Effects of dietary yeast β-glucan on nutrient digestibility and serum profiles in pre-ruminant Holstein calves

doi:10.1016/S2095-3119(14)60843-1

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (4): 749-757.DOI: 10.1016/S2095-3119(14)60843-1

Effects of dietary yeast β-glucan on nutrient digestibility and serum profiles in pre-ruminant Holstein calves

MA Tao, TU Yan, ZHANG Nai-feng, GUO Jiang-peng, DENG Kai-dong, ZHOU Yi, YUN Qiang, DIAO Qi-yu

1、Feed Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology, Ministry ofAgriculture, Beijing 100081, P.R.China
2、Beijing General Station of Animal Husbandry, Beijing 100081, P.R.China

收稿日期:2014-03-14 出版日期:2015-04-01 发布日期:2015-04-10
通讯作者: DIAO Qi-yu, Tel: +86-10-82106055, E-mail: diaoqiyu@caas.cn
作者简介:MA Tao, E-mail: matao0229@gmail.com;* These authors contributed equally to this study.
基金资助:
This study was supported by the Earmarked Fund for Beijing Dairy Industry Innovation Team, the Beijing Key Technology for Early Weaning of Calves, and the National Key Technology R&D Program of China (2012BAD12B06).

Effects of dietary yeast β-glucan on nutrient digestibility and serum profiles in pre-ruminant Holstein calves

MA Tao, TU Yan, ZHANG Nai-feng, GUO Jiang-peng, DENG Kai-dong, ZHOU Yi, YUN Qiang, DIAO Qi-yu

1、Feed Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology, Ministry ofAgriculture, Beijing 100081, P.R.China
2、Beijing General Station of Animal Husbandry, Beijing 100081, P.R.China

Received:2014-03-14 Online:2015-04-01 Published:2015-04-10
Contact: DIAO Qi-yu, Tel: +86-10-82106055, E-mail: diaoqiyu@caas.cn
About author:MA Tao, E-mail: matao0229@gmail.com;* These authors contributed equally to this study.
Supported by:
This study was supported by the Earmarked Fund for Beijing Dairy Industry Innovation Team, the Beijing Key Technology for Early Weaning of Calves, and the National Key Technology R&D Program of China (2012BAD12B06).

摘要/Abstract

摘要： This study aimed to investigate the effects of dietary supplementation of yeast β-glucan on the nutrient digestibility and serum profiles in pre-ruminant Holstein calves. Forty-two neonatal Holstein calves ((39.6±4.2) kg) were randomly allotted to six groups, and each was offered one of the following diets: a basal diet (control) or the basal diet supplemented with 25, 50, 75, 100 or 200 mg of yeast β-glucan kg–1 feed (dry matter basis). The basal diet consisted of a milk replacer and a starter feed. The trial lasted for 56 d. Two digestibility trials were conducted from d 14 to 20 and from d 42 to 48. Blood samples were collected on d 0, 14, 28 and 42 for serum profile analyses. On d 56, three calves from each group were slaughtered, and intestinal samples were collected to assess the villous height, crypt depth and mucosal thickness. Although feed intake was not affected by dietary treatment (P>0.05), the average daily gain (ADG) and gain-to-feed ratios were higher (P<0.05) for the calves fed 75 mg of yeast β-glucan kg–1 feed than those in the other groups. The supplementation of yeast β-glucan at 75 mg kg–1 feed increased the apparent digestibility of dry matter (DM), crude protein (CP), ether extract (EE), and phosphorus (P) (P<0.05) and the ratio of intestinal villous height to crypt depth (V/C) (P<0.05) when compared with the control group. No effects of yeast β-glucan on the serum concentrations of total protein (TP), albumin (ALB), serum urea nitrogen (SUN) and glucose (GLU) were observed (P>0.05). Compared with the control group, supplementation of yeast β-glucan decreased (P<0.05) the serum concentrations of triglycerides (TG) and total cholesterol (TC). The serum concentration of immunoglobulin G (IgG) and immunoglobulin M (IgM) increased quadratically (P<0.05), whereas the serum concentration of immunoglobulin A (IgA) was unaffected by dietary treatments (P>0.05). The supplementation of yeast β-glucan stimulated the enzymatic activity of alkaline phosphatase (ALP) (P<0.05) compared with the control group. The lysozyme (LYZ) concentration increased quadratically (P<0.05) with increasing yeast β-glucan levels. The results suggested that dietary supplementation of yeast β-glucan at 75 mg kg–1 feed improved nutrient digestibility, enhanced immunity by increasing the immunoglobulin concentration and stimulating ALP, and exerted no adverse effects on metabolism in pre-ruminant calves.

