Exogenous Hydrogen Sulfide Enhanced Antioxidant Capacity, Amylase Activities and Salt Tolerance of Cucumber Hypocotyls and Radicles

doi:10.1016/S2095-3119(13)60245-2

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (3): 445-456.DOI: 10.1016/S2095-3119(13)60245-2

Exogenous Hydrogen Sulfide Enhanced Antioxidant Capacity, Amylase Activities and Salt Tolerance of Cucumber Hypocotyls and Radicles

YU Li-xu, ZHANG Cun-jia, SHANG Hong-qin, WANG Xiu-feng, WEI Min, YANG Feng-juan , SHIQing-hua

1.State Key Laboratory of Crop Biology/College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China
2.Department of Life Sciences, Heze University, Heze 274015, P.R.China

收稿日期:2012-08-22 出版日期:2013-03-01 发布日期:2013-03-10
通讯作者: Correspondence SHI Qing-hua, Tel: +86-538-8242201, E-mail: qhshi@sdau.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (30800751) and the National Basic Research Program of China (2009CB119000).

Exogenous Hydrogen Sulfide Enhanced Antioxidant Capacity, Amylase Activities and Salt Tolerance of Cucumber Hypocotyls and Radicles

YU Li-xu, ZHANG Cun-jia, SHANG Hong-qin, WANG Xiu-feng, WEI Min, YANG Feng-juan , SHIQing-hua

1.State Key Laboratory of Crop Biology/College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China
2.Department of Life Sciences, Heze University, Heze 274015, P.R.China

Received:2012-08-22 Online:2013-03-01 Published:2013-03-10
Contact: Correspondence SHI Qing-hua, Tel: +86-538-8242201, E-mail: qhshi@sdau.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (30800751) and the National Basic Research Program of China (2009CB119000).

摘要/Abstract

摘要： In the present experiment, effects of sodium hydrosulfide (NaHS), a H2S donor, on the oxidative damage, antioxidant capacity and the growth of cucumber hypocotyls and radicles were studied under 100 mmol L-1 NaCl stress. NaCl treatment significantly induced accumulation of H2O2 and thiobarbituric acid-reactive substances (TBARS) in cucumber hypocotyls and radicles, and application of NaHS dramatically reduced the accumulation of H2O2 and lipid peroxidation. However, the alleviating effects greatly depended on the concentrations of NaHS, and 400 μmol L-1 NaHS treatment showed the most significant effects. Corresponding to the change of lipid peroxidation, higher activities of antioxidant enzymes as well as the antioxidant capacity indicated as DPPH scavenging activity, chelating activity of ferrous ions and hydroxyl radical (·OH) scavenging activity were induced by NaHS treatment under NaCl stress, especially by 400 μmol L-1 NaHS treatment. With the alleviating lipid peroxidation, the amylase activities in cotyledons were increased, and the length of cucumber hypocotyls and radicles were significantly promoted by NaHS treatment under NaCl stress. Unlike the effects of NaHS, pretreatment with other sodium salts including Na2S, Na2SO4, NaHSO4, Na2SO3, NaHSO3 and NaAc did not show significant effects on the growth of cucumber hypocotyls and radicles. These salts do not release H2S. Based on above results, it can be concluded that the effects of NaHS in the experiment depended on the H2S rather than other compounds derived from NaHS, and the alleviating effects might related with its function in modulating antioxidant capacity and amylase activities.

关键词: cucumber, tolerance, salt stress, seed priming, hydrogen sulfide, antioxidant capacity

Abstract: In the present experiment, effects of sodium hydrosulfide (NaHS), a H2S donor, on the oxidative damage, antioxidant capacity and the growth of cucumber hypocotyls and radicles were studied under 100 mmol L-1 NaCl stress. NaCl treatment significantly induced accumulation of H2O2 and thiobarbituric acid-reactive substances (TBARS) in cucumber hypocotyls and radicles, and application of NaHS dramatically reduced the accumulation of H2O2 and lipid peroxidation. However, the alleviating effects greatly depended on the concentrations of NaHS, and 400 μmol L-1 NaHS treatment showed the most significant effects. Corresponding to the change of lipid peroxidation, higher activities of antioxidant enzymes as well as the antioxidant capacity indicated as DPPH scavenging activity, chelating activity of ferrous ions and hydroxyl radical (·OH) scavenging activity were induced by NaHS treatment under NaCl stress, especially by 400 μmol L-1 NaHS treatment. With the alleviating lipid peroxidation, the amylase activities in cotyledons were increased, and the length of cucumber hypocotyls and radicles were significantly promoted by NaHS treatment under NaCl stress. Unlike the effects of NaHS, pretreatment with other sodium salts including Na2S, Na2SO4, NaHSO4, Na2SO3, NaHSO3 and NaAc did not show significant effects on the growth of cucumber hypocotyls and radicles. These salts do not release H2S. Based on above results, it can be concluded that the effects of NaHS in the experiment depended on the H2S rather than other compounds derived from NaHS, and the alleviating effects might related with its function in modulating antioxidant capacity and amylase activities.

YU Li-xu, ZHANG Cun-jia, SHANG Hong-qin, WANG Xiu-feng, WEI Min, YANG Feng-juan , SHIQing-hua. Exogenous Hydrogen Sulfide Enhanced Antioxidant Capacity, Amylase Activities and Salt Tolerance of Cucumber Hypocotyls and Radicles[J]. Journal of Integrative Agriculture, 2013, 12(3): 445-456.

YU Li-xu, ZHANG Cun-jia, SHANG Hong-qin, WANG Xiu-feng, WEI Min, YANG Feng-juan , SHIQing-hua . Exogenous Hydrogen Sulfide Enhanced Antioxidant Capacity, Amylase Activities and Salt Tolerance of Cucumber Hypocotyls and Radicles[J]. Journal of Integrative Agriculture, 2013, 12(3): 445-456.

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Exogenous Hydrogen Sulfide Enhanced Antioxidant Capacity, Amylase Activities and Salt Tolerance of Cucumber Hypocotyls and Radicles

Exogenous Hydrogen Sulfide Enhanced Antioxidant Capacity, Amylase Activities and Salt Tolerance of Cucumber Hypocotyls and Radicles

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