Hoxc13/β-catenin Correlation with Hair Follicle Activity in Cashmere Goat

doi:10.1016/S1671-2927(00)8642

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (7): 1159-1166.DOI: 10.1016/S1671-2927(00)8642

Hoxc13/β-catenin Correlation with Hair Follicle Activity in Cashmere Goat

WU Jiang-hong, ZHANG Yan-jun, ZHANG Jia-xin, CHANG Zi-li, LI Jin-quan, YAN Zu-wei, Husile , ZHANG Wen-guang

1.Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolian Autonomous Region, Hohhot 010018, P.R.China
2.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, P.R.China
3.College of Science, Inner Mongolia Agricultural University, Huhhot 010018, P.R.China

收稿日期:2011-01-19 出版日期:2012-07-01 发布日期:2012-07-27
通讯作者: ZHANG Wen-guang, E-mail: atcgnmbi@yahoo.com.cn; LI Jin-quan, Mobile: 15047996076,E-mail: lijinquan_nd@126.com
作者简介:WU Jiang-hong, E-mail: wujianghonglong@126.com
基金资助:
This research was conducted with financial support from the National Natural Science Foundation of China (30960246), the Key Project of National Science and Technology Pillar Program of China (2011BAD28B05), the National High Technology Research and Development Program of China (2007AA10Z151), the Specialized Research Fund for the Doctoral Program of Higher Education (20091515120010), the Inner Mongolia Natural Science Foundation, China (20080404ZD04), and the China Agriculture Research System (CARS-40).

Hoxc13/β-catenin Correlation with Hair Follicle Activity in Cashmere Goat

WU Jiang-hong, ZHANG Yan-jun, ZHANG Jia-xin, CHANG Zi-li, LI Jin-quan, YAN Zu-wei, Husile , ZHANG Wen-guang

1.Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolian Autonomous Region, Hohhot 010018, P.R.China
2.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, P.R.China
3.College of Science, Inner Mongolia Agricultural University, Huhhot 010018, P.R.China

Received:2011-01-19 Online:2012-07-01 Published:2012-07-27
Contact: ZHANG Wen-guang, E-mail: atcgnmbi@yahoo.com.cn; LI Jin-quan, Mobile: 15047996076,E-mail: lijinquan_nd@126.com
About author:WU Jiang-hong, E-mail: wujianghonglong@126.com
Supported by:
This research was conducted with financial support from the National Natural Science Foundation of China (30960246), the Key Project of National Science and Technology Pillar Program of China (2011BAD28B05), the National High Technology Research and Development Program of China (2007AA10Z151), the Specialized Research Fund for the Doctoral Program of Higher Education (20091515120010), the Inner Mongolia Natural Science Foundation, China (20080404ZD04), and the China Agriculture Research System (CARS-40).

摘要/Abstract

摘要： Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats (Caprus hircus) is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes (HFDRGs) in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxc13 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFPHoxc13. The result suggested that overexpression of Hoxc13 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression.

关键词: Hoxc13, &beta, -catenin, Cashmere goat, hair follicle activity, cycle

Abstract: Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats (Caprus hircus) is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes (HFDRGs) in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxc13 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFPHoxc13. The result suggested that overexpression of Hoxc13 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression.

Key words: Hoxc13, β-catenin, Cashmere goat, hair follicle activity, cycle

WU Jiang-hong, ZHANG Yan-jun, ZHANG Jia-xin, CHANG Zi-li, LI Jin-quan, YAN Zu-wei, Husile , ZHANG Wen-guang . Hoxc13/β-catenin Correlation with Hair Follicle Activity in Cashmere Goat[J]. Journal of Integrative Agriculture, 2012, 12(7): 1159-1166.

参考文献

[1]Awgulewitsch A. 2003. Hox in hair growth and development. Naturwissenschaften, 90, 193-211.

[2]Fischer T W, Slominski A, Tobin D J, Paus R. 2008. Melatonin and the hair follicle. Journal of Pineal Research, 44, 1-15.

[3]Fuchs E. 2007. Scratching the surface of skin development. Nature, 445, 834-842.

[4]Huelsken J, Vogel R, Erdmann B, Cotsarelis G, Birchmeier W. 2001. β-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin. Cell, 105, 533-545.

[5]Jamora C, Lee P, Kocieniewski P, Azhar M, Hosokawa R, Chai Y, Fuchs E. 2005. A signaling pathway involving TGF-β2 and snail in hair follicle morphogenesis. PLoS Biology, 3, e11. Jave-Suarez L F, Schweizer J, 2006. The HOXC13-controlled expression of early hair keratin genes in the human hair follicle does not involve TALE proteins MEIS and PREP as cofactors. Archives of Dermatological Research, 297, 372-376.

[6]Jia Z H, The T H, An M. 1996. Effects of exogenous melatonin on the productivity of cashmere goats. In: VI International Conference on Goats. International Academic Publishers, China. pp. 890-893.

