, 68.12 and 86.26% of sheep’s diets in summer, autumn and winter, respectively. Cleistogenes songorica Ohwi and Convolvulus ammannii Desr were the important components of the diet. Although Stipa breviflora Griseb is one of the main plant species in the study area, the sheep rarely choosed it. The study indicated that CP and ME in diet consumed by sheep were deficient in winter. Therefore, appropriate supplementation strategies should be indispensable during this period.

Key words: n-alkanes , diet composition , intake , apparent digestibility , sheep

HU Hong-lian, LIU Yong-zhi, LI Ya-kui, LU De-xun , GAO Min. Use of the N-alkanes to Estimate Intake, Apparent Digestibility and Diet Composition in Sheep Grazing on Stipa breviflora Desert Steppe[J]. Journal of Integrative Agriculture, 2014, 13(5): 1065-1072.

参考文献

Association of Official Analytical Chemists. 1990. Official Methods of Analysis. 15th ed. Arlington, Virginia, USA. Bezabih M, Pellikaan W F, Tolera A, Hendriks W H. 2012. Estimation of feed intake and digestibility in cattle consuming low-quality tropical roughage diets using molasses-based n-alkane boluses. Animal Feed Science and Technology, 177, 161-171

Biligetu. 2003. The influence of stocking rate on plant community and sheep in the grazing system of Stipa breviflora Desert Steppe. MSc thesis, University of Inner Mongolian Agricultural, Huhhot. (in Chinese)

Chopa F S, Nadin L B, Gonda H L. 2012. Two drying methods of bovine faeces for estimating n-alkane concentration, intake and digestibility: A comparison. Animal Feed Science and Technology, 177, 1-6

Ding L M, Long R J. 2010. The use of herbage n-alkanes as markers to estimate the diet composition of yaks on the Qinghai-Tibetan plateau. Asian-Australasian Journal of Animal Sciences, 1, 61-67

Dove H, Mayes R W. 1996. Plant wax components: A new approach to estimating intake and diet composition in herbivores. Journal of Nutrition, 126, 13-26

Dove H, Mayes R W, Freer M. 1996. Effects of species, plant part, and plant age on the n-alkane concentrations in the cuticular wax of pasture plants. Australian Journal of Agricultural Research, 47, 1333-1547

Dove H, Mayes R W. 2005. Using n-alkanes and other plant wax components to estimate intake, digestibility and diet composition of grazing/browsing sheep and goats. Small Ruminant Research, 59, 123-139

Dove H, Mayes R W, Lamb C S, Ellis K J. 2002. Factors influencing the release rate of alkanes from an intra- ruminal, controlled-release device, and the resultant accuracy of intake estimation in sheep. Australian Journal of Agricultural Research, 53, 681-696

Dove H, Moore A D. 1995. Using a least-squares optimization procedure to estimate botanical composition based on the alkanes of plant cuticular wax. Australian Journal of Agricultural Research, 46, 1535-1544

Ferreira L M M, Celaya R, García U, Rodrigues M A M, Osoro K. 2009. Differences between domestic herbivores species in alkane faecal recoveries and the accuracy of subsequent estimates of diet composition. Animal Feed Science and Technology, 151, 128-142

Glindemann T, Wang C, Tas B M, Schiborra A, Gierus M, Taube F, Susenbeth A. 2009. Impact of grazing intensity on herbage intake, composition, and digestibility and on live weight gain of sheep on the Inner Mongolian steppe. Livestock Science, 124, 142-147

Hu H L. 2005. Study on the limiting nutrtional factors of grazing sheep in different grazing periods and optimization supplementary feeding in winter. MSc thesis, University of Inner Mongolian Agricultural, Huhhot. (in Chinese)

Keli A, Andueza D, Vega D A, Guada J A. 2008. Validation of the n-alkane and NIRS techniques to estimate intake, digestibility and diet composition in sheep fed mixed lucerne: Ryegrass diets. Livestock Science, 119, 42-54

Lewis R M, Magadlela A M, Jessop N S, Emmans G C. 2003. The ability of the n-alkane technique to estimate intake and diet choice of sheep. Animal Science, 77, 319- 327.