关键词: calf , digestibility , intestinal development , serum profile , yeast β-glucan

Abstract: This study aimed to investigate the effects of dietary supplementation of yeast β-glucan on the nutrient digestibility and serum profiles in pre-ruminant Holstein calves. Forty-two neonatal Holstein calves ((39.6±4.2) kg) were randomly allotted to six groups, and each was offered one of the following diets: a basal diet (control) or the basal diet supplemented with 25, 50, 75, 100 or 200 mg of yeast β-glucan kg–1 feed (dry matter basis). The basal diet consisted of a milk replacer and a starter feed. The trial lasted for 56 d. Two digestibility trials were conducted from d 14 to 20 and from d 42 to 48. Blood samples were collected on d 0, 14, 28 and 42 for serum profile analyses. On d 56, three calves from each group were slaughtered, and intestinal samples were collected to assess the villous height, crypt depth and mucosal thickness. Although feed intake was not affected by dietary treatment (P>0.05), the average daily gain (ADG) and gain-to-feed ratios were higher (P<0.05) for the calves fed 75 mg of yeast β-glucan kg–1 feed than those in the other groups. The supplementation of yeast β-glucan at 75 mg kg–1 feed increased the apparent digestibility of dry matter (DM), crude protein (CP), ether extract (EE), and phosphorus (P) (P<0.05) and the ratio of intestinal villous height to crypt depth (V/C) (P<0.05) when compared with the control group. No effects of yeast β-glucan on the serum concentrations of total protein (TP), albumin (ALB), serum urea nitrogen (SUN) and glucose (GLU) were observed (P>0.05). Compared with the control group, supplementation of yeast β-glucan decreased (P<0.05) the serum concentrations of triglycerides (TG) and total cholesterol (TC). The serum concentration of immunoglobulin G (IgG) and immunoglobulin M (IgM) increased quadratically (P<0.05), whereas the serum concentration of immunoglobulin A (IgA) was unaffected by dietary treatments (P>0.05). The supplementation of yeast β-glucan stimulated the enzymatic activity of alkaline phosphatase (ALP) (P<0.05) compared with the control group. The lysozyme (LYZ) concentration increased quadratically (P<0.05) with increasing yeast β-glucan levels. The results suggested that dietary supplementation of yeast β-glucan at 75 mg kg–1 feed improved nutrient digestibility, enhanced immunity by increasing the immunoglobulin concentration and stimulating ALP, and exerted no adverse effects on metabolism in pre-ruminant calves.

Key words: calf , digestibility , intestinal development , serum profile , yeast β-glucan

MA Tao, TU Yan, ZHANG Nai-feng, GUO Jiang-peng, DENG Kai-dong, ZHOU Yi, YUN Qiang, DIAO Qi-yu. Effects of dietary yeast β-glucan on nutrient digestibility and serum profiles in pre-ruminant Holstein calves[J]. Journal of Integrative Agriculture, 2015, 14(4): 749-757.

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Effects of dietary yeast β-glucan on nutrient digestibility and serum profiles in pre-ruminant Holstein calves

Effects of dietary yeast β-glucan on nutrient digestibility and serum profiles in pre-ruminant Holstein calves

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