Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine

doi:10.1016/S2095-3119(13)60432-3

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (7): 1152-1161.DOI: 10.1016/S2095-3119(13)60432-3

Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine

ZHANG Yi-ming, NI Xi-lu, MA Hui-qin , Wenping Qiu

1.Department of Fruit Tree Sciences, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P. R. China
2.The Center for Grapevine Biotechnology, William H. Darr School of Agriculture, Missouri State University, Mountain Grove, MO 65711,USA
3. Ningxia Forestry Institute, State Key Laboratory of Seedling Bioengineering, Yinchuan 750004, P.R.China
4.College of Life Science, Northwest University, Xi’an 710069, P.R.China

收稿日期:2012-09-07 出版日期:2013-07-01 发布日期:2013-07-04
通讯作者: Correspondence MA Hui-qin, E-mail: hqma@cau.edu.cn; Wenping Qiu, E-mail: wenpingqiu@missouristate.edu
作者简介:ZHANG Yi-ming, Mobile: 15041248655, E-mail: zhangyiming522@gmail.com
基金资助:
This project was supported by a grant from the United States Department of Agriculture (USDA-CSREES 2009- 38901-19962) and a scholarship by the China Scholarship Foundation Council.

Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine

ZHANG Yi-ming, NI Xi-lu, MA Hui-qin , Wenping Qiu

1.Department of Fruit Tree Sciences, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P. R. China
2.The Center for Grapevine Biotechnology, William H. Darr School of Agriculture, Missouri State University, Mountain Grove, MO 65711,USA
3. Ningxia Forestry Institute, State Key Laboratory of Seedling Bioengineering, Yinchuan 750004, P.R.China
4.College of Life Science, Northwest University, Xi’an 710069, P.R.China

Received:2012-09-07 Online:2013-07-01 Published:2013-07-04
Contact: Correspondence MA Hui-qin, E-mail: hqma@cau.edu.cn; Wenping Qiu, E-mail: wenpingqiu@missouristate.edu
About author:ZHANG Yi-ming, Mobile: 15041248655, E-mail: zhangyiming522@gmail.com
Supported by:
This project was supported by a grant from the United States Department of Agriculture (USDA-CSREES 2009- 38901-19962) and a scholarship by the China Scholarship Foundation Council.

摘要/Abstract

摘要： Non-expressor of pathogenesis-related genes 1 (NPR1) plays a significant role in the defense responses of plants to pathogens by regulating the expression of defense-related genes. In the present study, we isolated two NPR1 genes from Vitis aestivalis cv. Norton and Vitis vinifera cv. Cabernet Sauvignon, which were referred to as VaNPR1.1 and VvNPR1. 1-CS, respectively. They encode a protein of 584 amino acids with a predicted molecular weight of 64.8 kDa and a theoretical isoelectric point (pI) of 5.74. The predicted amino acid sequences of VaNPR1.1 and VvNPR1.1-CS differ by only one amino acid. Over-expression of VaNPR1.1 gene in Arabidopsis npr1-1 mutant plants restores the transcriptional expression of AtPR-1 gene, though not to the full scale. This result demonstrated that a grapevine VaNPR1.1 possesses a similar function to the Arabidopsis NPR1 in the regulation of defense-related genes. Over-expression of VaNPR1.1 in transgenic Arabidopsis plant increased tolerance to salinity, but had no effect on the drought tolerance. We conclude that VaNPR1.1 is a functional ortholog of AtNPR1 and also involved in grapevine’s response to the salt stress.

关键词: NPR1 , pathogenesis-related genee , over-expression , transgenic Arabidopsis

Abstract: Non-expressor of pathogenesis-related genes 1 (NPR1) plays a significant role in the defense responses of plants to pathogens by regulating the expression of defense-related genes. In the present study, we isolated two NPR1 genes from Vitis aestivalis cv. Norton and Vitis vinifera cv. Cabernet Sauvignon, which were referred to as VaNPR1.1 and VvNPR1. 1-CS, respectively. They encode a protein of 584 amino acids with a predicted molecular weight of 64.8 kDa and a theoretical isoelectric point (pI) of 5.74. The predicted amino acid sequences of VaNPR1.1 and VvNPR1.1-CS differ by only one amino acid. Over-expression of VaNPR1.1 gene in Arabidopsis npr1-1 mutant plants restores the transcriptional expression of AtPR-1 gene, though not to the full scale. This result demonstrated that a grapevine VaNPR1.1 possesses a similar function to the Arabidopsis NPR1 in the regulation of defense-related genes. Over-expression of VaNPR1.1 in transgenic Arabidopsis plant increased tolerance to salinity, but had no effect on the drought tolerance. We conclude that VaNPR1.1 is a functional ortholog of AtNPR1 and also involved in grapevine’s response to the salt stress.

Key words: NPR1 , pathogenesis-related genee , over-expression , transgenic Arabidopsis

ZHANG Yi-ming, NI Xi-lu, MA Hui-qin , Wenping Qiu. Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine[J]. Journal of Integrative Agriculture, 2013, 12(7): 1152-1161.

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Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine

Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine

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