Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558

doi:10.1016/S2095-3119(13)60239-7

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (3): 398-405.DOI: 10.1016/S2095-3119(13)60239-7

Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558

ZHENG Yong-sheng, MA Xiao-gang, CHI De-zhao, GAO Ai-nong, LI Li-hui , LIU Wei-hua

1.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Qinghai Academy of Agriculture and Forestry, Xining 810000, P.R.China

收稿日期:2012-05-02 出版日期:2013-03-01 发布日期:2013-03-10
通讯作者: Correspondance LIU Wei-hua, Tel: +86-10-62176077, Fax: +86-10-62189650, E-mail: liuweihua@caas.cn
作者简介:ZHENG Yong-sheng, Tel: +86-531-83178713, E-mail: zhengyongsheng123@163.com;
基金资助:
This work was supported by the National High Technology Research and Development Program of China (2011AA100102 and 2006AA10Z174).

Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558

ZHENG Yong-sheng, MA Xiao-gang, CHI De-zhao, GAO Ai-nong, LI Li-hui , LIU Wei-hua

1.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Qinghai Academy of Agriculture and Forestry, Xining 810000, P.R.China

Received:2012-05-02 Online:2013-03-01 Published:2013-03-10
Contact: Correspondance LIU Wei-hua, Tel: +86-10-62176077, Fax: +86-10-62189650, E-mail: liuweihua@caas.cn
About author:ZHENG Yong-sheng, Tel: +86-531-83178713, E-mail: zhengyongsheng123@163.com;
Supported by:
This work was supported by the National High Technology Research and Development Program of China (2011AA100102 and 2006AA10Z174).

摘要/Abstract

摘要： Spike number is one of three yield-related factors and is closely related to wheat yield. In the present study, we found that the inhibited and normal tillers of the 3558 line presented phenotypic differences at the elongation stage by morphological and anatomical analysis. We then initiated a proteomic study using two-dimensional electrophoresis (2-DE) and nanoscale liquid chromatography-high-definition tandem mass spectroscopy, to isolate and identify the key proteins and metabolic pathways related to spike-development inhibition. A total of 31 differentially expressed proteins (DEPs), which were mainly involved in cell cycle regulation, photosynthesis, glycolysis, stress response, and oxidation-reduction reactions, were isolated and identified. 14-3-3-like proteins and proliferating cell nuclear antigen (PCNA), involved in cell-cycle regulation, were dramatically down-regulated in inhibited tillers compared to normal tillers. Six spots corresponding to degraded Rubisco large subunits, involved in photosynthesis, were detected in different locations of the 2-DE gels and were up-regulated in inhibited tillers. In addition, the relative levels of DEPs involved in glycolysis and oxidationreduction reactions changed dramatically. Development was blocked or delayed at the elongation stage in the inhibited tillers of 3558. Weakened energy metabolism might be one reason that the inhibited tillers could not joint and develop into spikes. These DEPs and related metabolic pathways are significant for understanding the mechanism of spike-development inhibition and studying the spike-development process in wheat.

关键词: wheat , spike-development inhibition , 2-DE , differentially expressed proteins

Abstract: Spike number is one of three yield-related factors and is closely related to wheat yield. In the present study, we found that the inhibited and normal tillers of the 3558 line presented phenotypic differences at the elongation stage by morphological and anatomical analysis. We then initiated a proteomic study using two-dimensional electrophoresis (2-DE) and nanoscale liquid chromatography-high-definition tandem mass spectroscopy, to isolate and identify the key proteins and metabolic pathways related to spike-development inhibition. A total of 31 differentially expressed proteins (DEPs), which were mainly involved in cell cycle regulation, photosynthesis, glycolysis, stress response, and oxidation-reduction reactions, were isolated and identified. 14-3-3-like proteins and proliferating cell nuclear antigen (PCNA), involved in cell-cycle regulation, were dramatically down-regulated in inhibited tillers compared to normal tillers. Six spots corresponding to degraded Rubisco large subunits, involved in photosynthesis, were detected in different locations of the 2-DE gels and were up-regulated in inhibited tillers. In addition, the relative levels of DEPs involved in glycolysis and oxidationreduction reactions changed dramatically. Development was blocked or delayed at the elongation stage in the inhibited tillers of 3558. Weakened energy metabolism might be one reason that the inhibited tillers could not joint and develop into spikes. These DEPs and related metabolic pathways are significant for understanding the mechanism of spike-development inhibition and studying the spike-development process in wheat.

Key words: wheat , spike-development inhibition , 2-DE , differentially expressed proteins

ZHENG Yong-sheng, MA Xiao-gang, CHI De-zhao, GAO Ai-nong, LI Li-hui , LIU Wei-hua. Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558[J]. Journal of Integrative Agriculture, 2013, 12(3): 398-405.

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Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558

Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558

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