Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (12): 2594-2607.DOI: 10.1016/S2095-3119(14)60756-5

• 论文 • 上一篇    下一篇

Comparative Analysis of Gene Expression Profiling Between Resistant and Susceptible Varieties Infected With Soybean Cyst Nematode Race 4 in Glycine max

 LI Bin, SUN Jun-ming, WANG Lan, ZHAO Rong-juan , WANG Lian-zheng   

  1. The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Laboratory of Soybean Biology (Beijing), MOA/ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
  • 收稿日期:2013-11-19 出版日期:2014-12-01 发布日期:2014-12-10
  • 通讯作者: WANG Lian-zheng, Tel: +86-10-8210-3007, Fax: +86-10-82105805, E-mail: wanglianzheng@caas.cn
  • 作者简介:LI Bin, E-mail: libin02@caas.cn,These authors contributed equally to this work.
  • 基金资助:

    This work was supported by the National Nature Science Foundation of China (31301345 and 31171576), the CAAS Innovation Project, the Genetically Modified Organisms Breeding Major Projects, China (2009ZX08004-003B and 2011ZX08004-003), and the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD35B06-3).

Comparative Analysis of Gene Expression Profiling Between Resistant and Susceptible Varieties Infected With Soybean Cyst Nematode Race 4 in Glycine max

 LI Bin, SUN Jun-ming, WANG Lan, ZHAO Rong-juan , WANG Lian-zheng   

  1. The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Laboratory of Soybean Biology (Beijing), MOA/ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
  • Received:2013-11-19 Online:2014-12-01 Published:2014-12-10
  • Contact: WANG Lian-zheng, Tel: +86-10-8210-3007, Fax: +86-10-82105805, E-mail: wanglianzheng@caas.cn
  • About author:LI Bin, E-mail: libin02@caas.cn,These authors contributed equally to this work.
  • Supported by:

    This work was supported by the National Nature Science Foundation of China (31301345 and 31171576), the CAAS Innovation Project, the Genetically Modified Organisms Breeding Major Projects, China (2009ZX08004-003B and 2011ZX08004-003), and the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD35B06-3).

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摘要： Soybean cyst nematode (SCN) is one of the most devastating pathogen for soybean. Therefore, identification of resistant germplasm resources and resistant genes is needed to improve SCN resistance for soybean. Soybean varieties Huipizhiheidou and Wuzhaiheidou were distributed in China and exhibited broad spectrums of resistance to various SCN races. In this study, these two resistant varieties, combined with standard susceptible varieties (Lee and Essex), were utilized to identify the differentially expressed transcripts after infection with SCN race 4 between resistant and susceptible reactions by using the Affymetrix Soybean Genome GeneChip. Comparative analyses indicated that 21 common genes changed significantly in the resistant group, of which 16 increased and 5 decreased. However, 12 common genes changed significantly in the susceptible group, of which 9 increased and 3 decreased. Additionally, 27 genes were found in common between resistant and susceptible reactions. The 21 significantly changed genes in resistant reaction were associated with disease and defense, cell structure, transcription, metabolism, and signal transduction. The fold induction of 4 from the 21 genes was confirmed by quantitative RT-PCR (qRTPCR) analysis. Moreover, the gene ontology (GO) enrichment analyses demonstrated the serine family amino acid metabolic process and arginine metabolic process may play important roles in SCN resistance. This study provided a new insight on the genetic basis of soybean resistance to SCN race 4, and the identified resistant or resistant-related genes are potentially useful for SCN-resistance breeding in soybean.

关键词: soybean , soybean cyst nematode , Affymetrix Soybean Genome GeneChip , gene ontology

Abstract: Soybean cyst nematode (SCN) is one of the most devastating pathogen for soybean. Therefore, identification of resistant germplasm resources and resistant genes is needed to improve SCN resistance for soybean. Soybean varieties Huipizhiheidou and Wuzhaiheidou were distributed in China and exhibited broad spectrums of resistance to various SCN races. In this study, these two resistant varieties, combined with standard susceptible varieties (Lee and Essex), were utilized to identify the differentially expressed transcripts after infection with SCN race 4 between resistant and susceptible reactions by using the Affymetrix Soybean Genome GeneChip. Comparative analyses indicated that 21 common genes changed significantly in the resistant group, of which 16 increased and 5 decreased. However, 12 common genes changed significantly in the susceptible group, of which 9 increased and 3 decreased. Additionally, 27 genes were found in common between resistant and susceptible reactions. The 21 significantly changed genes in resistant reaction were associated with disease and defense, cell structure, transcription, metabolism, and signal transduction. The fold induction of 4 from the 21 genes was confirmed by quantitative RT-PCR (qRTPCR) analysis. Moreover, the gene ontology (GO) enrichment analyses demonstrated the serine family amino acid metabolic process and arginine metabolic process may play important roles in SCN resistance. This study provided a new insight on the genetic basis of soybean resistance to SCN race 4, and the identified resistant or resistant-related genes are potentially useful for SCN-resistance breeding in soybean.

Key words: soybean , soybean cyst nematode , Affymetrix Soybean Genome GeneChip , gene ontology