Liu Y Z. 2005. Study on sheep grazing system of Stipa breviflora Desert Steppe. PhD thesis, University of Inner Mongolian Agricultural, Huhhot. (in Chinese)

Mayes R W, Lamb C S, Colgrove P M. 1986. The use of dosed and herbage n-alkanes as markers for the determination of herbage intake. Journal of Agricultural Science, 107, 161-170

Molina D O, Matamoros I, Pell A N. 2004. Accuracy of estimates of herbage intake of lactating cows using alkanes: Comparison of two types of capsules. Animal Feed Science and Technology, 114, 241-260

Morais J A S, Berchielli T T, Vega A D, Queiroz M F S, Keli A, Reis R A, Bertipaglia L M A, Reis R A. 2011. The validity of n-alkanes to estimate intake and digestibility in Nellore beef cattle fed a tropical grass (Brachiaria brizantha cv. Maraandu). Livestock Science, 135, 184-192

Newman J A, Thompson W A, Penning P D, Mayes R W. 1995. Least squares estimation of diet composition from n-alkanes in herbage and faeces using matrix mathematics. Australian Journal of Agricultural Research, 46, 793-805

NRC. 1981. Nutrient Requirements of Goats. National Academy Press, Washington, D.C. pp. 1-2

Oliván M, Ferreira L M M, Celaya R, Osoro K. 2007. Accuracy of the n-alkane technique for intake estimates in beef cattle using different sampling procedures and feeding levels. Livestock Science, 106, 28-40

Peiretti P G, Meineri G, Miraglia N, Mucciarelli M, Bergero D. 2006. Intake and apparent digesitibility of hay or hay plus concentrate diets determined in horses by the taotal collection of feces and n-alkanes as internal markers. Livestock Science, 100, 189-194

Premaratne S, Fontenot J P, Sharnklin R K. 2005. Use of n-alkane to estimate intake and digestibility by beef steers. Asian-Australasian Journal of Animal Sciences, 18, 1564-1568

Smit H J, Taweel H Z, Tas B M, Tamminga S, Elgersma A. 2005. Comparison of techniques for estimating herbage intake of grazing dairy cows. Journal of Dairy Science, 88, 1827-1836

van Soest P J, Robertson J B, Lewis B A. 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583-3597

Sun Z W, Zhou D W, Ferreira L M M, Zhong Q Z, Lou Y J. 2008. Diet composition, herbage intake and digestibility in Inner Mongolian cashmere goats grazing on native Leymus chinensis plant communities. Livestock Science, 116, 146-155

Tefera S, Mlambo V, Dlamini A M, Koralagama K D N, Mould F L. 2009. Chemical composition and in vitro ruminal fermentation of selected grasses in the semiarid savannas of Swaziland. African Journal of Range and Forage Science, 26, 9-17

Valiente O L, Delgado P, Vega A D , Guada J A.2003. Validation of the n-alkane technique to estimate intake, digestibility, and diet composition in sheep consuming mixed grain: roughage diets. Australian Journal of Agricultural Research, 54, 693-702

Vulich S A, O’Riordan E G, Hanrahan J P. 1991. Use of n-alkanes for the estimation of herbage intake in sheep: Accuracy and precision of the estimates. Journal of Agricultural Science, 116, 319-323

Wang H R, Feng Z C, Lu D X. 1997. Influences of seasonal changes of nutritive values of native pasture on feed intake and performance of grazing sheep. Inner Mongolian Academy of Animal Sciences, (Suppl.), 143- 149. (in Chinese)

Wang X L. 2009. A study on sheep diet of different stocking rates on Stipa breviflora Desert Steppe. MSc thesis, University of Inner Mongolian Agricultural, Huhhot. (in Chinese)

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Use of the N-alkanes to Estimate Intake, Apparent Digestibility and Diet Composition in Sheep Grazing on Stipa breviflora Desert Steppe

Use of the N-alkanes to Estimate Intake, Apparent Digestibility and Diet Composition in Sheep Grazing on Stipa breviflora Desert Steppe

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