Relationships Between C4 Enzyme Activities and Yield in Soybeans (Glycine max (L.) Merr.)

doi:10.1016/S2095-3119(13)60240-3

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (3): 406-413.DOI: 10.1016/S2095-3119(13)60240-3

Relationships Between C4 Enzyme Activities and Yield in Soybeans (Glycine max (L.) Merr.)

HUANG Shan-shan, LI Chang-suo, YANG Ming-liang, LI Wen-bin , WANG Ji-an

1.Key Laboratory of Soybean Biology, Ministry of Education/Soybean Research Institute, Northeast Agricultural University, Harbin 150030, P.R.China
2.Plant Protection Institute, Heilongjiang Academy of Land Reclamation Sciences, Harbin 150038, P.R.China

出版日期:2013-03-01 发布日期:2013-03-10
通讯作者: Correspondence WANG Ji-an, Tel: +86-451-55190692, E-mail: wangsoy@yahoo.com.cn
作者简介:HUANG Shan-shan, E-mail: huangshanshan.6@163.com
基金资助:
This study was supported by the National Natural Science Foundation of China (30471092) and the Genetically Modified Organisms Breeding Major Projects, China (2009ZX08009- 089B).

Relationships Between C4 Enzyme Activities and Yield in Soybeans (Glycine max (L.) Merr.)

HUANG Shan-shan, LI Chang-suo, YANG Ming-liang, LI Wen-bin , WANG Ji-an

1.Key Laboratory of Soybean Biology, Ministry of Education/Soybean Research Institute, Northeast Agricultural University, Harbin 150030, P.R.China
2.Plant Protection Institute, Heilongjiang Academy of Land Reclamation Sciences, Harbin 150038, P.R.China

Online:2013-03-01 Published:2013-03-10
Contact: Correspondence WANG Ji-an, Tel: +86-451-55190692, E-mail: wangsoy@yahoo.com.cn
About author:HUANG Shan-shan, E-mail: huangshanshan.6@163.com
Supported by:
This study was supported by the National Natural Science Foundation of China (30471092) and the Genetically Modified Organisms Breeding Major Projects, China (2009ZX08009- 089B).

摘要/Abstract

摘要： To study the relationships between C4 enzyme activities and yield, C4 enzyme activities (phosphoenolpyruvate carboxylase (PEPCase), NADP-malate dehydrogenase (NADP-MDH), NADP-malic enzyme (NADP-ME), and pyruvate phosphate dikinase (PPDK)) in different organs of ten soybean cultivars with different yields were measured at different growth stages in China. The result showed that four enzyme activities in C4 pathway were obviously different among cultivars, especially PPDK activity was not detected in the leaves of Dongnong 1567 and Dongnong 1068 and the young leaves of Gongjiao 9107-1 and Dongnong 97-172, but there were weak activities in pod coats. The order of C4 enzyme activities is young leaves < old leaves < pod coats. The correlation coefficients between PEPCase activity and yield and between NADP-MDH activity at blooming stage and yield were 0.6979 and 0.6565, respectively, and both reached the significant level (5%), and PEPCase activity kept significant positive correlation with plant photosynthetic rate. There was a negative correlation between NADP-ME activity and yield, and no correlation was found between PPDK activity and yield.

关键词: soybean , photosynthetic rate , yield , C4 enzyme

Abstract: To study the relationships between C4 enzyme activities and yield, C4 enzyme activities (phosphoenolpyruvate carboxylase (PEPCase), NADP-malate dehydrogenase (NADP-MDH), NADP-malic enzyme (NADP-ME), and pyruvate phosphate dikinase (PPDK)) in different organs of ten soybean cultivars with different yields were measured at different growth stages in China. The result showed that four enzyme activities in C4 pathway were obviously different among cultivars, especially PPDK activity was not detected in the leaves of Dongnong 1567 and Dongnong 1068 and the young leaves of Gongjiao 9107-1 and Dongnong 97-172, but there were weak activities in pod coats. The order of C4 enzyme activities is young leaves < old leaves < pod coats. The correlation coefficients between PEPCase activity and yield and between NADP-MDH activity at blooming stage and yield were 0.6979 and 0.6565, respectively, and both reached the significant level (5%), and PEPCase activity kept significant positive correlation with plant photosynthetic rate. There was a negative correlation between NADP-ME activity and yield, and no correlation was found between PPDK activity and yield.

Key words: soybean , photosynthetic rate , yield , C4 enzyme

HUANG Shan-shan, LI Chang-suo, YANG Ming-liang, LI Wen-bin , WANG Ji-an. Relationships Between C4 Enzyme Activities and Yield in Soybeans (Glycine max (L.) Merr.)[J]. Journal of Integrative Agriculture, 2013, 12(3): 406-413.

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Relationships Between C4 Enzyme Activities and Yield in Soybeans (Glycine max (L.) Merr.)

Relationships Between C4 Enzyme Activities and Yield in Soybeans (Glycine max (L.) Merr.)

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