Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population

doi:10.1016/S1671-2927(00)8541

Journal of Integrative Agriculture ›› 2012, Vol. 11 ›› Issue (2): 235-248.DOI: 10.1016/S1671-2927(00)8541

Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population

R V Chowda-Reddy, M Kirankumar, Susan E Seal, V Muniyappa, Girish B Val, M R Govindappa, John Colvin

1.Agriculture, Health and Environment Group, Natural Resources Institute, University of Greenwich at Medway, Chatham Maritime, Kent,
ME4 4TB, United Kingdom
2.Department of Plant Pathology, University of Agricultural Sciences, Bangalore-560 065, India
3.Department of Plant Pathology, Gujarat Agricultural University, Anand 388110, Gujarat, India
4.Southern Crop Protection and Food Research Centre, London-N5V 4T3, Canada

收稿日期:2010-03-15 出版日期:2012-02-01 发布日期:2012-02-11
通讯作者: R V Chowda-Reddy, Tel: +519-457-1470 (ext 284), Fax: +519-457-3997, E-mail: chowda.reddy@agr.gc.ca
作者简介:R V Chowda-Reddy, Tel: +519-457-1470 (ext 284), Fax: +519-457-3997, E-mail: chowda.reddy@agr.gc.ca
基金资助:
This publication is an output of Ph D work carried out by R. V. Chowda-Reddy (HEFCE fellowship) and Kiran Kumar, from the research projects funded by the University of Greenwich, together with the Department for International Development, UK (DFID project code R8247, Crop Protection Programme).

Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population

R V Chowda-Reddy, M Kirankumar, Susan E Seal, V Muniyappa, Girish B Val, M R Govindappa, John Colvin

1.Agriculture, Health and Environment Group, Natural Resources Institute, University of Greenwich at Medway, Chatham Maritime, Kent,
ME4 4TB, United Kingdom
2.Department of Plant Pathology, University of Agricultural Sciences, Bangalore-560 065, India
3.Department of Plant Pathology, Gujarat Agricultural University, Anand 388110, Gujarat, India
4.Southern Crop Protection and Food Research Centre, London-N5V 4T3, Canada

Received:2010-03-15 Online:2012-02-01 Published:2012-02-11
Contact: R V Chowda-Reddy, Tel: +519-457-1470 (ext 284), Fax: +519-457-3997, E-mail: chowda.reddy@agr.gc.ca
About author:R V Chowda-Reddy, Tel: +519-457-1470 (ext 284), Fax: +519-457-3997, E-mail: chowda.reddy@agr.gc.ca
Supported by:
This publication is an output of Ph D work carried out by R. V. Chowda-Reddy (HEFCE fellowship) and Kiran Kumar, from the research projects funded by the University of Greenwich, together with the Department for International Development, UK (DFID project code R8247, Crop Protection Programme).

摘要/Abstract

摘要： Bemisia tabaci adults from various host-plant species were collected from 31 regions across India. 266 B. tabaci samples were first screened by RAPD-PCR to examine molecular variability and to select individuals with different fingerprints. Host-plant and region of collection were then used to select 25 individuals for PCR amplification and sequencing of their partial mitochondrial cytochrome oxidase subunit one (mtCOI) genes. Pairwise comparisons with mtCOI consensus sequences showed that the majority of these samples had <3.5% sequence divergence from groups currently termed Asia I, Asia II-5, Asia II-7, and Asia II-8. The biotype-B B. tabaci from India clustered into the Middle East-Asia Minor 1 group. A new group of B. tabaci from Coimbatore, collected from pumpkin, was related most closely to the Asia I group (6.2% sequence divergence from the consensus Asia I sequence). To increase our understanding of the epidemiology of tomato leaf curl disease (ToLCD) and the key B. tabaci genetic groups involved in virus spread, the indigenous Asia I and the exotic biotype-B population from South India were used to carry out transmission experiments using Tomato leaf curl Bangalore virus (ToLCBV). The acquisition access periods (AAP), inoculation access periods (IAP), latent periods (LP), and ToLCBV transmission efficiencies of the two populations were compared and the biotype-B had the more efficient transmission characteristics. These results are discussed in relation to recent changes in the epidemiology of tomato leaf curl disease in South India.

关键词: begomoviruses, mitochondrial cytochrome oxidase, whitefly, Bemisia tabaci, virus-vector relationships, epidemiology

Abstract: Bemisia tabaci adults from various host-plant species were collected from 31 regions across India. 266 B. tabaci samples were first screened by RAPD-PCR to examine molecular variability and to select individuals with different fingerprints. Host-plant and region of collection were then used to select 25 individuals for PCR amplification and sequencing of their partial mitochondrial cytochrome oxidase subunit one (mtCOI) genes. Pairwise comparisons with mtCOI consensus sequences showed that the majority of these samples had <3.5% sequence divergence from groups currently termed Asia I, Asia II-5, Asia II-7, and Asia II-8. The biotype-B B. tabaci from India clustered into the Middle East-Asia Minor 1 group. A new group of B. tabaci from Coimbatore, collected from pumpkin, was related most closely to the Asia I group (6.2% sequence divergence from the consensus Asia I sequence). To increase our understanding of the epidemiology of tomato leaf curl disease (ToLCD) and the key B. tabaci genetic groups involved in virus spread, the indigenous Asia I and the exotic biotype-B population from South India were used to carry out transmission experiments using Tomato leaf curl Bangalore virus (ToLCBV). The acquisition access periods (AAP), inoculation access periods (IAP), latent periods (LP), and ToLCBV transmission efficiencies of the two populations were compared and the biotype-B had the more efficient transmission characteristics. These results are discussed in relation to recent changes in the epidemiology of tomato leaf curl disease in South India.

Key words: begomoviruses, mitochondrial cytochrome oxidase, whitefly, Bemisia tabaci, virus-vector relationships, epidemiology

R V Chowda-Reddy, M Kirankumar, Susan E Seal, V Muniyappa, Girish B Val, M R Govindappa, John Colvin. Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population[J]. Journal of Integrative Agriculture, 2012, 11(2): 235-248.

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Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population

Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population

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