Bzw2 Promotes Proliferation and Lactation of Mammary Epithelial Cell in Dairy Goat

doi:10.1016/S1671-2927(00)8724

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (11): 1884-1891.DOI: 10.1016/S1671-2927(00)8724

Bzw2 Promotes Proliferation and Lactation of Mammary Epithelial Cell in Dairy Goat

SUN Rui-qiu, LI Qing-zhang, YAN Hong-bo, ZHAO Jing, GAO Xue-jun

Key Laboratory of Dairy Science, Ministry of Education/College of Life Science, Northeast Agricultural University, Harbin 150030, P.R. China

收稿日期:2011-07-05 出版日期:2012-11-01 发布日期:2012-11-24
通讯作者: Correspondence LI Qing-zhang, E-mail: qingzhangli@hotmail.com
作者简介:SUN Rui-qiu, Mobile: 13512968651, E-mail: sunruiqiu919@hotmail.com
基金资助:
This work was supported by the International Cooperation Project of Heilongjiang Province, China (WB07A06)and Innovation Team Project of Northeast Agricultural University, China (CXT005-1-1/-2).

Bzw2 Promotes Proliferation and Lactation of Mammary Epithelial Cell in Dairy Goat

SUN Rui-qiu, LI Qing-zhang, YAN Hong-bo, ZHAO Jing, GAO Xue-jun

Key Laboratory of Dairy Science, Ministry of Education/College of Life Science, Northeast Agricultural University, Harbin 150030, P.R. China

Received:2011-07-05 Online:2012-11-01 Published:2012-11-24
Contact: Correspondence LI Qing-zhang, E-mail: qingzhangli@hotmail.com
About author:SUN Rui-qiu, Mobile: 13512968651, E-mail: sunruiqiu919@hotmail.com
Supported by:
This work was supported by the International Cooperation Project of Heilongjiang Province, China (WB07A06)and Innovation Team Project of Northeast Agricultural University, China (CXT005-1-1/-2).

摘要/Abstract

摘要： Mitosis of mammary epithelial cell is foundation of mammal lactation. We developed a strategy of combined application of generation of longer cDNA fragments from the serial analysis of gene expression (SAGE) tags for gene identification (GLGI) to screen and identify genes influencing lactating ability of mammary epithelial cell in dairy goat. GLGI as a new tag identification technique was brought about with SAGE. Bzw2 was found as a candidate gene related to lactation by screening Long-SAGE library of mammary gland in dairy goat. Bzw2 cDNA was synthesized by switching mechanism at 5´-end of RNA transcript (SMART) technology. The mRNA level of Bzw2 was relatively higher in early lactation than in other development stages of mammary gland. The proliferation of mammary epithelial cell was inhibited by transfecting specific shRNA of Bzw2. The mRNA levels of Stat5, Csn2 and Prlr were also down-regulated, suggesting the lactating ability of mammary epithelial cell was attenuated after Bzw2 RNAi. The reduction of mammary epithelial cell growth and lactation by Bzw2 RNAi was rescued through over-expression of Bzw2. These results revealed that Bzw2 might play an important role in lactation though the molecular mechanism was still unclear.

关键词: Bzw2 , mammary epithelial cells , proliferation , lactation , RNAi , over-expression

Abstract: Mitosis of mammary epithelial cell is foundation of mammal lactation. We developed a strategy of combined application of generation of longer cDNA fragments from the serial analysis of gene expression (SAGE) tags for gene identification (GLGI) to screen and identify genes influencing lactating ability of mammary epithelial cell in dairy goat. GLGI as a new tag identification technique was brought about with SAGE. Bzw2 was found as a candidate gene related to lactation by screening Long-SAGE library of mammary gland in dairy goat. Bzw2 cDNA was synthesized by switching mechanism at 5´-end of RNA transcript (SMART) technology. The mRNA level of Bzw2 was relatively higher in early lactation than in other development stages of mammary gland. The proliferation of mammary epithelial cell was inhibited by transfecting specific shRNA of Bzw2. The mRNA levels of Stat5, Csn2 and Prlr were also down-regulated, suggesting the lactating ability of mammary epithelial cell was attenuated after Bzw2 RNAi. The reduction of mammary epithelial cell growth and lactation by Bzw2 RNAi was rescued through over-expression of Bzw2. These results revealed that Bzw2 might play an important role in lactation though the molecular mechanism was still unclear.

Key words: Bzw2 , mammary epithelial cells , proliferation , lactation , RNAi , over-expression

SUN Rui-qiu, LI Qing-zhang, YAN Hong-bo, ZHAO Jing, GAO Xue-jun. Bzw2 Promotes Proliferation and Lactation of Mammary Epithelial Cell in Dairy Goat[J]. Journal of Integrative Agriculture, 2012, 12(11): 1884-1891.

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Bzw2 Promotes Proliferation and Lactation of Mammary Epithelial Cell in Dairy Goat

Bzw2 Promotes Proliferation and Lactation of Mammary Epithelial Cell in Dairy Goat

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