Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (12): 2741-2749.DOI: 10.1016/S2095-3119(14)60912-6

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The Establishment of Double-Transgenic Mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo

 BAI Li-jing, JU Hui-ming, MU Yu-lian, YANG Shu-lin, REN Hong-yan, AO Hong, WANG, Chu-duan , LI Kui   

  1. 1、State Key Laboratory for Animal Nutrition, Ministry of Agriculture/Key Laboratory of Farm Animal Genetic Resources and Germplasm
    Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
    2、College of Animal Science, China Agricultural University, Beijing 100193, P.R.China
    3、College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, P.R.China
  • 收稿日期:2013-07-05 出版日期:2014-12-01 发布日期:2014-12-10
  • 通讯作者: LI Kui, Tel: +86-10-62813822, E-mail: likui@caas.cn
  • 作者简介:BAI Li-jing, Tel: +86-10-62833312, E-mail: bailijing0730@163.com
  • 基金资助:

    This work was supported by the National Transgenic Breeding Project of China (2011ZX08010-003) and the National Natural Science Foundation of China (31272405, 31101683).

The Establishment of Double-Transgenic Mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo

 BAI Li-jing, JU Hui-ming, MU Yu-lian, YANG Shu-lin, REN Hong-yan, AO Hong, WANG, Chu-duan , LI Kui   

  1. 1、State Key Laboratory for Animal Nutrition, Ministry of Agriculture/Key Laboratory of Farm Animal Genetic Resources and Germplasm
    Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
    2、College of Animal Science, China Agricultural University, Beijing 100193, P.R.China
    3、College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, P.R.China
  • Received:2013-07-05 Online:2014-12-01 Published:2014-12-10
  • Contact: LI Kui, Tel: +86-10-62813822, E-mail: likui@caas.cn
  • About author:BAI Li-jing, Tel: +86-10-62833312, E-mail: bailijing0730@163.com
  • Supported by:

    This work was supported by the National Transgenic Breeding Project of China (2011ZX08010-003) and the National Natural Science Foundation of China (31272405, 31101683).

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摘要： Type A spermatogonial stem cells are the only immortal diploid cells in the postnatal animal that undergo self-renewal through the lifetime of an animal and transmit genes to subsequent generations. In this paper, the generation and characterization of double-transgenic mice co-expressing the Escherichia coli appA gene and human MxA gene generated via the in vivo transfection of type A spermatogonial cells were reported for the first time. The dicistronic expression vector pcDNA-appA-MxA(AMP) and ExGen500 transfection reagent were injected into the testicular tissue of 7-d-old male ICR mice. The mice that underwent testismediated gene transfer were mated with wild-type female mice, and the integration and expression of the foreign genes in the offspring were evaluated. Transgenic mice that co-expressed appA and MxA showed a gene integration rate of 8.89% (16/180). The transgenic mice were environmentally friendly, as the amount of phosphorous remaining in the manure was reduced by as much as 11.1% by the appA gene (P<0.05); these animals also exhibited a strong anti-viral phenotype.

关键词: type A spermatogonial stem cells , dicistronic expression vector , co-expression , transgenic mice

Abstract: Type A spermatogonial stem cells are the only immortal diploid cells in the postnatal animal that undergo self-renewal through the lifetime of an animal and transmit genes to subsequent generations. In this paper, the generation and characterization of double-transgenic mice co-expressing the Escherichia coli appA gene and human MxA gene generated via the in vivo transfection of type A spermatogonial cells were reported for the first time. The dicistronic expression vector pcDNA-appA-MxA(AMP) and ExGen500 transfection reagent were injected into the testicular tissue of 7-d-old male ICR mice. The mice that underwent testismediated gene transfer were mated with wild-type female mice, and the integration and expression of the foreign genes in the offspring were evaluated. Transgenic mice that co-expressed appA and MxA showed a gene integration rate of 8.89% (16/180). The transgenic mice were environmentally friendly, as the amount of phosphorous remaining in the manure was reduced by as much as 11.1% by the appA gene (P<0.05); these animals also exhibited a strong anti-viral phenotype.

Key words: type A spermatogonial stem cells , dicistronic expression vector , co-expression , transgenic mice