[7]Jin W, Kim B C, Tognon C, Lee H J, Patel S, Lannon C L, Maris J M, Triche T J, Sorensen P H, Kim S J. 2005. The ETV6-NTRK3 chimeric tyrosine kinase suppresses TGF-β signaling by inactivating the TGF-β type II receptor. Proceedings of the National Academy of Sciences of the United States of America, 102, 16239-16244.

[8]Lo Celso C, Prowse D M, Watt F M. 2004. Transient activation of β-catenin signalling in adult mouse epidermis is sufficient to induce new hair follicles but continuous activation is required to maintain hair follicle tumours. Development, 131, 1787-1799.

[9]Narhi K, Jarvinen E, Birchmeier W, Taketo M M, Mikkola M L, Thesleff I. 2008. Sustained epithelial β-catenin activity induces precocious hair development but disrupts hair follicle down-growth and hair shaft formation. Development, 135, 1019-1028.

[10]Nixon A J. 1993. A method for determining the activity state of hair follicles. Biotechnic and Histochemistry, 68, 316-325.

[11]Nixon A J, Choy V J, Parry A L, Pearson A J. 1993. Fiber growth initiation in hair follicles of goats treated with melatonin. Journal of Experimental Zoology, 267, 47-56.

[12]Ouji Y, Yoshikawa M, Shiroi A, Ishizaka S. 2006. Promotion of hair follicle development and trichogenesis by Wnt-10b in cultured embryonic skin and in reconstituted skin. Biochemical and Biophysical Research Communications, 345, 581-587.

[13]Plikus M V, Mayer J A, de la Cruz D, Baker R E, Maini P K, Maxson R, Chuong C M. 2008. Cyclic dermal BMP signalling regulates stem cell activation during hair regeneration. Nature, 451, 340-344.

[14]Pruett N D, Tkatchenko T V, Jave-Suarez L, Jacobs D F, Potter C S, Tkatchenko A V, Schweizer J, Awgulewitsch A. 2004. Krtap16, characterization of a new hair keratinassociated protein (KAP) gene complex on mouse chromosome 16 and evidence for regulation by Hoxc13. Journal of Biological Chemistry, 279, 51524-51533.

[15]Sander G R, Powell B C. 2004. Structure and expression of the ovine Hoxc-13 gene. Gene, 327, 107-116.

[16]Schmittgen T D, Livak K J. 2008. Analyzing real-time PCR data by the comparative C(T) method. Nature Protocols, 3, 1101-1108.

[17]Sohn K C, Shi G, Jang S, Choi D K, Lee Y, Yoon T J, Park H, Hwang C, Kim H J, Seo Y J, et al. 2009. Pitx2, a β-cateninregulated transcription factor, regulates the differentiation of outer root sheath cells cultured in vitro. Journal of Dermatological Science, 54, 6-11.

[18]Su R, Zhang W G, Sharma R, Chang Z L, Yin J, Li J Q. 2009. Characterization of BMP2 gene expression in embryonic and adult Inner mongolia cashmere goat (Capra hircus) hair follicles. Canadian Journal of Animal Science, 89, 457-462.

[19]Teh T H, Jia Z H, Odgen K D, Newtona G I. 1991. The effects of photoperiod and melatonin implant on cashmere production. Journal of Animal Science, 69, 496.

[20]Wang L, Lu D, Sun H, Zhao X, Shan D, Yang G, Fang L, 2007. Effects of photoperiod and melatonin on nitrogen partitioning and production performance of Inner mongolia white cashmere goats. Frontiers of Agriculture in China, 1, 229-236.

[21]Wang W P, Widelitz R B, Jiang T X, Chuong C M. 1999. Msx-2 and the regulation of organ size: epidermal thickness and hair length. Journal of Investigative Dermatology Symposium Proceedings, 4, 278-281.

[22]Wu J H, Yan Z W, Husile, Zhang W G, Li J Q. 2010. Hoxc13 and the development of hair follicle. Hereditas, 32, 656-662. (in Chinese)

[23]Wu J H, Zhang W G, Li J G, Yin J, Zhang Y J. 2009. Hoxc13 expression pattern in cashmere goat skin during hair follicle development. Agricultural Sciences in China, 8, 491-496.

[24]Wuliji T, Litherland A, Goetsch A L, Sahlu T, Puchala R, Dawson L J, Gipson T. 2006. Evaluation of melatonin and bromocryptine administration in Spanish goats: III. Effects on hair follicle activity, density and relationships between follicle characteristics. Small Ruminant Research, 66, 11-21.

[25]Yin J. 2004. The research of hair follicle development, growth cycle and related genes in Inner Mongolia cashmere goat. Ph D thesis, Inner Mongolia University, Hohhot. (in Chinese)

[26]Zhang Y, Andl T, Yang S H, Teta M, Liu F, Seykora J T, Tobias J W, Piccolo S, Schmidt-Ullrich R, Nagy A, et al. 2008. Activation of β-catenin signaling programs embryonic epidermis to hair follicle fate. Development 135, 2161-2172.

Hoxc13/β-catenin Correlation with Hair Follicle Activity in Cashmere Goat

Hoxc13/β-catenin Correlation with Hair Follicle Activity in Cashmere Goat

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